Effects of bromopride on the healing of left colonic anastomoses in rats with induced abdominal sepsis

Silva, Silvana Marques e; Carneiro, Fabiana Pirani; Oliveira, Paulo Gonçalves de; Morais, Pedro Henrique Alves de; Silva, Naiara Galvão da; Sousa, João Batista de

doi:10.1590/s0102-86502012000600003

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…Conversely, in previous studies we found that administration of bromopride in the presence or absence of sepsis was associated with decreased tensile strength of left colonic anastomoses in rats 3 days after surgery, with no changes in adhesion formation ( 9 , 10 ), suggesting that other mechanisms may be involved. Therefore, the study of other healing parameters to elucidate the real action of bromopride on the healing process is needed.…”

Section: Introductioncontrasting

confidence: 63%

Effects of bromopride on expression of metalloproteinases and interleukins in left colonic anastomoses: an experimental study

Silva

Jerônimo

Silva-Pereira

et al. 2014

Braz J Med Biol Res

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Anastomotic dehiscence is the most severe complication of colorectal surgery. Metalloproteinases (MMPs) and interleukins (ILs) can be used to analyze the healing process of anastomosis. To evaluate the effects of bromopride on MMP and cytokine gene expression in left colonic anastomoses in rats with or without induced abdominal sepsis, 80 rats were divided into two groups for euthanasia on the third or seventh postoperative day (POD). They were then divided into subgroups of 20 rats for sepsis induction or not, and then into subgroups of 10 rats for administration of bromopride or saline. Left colonic anastomosis was performed and abdominal sepsis was induced by cecal ligation and puncture. A colonic segment containing the anastomosis was removed for analysis of gene expression of MMP-1α, MMP-8, MMP-13, IL-β, IL-6, IL-10, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ). On the third POD, bromopride was associated with increased MMP-1α, MMP-13, IL-6, IFN-γ, and IL-10 gene expression. On the seventh POD, all MMP transcripts became negatively modulated and all IL transcripts became positively modulated. In the presence of sepsis, bromopride administration increased MMP-8 and IFN-γ gene expression and decreased MMP-1, TNF-α, IL-6, and IL-10 gene expression on the third POD. On the seventh POD, we observed increased expression of MMP-13 and all cytokines, except for TNF-α. In conclusion, bromopride interferes with MMP and IL gene expression during anastomotic healing. Further studies are needed to correlate these changes with the healing process.

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Section: Introductioncontrasting

confidence: 63%

Effects of bromopride on expression of metalloproteinases and interleukins in left colonic anastomoses: an experimental study

Silva

Jerônimo

Silva-Pereira

et al. 2014

Braz J Med Biol Res

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“…The force at which the anastomosis is disrupted is then recorded. Either a simple commercial dynamometer[ 142 ] or more precise and expensive solutions can be used[ 77 ].…”

Section: Discussionmentioning

confidence: 99%

Experimental models of high-risk bowel anastomosis in rats: A systematic review

Ntampakis,

Pramateftakis,

Anestiadou

et al. 2024

World J Exp Med

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BACKGROUND Anastomotic leaks remain one of the most dreaded complications in gastrointestinal surgery causing significant morbidity, that negatively affect the patients’ quality of life. Experimental studies play an important role in understanding the pathophysiological background of anastomotic healing and there are still many fields that require further investigation. Knowledge drawn from these studies can lead to interventions or techniques that can reduce the risk of anastomotic leak in patients with high-risk features. Despite the advances in experimental protocols and techniques, designing a high-quality study is still challenging for the investigators as there is a plethora of different models used. AIM To review current state of the art for experimental protocols in high-risk anastomosis in rats. METHODS This systematic review was performed according to The Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. To identify eligible studies, a comprehensive literature search was performed in the electronic databases PubMed (Medline) and Scopus, covering the period from conception until 18 October 2023. RESULTS From our search strategy 102 studies were included and were categorized based on the mechanism used to create a high-risk anastomosis. Methods of assessing anastomotic healing were extracted and were individually appraised. CONCLUSION Anastomotic healing studies have evolved over the last decades, but the findings are yet to be translated into human studies. There is a need for high-quality, well-designed studies that will help to the better understanding of the pathophysiology of anastomotic healing and the effects of various interventions.

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“…-Effective treatment for preventing postoperative nausea and vomiting as well as chemotherapyinduced emesis [336]. -Was associated with decreased tensile strength and hydroxyproline concentration in colonic anastomoses in rats three days after surgery [337].…”

Section: Abbreviationsmentioning

confidence: 99%

Dopamine receptors in emesis: Molecular mechanisms and potential therapeutic function

Belkacemi

Darmani

2020

Pharmacological Research

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Effects of bromopride on the healing of left colonic anastomoses in rats with induced abdominal sepsis

Cited by 6 publications

References 16 publications

Effects of bromopride on expression of metalloproteinases and interleukins in left colonic anastomoses: an experimental study

Effects of bromopride on expression of metalloproteinases and interleukins in left colonic anastomoses: an experimental study

Experimental models of high-risk bowel anastomosis in rats: A systematic review

Dopamine receptors in emesis: Molecular mechanisms and potential therapeutic function

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