Effects of breed, postnatal development, and nutrition on mRNA expression of the FTO gene in porcine muscle and its relationship with intramuscular fat deposition

Abstract

ABSTRACT:The effects of breed, development, and nutrition on mRNA expression of the fat mass and obesityassociated gene (FTO) and its relationship with intramuscular fat (IMF) content in porcine muscle (m. longissimus dorsi; m.l.d.) were estimated. Purebred Jinhua, Zhongbai, Yorkshire, Duroc, Duroc × Zhongbai (DZ), and Duroc × Yorkshire × Landrace (DYL) pigs were used to investigate the effect of breed. Pigs weighing 2.5, 10, 20, 40, 60, and 100 kg were selected to study the effects of different stages of development. To study the effect of nutrition, four diets were selected: corn-soybean (CS), CS with 1.2% conjugated linoleic acid (CLA) or 0.05% creatine monohydrate (CMH), and barley-soybean (BS). All eighty animals were slaughtered, and m.l.d. samples were collected to examine FTO mRNA expression and IMF content. Results showed that breed significantly affected FTO mRNA expression and IMF content. FTO mRNA expression in the studied pigs was in the order: Zhongbai and Yorkshire > Duroc and DZ > Jinhua and DYL. The IMF content ordered by breed was Duroc > DZ > DYL > Jinhua > Zhongbai > Yorkshire. Both FTO mRNA expression and IMF content increased with age of the pigs, with the greatest difference seen between 100 kg pigs and all other weights. In the study, none of the four diets had a significant effect (P > 0.05) on FTO mRNA expression or IMF content. The study demonstrated that FTO mRNA expression increased with increasing body weight and was significantly affected by the breed of pigs. The results showed that FTO mRNA expression had an inconsistent correlation with IMF content between breeds and developmental ages.

Association of the ADRB3, FABP3, LIPE, and LPL gene polymorphisms with pig intramuscular fat content and fatty acid composition

“…Tao et al (2013) also presented the data that the IMF content was the highest in Duroc pigs. In the present study, the mean value of IMF contents (%) of Longissimus dorsi muscle in Shanzhu × Duroc crossbreds was 2.53.…”

Association between FTO gene polymorphism and productivity traits in Lithuanian pigs population

“…It might be that the FTO protein differs with different phenotypes and splice variants, which could be more related to intramuscular fat content in different breeds (Tao et al 2013).…”

Heterologous expression and purification of porcine fat mass andobesity-associated gene in Escherichia coli

“…A considerable number of studies concerning the porcine FTO gene have been reported (Du et al, 2009;Fan et al, 2009;Fontanesi et al, 2009Fontanesi et al, , 2010Zhang et al, 2009;Huang et al, 2010Huang et al, , 2011Tao et al, 2013). Numerous reports have provided evidence that the single nucleotide polymorphisms in the FTO gene are related to IMF deposition or IMF content in pigs (Fan et al, 2009;Fontanesi et al, 2009Fontanesi et al, , 2010Zhang et al, 2009).…”