Effects of bovine serum albumin (BSA) on the excited-state properties of meso-tetrakis(sulfonatophenyl) porphyrin (TPPS4)

Gonçalves, Pablo José; Bezerra, Fábio Castro; Almeida, Luciane Madureira; Alonso, Laís; Souza, Guilherme Rocha Lino de; Alonso, Antônio; Zílio, Sérgio Carlos; Borissevitch, Iouri

doi:10.1007/s00249-019-01397-w

Cited by 18 publications

(15 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both porphyrins and their Mn(III) complexes have been reported to bind with serum proteins, in particular albumin. [40][41][42] Additionally, several groups have reported high serum stability of Mn-porphyrins. Although, there is no reported data on the stability of Mn-(1), work carried out using the Mn-TPPS/Mn-(5) showed that the complex was stable in human plasma for up to 9 days based on UV-spectroscopy.…”

Section: Discussionmentioning

confidence: 99%

Microwave-assisted radiolabelling of 52Mn-porphyrins: applications in cell and liposome radiolabelling

Gawne

Pinto

Nielsen

et al. 2022

Preprint

View full text Add to dashboard Cite

Manganese porphyrins have several therapeutic/imaging applications, including their use as radioprotectants (in clinical trials) and as paramagnetic MRI contrast agents. The affinity of porphyrins for lipid bilayers also makes them candidates for cell/liposome labelling. We hypothesised that metalation with the positron emission tomography (PET) radionuclide 52Mn (t1/2 = 5.6 d) would allow long-term in vivo biodistribution studies of Mn-porphyrins as well as a method to label and track cells/liposomes, but methods for fast and efficient radiolabelling are lacking. Several porphyrins were produced and radiolabelled by addition to neutralised [52Mn]MnCl2 and heated using a microwave (MW) synthesiser and compared with non-MW heating. MW radiosynthesis allowed >95 % radiochemical yields (RCY) in just 1 h. Conversely, non-MW heating at 70 oC for 1 h resulted in low RCY (0 – 25 % RCY) and most porphyrins did not reach radiolabelling completion after 24 h. Formation of the 52Mn-complexes were confirmed with radio-HPLC by comparison with their non-radioactive 55Mn counterparts. Following this, several 52Mn-porphyrins were used to radiolabel liposomes resulting in 75 – 86 % labelling efficiency (LE). Two lead 52Mn-porphyrins were taken forward to label MDA-MB-231 cancer cells in vitro, achieving ca. 11 % LE. After 24 h, 32 – 45 % of the 52Mn-porphyrin was retained in cells. In contrast to standard methods, MW heating allows the fast synthesis of 52Mn-porphyrins with >95% radiochemical yields that avoid purification. 52Mn-porphyrins also show promising cell/liposome labelling properties. Our reported technique can potentially be exploited for the in vivo imaging of Mn-porphyrin therapeutics, as well as for the accurate in vivo quantification of Mn-porphyrin MRI agents.

show abstract

Section: Discussionmentioning

confidence: 99%

Microwave-assisted radiolabelling of 52Mn-porphyrins: applications in cell and liposome radiolabelling

Gawne

Pinto

Nielsen

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Both porphyrins and their Mn(III) complexes have been reported to bind with serum proteins, in particular albumin. [38][39][40] Despite this, several groups have reported high serum stability of Mnporphyrins. Although, there is no reported data on the stability of Mn-(1), work carried out using the isomer Mn-TPPS4 showed that the complex was stable in human plasma for up to 9 days based on UV-spectroscopy.…”

