Effects of bradykinin preconditioning in an experimental intestinal ischemia reperfusion model on rats

Dal, Fatih; Küçük, Can; Talih, Tutkun; Sözüer, Erdoğan; Topal, Uğur; Denız, Kemal

doi:10.1590/s0102-865020200040000002

Cited by 3 publications

(1 citation statement)

References 32 publications

(44 reference statements)

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“…BK-related peptides bind to B1 and B2 bradykinin receptors to activate a number of downstream targets such as nitric oxide (NO), cyclic guanosine monophosphate (cGMP), prostacyclin, and cyclic adenosine monophosphate (cAMP) [ 11 ]. Kininogens, kallikrein, kinins, kinin degrading enzymes, and kinin receptors constitute the kallikrein-kinin system (KKS) [ 12 ]. In various experimental animal disease models, stimulating the KKS through overexpression of KLK1 or other methods has been demonstrated to be beneficial to many diseases through its anti-inflammatory, antifibrotic, and antioxidative actions [ 11 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates

Zhang

Luo

Lin

et al. 2021

Oxidative Medicine and Cellular Longevity

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Objective. The aim of the present study was to investigate the protective effects and mechanisms of KLK1 on aging-related prostate alterations and search clues about the application of KLK1 to the treatment of human BPH. Methods. Thirty-six rats including 26 male wild-type SD rats and 10 transgenic rats were fed to 3- or 18-month-old and divided into three groups: young WTR (yWTR) as the control ( n = 16 ), aged WTR (aWTR) ( n = 10 ), and aged TGR (aTGR) ( n = 10 ). The prostates of the three groups of rats (10 rats per group) were harvested to evaluate the levels of KLK1 expression, oxidative stress, fibrosis, and involved signaling pathways, such as NO/cGMP, COX-2/PTGIS/cAMP, and TGF-β1/RhoA/ROCK1, via quantitative PCR, Western blot, histological examinations, and ELISA. Moreover, the remaining 6 yWTRs were sacrificed to obtain primary prostate fibroblast and aortic endothelial cells, and a coculture system was built with the cells for the verification of above signaling pathways in vitro. And the direct effects of bradykinin on prostate cells were detected by MTT experiment. Prostate specimens of 47 patients (age from 48 to 92 years) undergoing BPH surgery were collected after approval. Histological examinations and KLK1 IHC were preformed to analyze the relationship between KLK1 expression and age and prostate fibrosis. Results. The human KLK1 gene only existed and was expressed in aTGR. The prostate of young rats expressed more KLK1 than the aged and the expression of KLK1 in prostate decreased with age in humans ( r = − 0.347 , P = 0.018 ). Compared to the aWTR group, the yWTR and aTGR groups showed milder fibrosis, less oxidative stress, upregulated NO/cGMP, and COX-2/PTGIS/cAMP signaling pathways and inhibited TGF-β1/RhoA/ROCK1 signaling pathway. In the coculture system, KLK1 suppressed TGF-β1-mediated fibroblast-to-myofibroblast transdifferentiation via cleaving LMWK to produce the BK which upregulate eNOS expression and NO production in endothelial cells. BK not only slightly stimulated the proliferation ability of prostatic stromal cells but also upregulated iNOS and inhibited TGF-β1 expression in them. Conclusion. KLK1 protects prostate from oxidative stress and fibrosis via amplified NO/cGMP signal in aged rats. The decrease of KLK1 expression with aging is laying the groundwork for the application of KLK1 to the treatment of human BPH. The current experimental data showed that the side effects of KLK1 on the prostate cell were not obvious.

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Section: Introductionmentioning

confidence: 99%

Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates

Zhang

Luo

Lin

et al. 2021

Oxidative Medicine and Cellular Longevity

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Sleep Deprivation Impairs Intestinal Mucosal Barrier by Activating Endoplasmic Reticulum Stress in Goblet Cells

Li,

Gao,

Zhang

et al. 2024

The American Journal of Pathology

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Protective Effects of Hydrogen-Rich Saline on Experimental Intestinal Volvulus in Rats

Oral

Türkyılmaz

Karabulut

et al. 2022

Journal of Investigative Surgery

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Effects of bradykinin preconditioning in an experimental intestinal ischemia reperfusion model on rats

Abstract: Conceptıon and design of the study; acquisition, analysis and interpretation of data; technical procedures; statistics analysis; manuscript preparation and writing; final approval.

Cited by 3 publications

References 32 publications

Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates

Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates

Sleep Deprivation Impairs Intestinal Mucosal Barrier by Activating Endoplasmic Reticulum Stress in Goblet Cells

Protective Effects of Hydrogen-Rich Saline on Experimental Intestinal Volvulus in Rats

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