Effects of C-xylopyranoside derivative on epithelial regeneration in an <i>in vitro</i> 3D oral mucosa model

Uenoyama, Atsushi; Kakizaki, Ikuko; Shiomi, Aki; Saito, Natsukaze; Hara, Yuko; Saito, Taro; Ohnuki, Hisashi; Kato, Hiroko; Takagi, Ritsuo; Maeda, Takeyasu; Izumi, Kenji

doi:10.1080/09168451.2016.1153957

Cited by 6 publications

(2 citation statements)

References 61 publications

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“…Primary oral fibroblast cultures were established by an explant culture technique using the connected tissue after the epithelial layer was scraped off. Small explants were placed in a 60-mm Petri dish (Corning, New York, NY, USA) and incubated in Dulbecco's modified Eagle medium (DMEM; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), gentamicin, and amphotericin B as previously described [16].…”

Section: Primary Cell Culture Under Hypoxic Conditionsmentioning

confidence: 99%

Metabolomic Alteration of Oral Keratinocytes and Fibroblasts in Hypoxia

Kato

Sugimoto

Enomoto

et al. 2021

JCM

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The oxygen concentration in normal human tissue under physiologic conditions is lower than the atmospheric oxygen concentration. The more hypoxic condition has been observed in the cells with wound healing and cancer. Somatic stem cells reside in a hypoxic microenvironment in vivo and prefer hypoxic culture conditions in vitro. Oral mucosa contains tissue-specific stem cells, which is an excellent tissue source for regenerative medicine. For clinical usage, maintaining the stem cell in cultured cells is important. We previously reported that hypoxic culture conditions maintained primary oral keratinocytes in an undifferentiated and quiescent state and enhanced their clonogenicity. However, the metabolic mechanism of these cells is unclear. Stem cell biological and pathological findings have shown that metabolic reprogramming is important in hypoxic culture conditions, but there has been no report on oral mucosal keratinocytes and fibroblasts. Herein, we conducted metabolomic analyses of oral mucosal keratinocytes and fibroblasts under hypoxic conditions. Hypoxic oral keratinocytes and fibroblasts showed a drastic change of metabolite concentrations in urea cycle metabolites and polyamine pathways. The changes of metabolic profiles in glycolysis and the pentose phosphate pathway under hypoxic conditions in the oral keratinocytes were consistent with those of other somatic stem cells. The metabolic profiles in oral fibroblasts showed only little changes in any pathway under hypoxia except for a significant increase in the antioxidant 2-oxoglutaric acid. This report firstly provides the holistic changes of various metabolic pathways of hypoxic cultured oral keratinocytes and fibroblasts.

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Section: Primary Cell Culture Under Hypoxic Conditionsmentioning

confidence: 99%

Metabolomic Alteration of Oral Keratinocytes and Fibroblasts in Hypoxia

Kato

Sugimoto

Enomoto

et al. 2021

JCM

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“…Recientemente se encontró que el empleo de 2 o 10 mM de XPP (β-D-xylopyranosiden-propane-2-one) durante la elaboración de una MOIT podría aumentar el espesor promedio del epitelio sin afectar la estructura epitelial o su proceso de diferenciación, y estimular la expresión de decorina, sindecano-1, integrina α6, CD44, y los sustratos de señalización Akt/mTOR importantes en el crecimiento celular, síntesis de proteínas y espesor epitelial (29).…”

Section: Epitelios Delgados Y Sin Crestas Epitelialesunclassified

New challenges on the production of oral mucosa using Tissue Engineering Techniques

Bastidas¹,

Bello²

2016

Actual. Med.

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En los últimos años, la fabricación de tejidos artificiales mediante técnicas de Ingeniería Tisular ha sido de gran interés en el área de las ciencias de la salud. En concreto, en el campo odontológico y maxilofacial, la fabricación de una mucosa oral artificial completamente funcional ha sido un reto constante para la Ingeniería Tisular. En este sentido, aunque se han desarrollado diversos modelos de mucosa oral humana, aún existen carencias en lo que se refiere al mimetismo de los tejidos artificiales y los tejidos orales nativos. Dentro de dichas carencias destacan los tiempos prolongados del cultivo de queratinocitos, su baja capacidad de proliferación, la obtención de epitelios delgados y sin crestas epiteliales, la falta de vascularización en la zona implantada y la obtención de tejidos con una unión muco-cutánea para la reconstrucción del labio. El objetivo de esta revisión es mostrar los avances que hasta el momento se han obtenido en materia de la Ingeniería Tisular de la mucosa oral, así como discutir los retos por superar y sus posibles soluciones. AbstractIn recent years, the production of artificial tissues by Tissue Engineering techniques has been of great interest in the health sciences area. Specifically, in the dental and maxillofacial field. In this context, to develop a fully functional artificial oral mucosa is a constant challenge for Tissue Engineering. In this regard, several human oral mucosa models have been developed. However, prolonged periods of keratinocyte culture, low proliferation capacity, absence of rete ridges, and the lack of vascularization are the major shortcomings in this area. The objective of this review is to show the progress that so far have been obtained in the field of Tissue Engineering of the oral mucosa, as well as discuss the challenges ahead and possible solutions. Departamento de Histología, Universidad de GranadaEnviado : 17-09-2016 Revisado: 30-10-2016 Aceptado: 26-12-2016 Juliana Girón Bastidas Teléfono: 57 (1) Actual. Med. 2016; 101: (799): 176-182 INTRODUCCIÓNA pesar de que se han logrado grandes avances en la Ingeniería Tisular (IT) de la mucosa oral, hasta el momento no ha sido posible elaborar una mucosa oral generada por Ingeniería Tisular (MOIT) con todas las características funcionales propias de la mucosa oral humana, con un epitelio organizado, bien diferenciado y con una adecuada vascularización. En este contexto, se han detectado diferentes problemas en torno a la producción de una mucosa oral artificial, como por ejemplo los largos tiempos de cultivo para obtener queratinocitos, la baja capacidad de proliferación de las células epiteliales, la obtención de epitelios delgados y sin formación de crestas epiteliales, la falta de vascularización que conlleva al fracaso del implante y la obtención de tejidos con una unión mucocutánea para ser empleados en la reconstrucción del labio. El objetivo de esta revisión es mostrar los avances que hasta el momento se han obtenido en materia de la Ingeniería Tisular de la mucosa oral, así como discu...

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