Section: Discussionmentioning

confidence: 99%

Microwave-assisted radiolabelling of 52Mn-porphyrins

Gawne

Pinto

Nielsen

et al. 2021

Preprint

View full text Add to dashboard Cite

Manganese porphyrins have several therapeutic/imaging applications; including their use as radioprotectants (in clinical trials), and as paramagnetic MRI contrast agents. The affinity of porphyrins for lipid bilayers also makes them candidates for cell/liposome labelling. We hypothesised that metalation with the positron emission tomography (PET) radionuclide 52Mn (t1/2 = 5.6 d) would allow long-term in vivo biodistribution studies of Mn-porphyrins as well as a method to label and track cells/liposomes, but methods for fast and efficient radiolabelling are lacking. Several porphyrins were produced and radiolabelled by addition to neutralised [52Mn]MnCl2 and heated at 165 oC for 1 h using a microwave (MW) synthesiser at a ligand concentration of 0.6 – 0.7 mM. These conditions were compared with non-MW heating at 70oC. MW radiosynthesis allowed >95 % radiochemical yields (RCY) in just 1 h. Conversely, non-MW heating at 70 oC for 1 h resulted in low RCY (0 – 25 % RCY) and most porphyrins did not reach completion after 24h. Formation of the 52Mn-complexes were confirmed with radio-HPLC by comparison with their non-radioactive 55Mn counterparts. Following this, several 52Mn-porphyrins were used to radiolabel liposomes by incubation at 50 oC for 30 min resulting in 75 – 86 % labelling efficiency (LE). Two lead 52Mn-porphyrins were taken forward to label MDA-MB-231 cancer cells in vitro, achieving ca. 11 % LE. After 24 h, 32 – 45 % of the 52Mn-porphyrin was retained in cells. In contrast to standard methods, MW heating allows fast synthesis of 52Mn-porphyrins with >95% radiochemical yields that avoid purification. 52Mn-porphyrins also show promising cell/liposome labelling properties. This technique can potentially be exploited for the in vivo imaging of Mn-porphyrin therapeutics, as well as for the accurate in vivo quantification of Mn-porphyrin MRI agents.

show abstract

“…As a special kind of proteins, serum albumins have strong ability to bind with porphyrins and other organic compounds [34][35][36][37]. Recently, some groups carried out researches on nonlinear optical and photophysical processes about meso-tetrakis(sulfonatophenyl) porphyrin (TPPS 4 ) bound to bovine serum albumin (BSA) [38,39]. These works aroused our interest on the nonlinear absorption behaviors of TPPS 4 binding with or without BSA.…”

Section: Introductionmentioning

confidence: 99%

Influence of bovine serum albumin (BSA) on photodynamic properties of meso-tetrakis(sulfonatophenyl) porphyrin (TPPS₄) for picosecond pulse trains

Miao¹,

Sun²,

Liu³

et al. 2023

Phys. Scr.

View full text Add to dashboard Cite

Due to the special interest and unique advantage of bovine serum albumin among various proteins, we carried out researches into effects of bovine serum albumin on photophysical characters of meso-tetrakis(sulfonatophenyl) porphyrin. The laser source we chose is picosecond pulse trains containing 20 subpulses of 70 picoseconds width separating by 13 nanoseconds at 532 nanometers. The theoretical analysis was carried out with a generalized five-level model representing the molecular populating structure. The two-dimensional paraxial field combined with the rate equations was solved by Crank-Nicholson numerical method. The nonlinear optical absorption mechanism in porphyrin is mainly two-step two-photon absorption. We found meso-tetrakis(sulfonatophenyl) porphyrin always shows prominent optical limiting effects binding with or without bovine serum albumin. In the presence of bovine serum albumin, the photophysical parameters of porphyrin get certain influence and cause slight weakening of optical limiting for porphyrin.

show abstract

Effects of bovine serum albumin (BSA) on the excited-state properties of meso-tetrakis(sulfonatophenyl) porphyrin (TPPS4)

Cited by 18 publications

References 41 publications

Microwave-assisted radiolabelling of 52Mn-porphyrins: applications in cell and liposome radiolabelling

Microwave-assisted radiolabelling of 52Mn-porphyrins: applications in cell and liposome radiolabelling

Microwave-assisted radiolabelling of 52Mn-porphyrins

Influence of bovine serum albumin (BSA) on photodynamic properties of meso-tetrakis(sulfonatophenyl) porphyrin (TPPS₄) for picosecond pulse trains

Contact Info

Product

Resources

About

Effects of bovine serum albumin (BSA) on the excited-state properties of meso-tetrakis(sulfonatophenyl) porphyrin (TPPS4)

Cited by 18 publications

References 41 publications

Microwave-assisted radiolabelling of 52Mn-porphyrins: applications in cell and liposome radiolabelling

Microwave-assisted radiolabelling of 52Mn-porphyrins: applications in cell and liposome radiolabelling

Microwave-assisted radiolabelling of 52Mn-porphyrins

Influence of bovine serum albumin (BSA) on photodynamic properties of meso-tetrakis(sulfonatophenyl) porphyrin (TPPS4) for picosecond pulse trains

Contact Info

Product

Resources

About

Influence of bovine serum albumin (BSA) on photodynamic properties of meso-tetrakis(sulfonatophenyl) porphyrin (TPPS₄) for picosecond pulse trains