Effects of cadmium and copper on antioxidant capacities, lignification and auxin degradation in leaves of pea (Pisum sativum L.) seedlings

Chaoui, Abdelilah; Ferjani, Ezzedine El

doi:10.1016/j.crvi.2004.10.001

Cited by 179 publications

(112 citation statements)

References 43 publications

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“…We also identified the same best three reference genes when considering Cu and Cd treatments separately, even though these metals have very different properties and effects towards the plants. Whereas Cu is redox-active and can directly induce oxidative stress, Cd is not redox-active and induces oxidative stress via indirect mechanisms (Chaoui and El Ferjani 2005;Romero-Puertas et al 2004). We therefore anticipate that the three best reference genes are also suitable for use when studying the effects of increased exposure of Arabidopsis thaliana to other metals.…”

Section: Discussionmentioning

confidence: 99%

Normalisation of real-time RT-PCR gene expression measurements in Arabidopsis thaliana exposed to increased metal concentrations

Remans

Smeets

Opdenakker

et al. 2008

Planta

307

225

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Accurate quantification by real-time RT-PCR relies on normalisation of the measured gene expression data. Normalisation with multiple reference genes is becoming the standard, but the best reference genes for gene expression studies within one organism may depend on the applied treatments or the organs and tissues studied. Ideally, reference genes should be evaluated in all experimental systems. A number of candidate reference genes for Arabidopsis have been proposed, which can be used as a starting point to evaluate their expression stability in individual experimental systems by available computer algorithms like geNorm and NormFinder. Using this approach, we identified the best three reference genes from a set of ten candidates -that included three traditional "housekeeping" genes -for normalisation of gene expression when roots and leaves of Arabidopsis thaliana are exposed to cadmium (Cd) and copper (Cu). The expression stabilities of AT5G15710 (F-box protein), AT2G28390 (SAND family protein), and AT5G08290 (mitosis protein YLS8) were the highest when considering the effect to the roots and shoots of Cd and Cu treatments. Even though the effect of Cd and excess Cu on the plants is very different, the same best reference genes were identified when considering Cd or Cu treatments separately. This suggests that these three genes may also be suitable when studying the gene expression after exposure of Arabidopsis thaliana to increased concentrations of other metals.

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Section: Discussionmentioning

confidence: 99%

Normalisation of real-time RT-PCR gene expression measurements in Arabidopsis thaliana exposed to increased metal concentrations

Remans

Smeets

Opdenakker

et al. 2008

Planta

307

225

View full text Add to dashboard Cite

show abstract

“…12 In contrast to Al, Cd-impeded growth is associated with the decreased auxin concentration by elevating IAA oxidase activity for an increase in auxin degradation. 30 This Cd-induced growth inhibition can be alleviated by salt application through decreasing Cd-induced elevation of IAA oxidase activity. 31 Auxin redistribution was also observed in Cd-treated roots, 18 however, the mechanisms underlying how excess Cd modulates the auxin redistribution remain elusive.…”

Section: Disclosure Of Potential Conflicts Of Interestmentioning

confidence: 99%

Role of ROS and auxin in plant response to metal-mediated stress

Yuan

Liu

Jin

et al. 2013

Plant Signaling & Behavior

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“…The inhibitory effects of Cd on various processes in adult P. sativum and germinating seeds have been previously documented (Chaoui et al 2004;Chaoui and El Ferjani 2005;Mihoub et al 2005;Smiri et al 2009Smiri et al , 2010aRahoui et al 2010). Bioassays reflect toxicological damage at biochemical and physiological levels.…”

Section: Discussionmentioning

confidence: 99%

Cadmium affects the NADP-thioredoxin reductase/thioredoxin system in germinating pea seeds

Smiri

Jelali

Ghoul

2013

Journal of Plant Interactions

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Seeds have devised sophisticated mechanisms to cope with adverse situations. Among these mechanisms the collectively referred to as redox homeostasis. We have investigated the thioredoxin (Trx)/NADPH/NADPHdependent thioredoxin reductase (NTR) systems during the pea (Pisum sativum L.) seed germination under heavy metal stress. Cotyledon and embryonic axis were analyzed between 0 and 5 days of germination in the presence or absence of cadmium (Cd). Under Cd stress conditions, NTR activity and Trx h3 and Trx h4 expression were stimulated, while the overall activity of Trx decreased. When incubated with Cd ions in vitro, the disulfide reductase activity of Trx h3 and f isoforms was drastically inhibited, probably through the formation of protein dimers as evidenced by the electrophoresis analysis. The NADPH status was also affected, since Cd treatment provoked an increase in oxidized state of coenzyme as compared to control redox ratio. The contribution of Trx system to the redox regulation during the germination is discussed in relation with the Cd-caused oxidative stress.

show abstract

Effects of cadmium and copper on antioxidant capacities, lignification and auxin degradation in leaves of pea (Pisum sativum L.) seedlings

Cited by 179 publications

References 43 publications

Normalisation of real-time RT-PCR gene expression measurements in Arabidopsis thaliana exposed to increased metal concentrations

Normalisation of real-time RT-PCR gene expression measurements in Arabidopsis thaliana exposed to increased metal concentrations

Role of ROS and auxin in plant response to metal-mediated stress

Cadmium affects the NADP-thioredoxin reductase/thioredoxin system in germinating pea seeds

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