Effects of Cadmium on Polyribosome Sedimentation Pattern in Mouse Liver

Car, Nikica; Naranscik, P.; Gamulin, Stjepan

doi:10.1159/000162943

Cited by 3 publications

(2 citation statements)

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“…The specific increase in the proportion of unprogrammed ribosomes in livers of cad mium-treated mice and the effect of cycloheximide which, by reducing the rate of elon gation, prevents cadmium-induced polyribo some disaggregation [Car et al" 1979], sug gest that cadmium provokes polyribosome disaggregation decreasing the rate of initia tion, relative to the rate of elongation of poly peptide chain synthesis. The effect of cad mium is not accompanied by changes in the proportion of native subunits, which suggests that the quantity of initiation factors neces sary for the existence of these ribosomal par ticles is not a rate-limiting factor in the pro posed inhibition of initiation.…”

Section: Discussionmentioning

confidence: 93%

“…The effect of cad mium is not accompanied by changes in the proportion of native subunits, which suggests that the quantity of initiation factors neces sary for the existence of these ribosomal par ticles is not a rate-limiting factor in the pro posed inhibition of initiation. It is less likely that hepatic polyribosome disaggregation in cadmium-treated mice is due to inhibition of mRNA synthesis, because it appears after 15 min [Car et al, 1979] which is consider ably shorter than the average half-life of eu karyotic mRNA species [Brawerman. 1974], Cadmium also provokes polyribosome disaggregation in isolated reticulocytes sug gesting a purely translational effect [Car et al" 1978], Lack of any change in the proportion of monoribosomes in the livers of cadmiumtreated mice indicates that the effect of cad mium is not accompanied by an increase in the RNA-ase activity.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Cadmium on Mouse Liver Polyribosome Function

Gamulin¹,

Narancsik²

1982

Pathobiology

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The distribution of ribosomes between their various functional states (native subunits, unprogrammed ribosomes, monoribosomes and polyribosomes) was analyzed in livers of cadmium-treated (CdCl₂ 20 µmol/kg, 1 h) and control mice. The ribosomes with double-labelled RNA were separated by sucrose density-gradient centrifugation in zonal rotor, and monomeric ribosome fraction was subsequently isolated and analyzed by selective dissociation of unprogrammed ribosomes. The analysis shows that the increase in monomeric ribosomes, occurring during polyribosome disaggregation in livers of cadmium-treated mice, is entirely due to the increase of the unprogrammed ribosome fraction at the expense of polyribosomes. Protein synthetic activity of polyribosomes in vivo in livers of cadmium-treated and control mice were compared by double labelling of nascent and soluble polypeptides with [¹⁴C]-and [³H]-leucine. Incorporation of radioactivity was relatively higher in nascent polypeptides and lower in soluble polypeptides in cadmium-treated as compared with control mice. The results indicate that cadmium inhibits protein synthesis in livers of mice, affecting both the rate of initiation and the rate of elongation, but decreasing the former more than the latter.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Effects of Cadmium on Mouse Liver Polyribosome Function

Gamulin¹,

Narancsik²

1982

Pathobiology

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The influence of tetracyclines on the aggregation of free and membrane-bound polysomes and on the RNA content of the liver of mice

Hopf

Böcker

1988

Naunyn-Schmiedeberg's Arch Pharmacol

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Effects of mercury bichloride on mouse kidney polyribosome structure and function

Pezerović¹,

Narancsik²,

Gamulin

1981

Arch Toxicol

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Polyribosome sedimentation pattern and their in vitro protein synthetic ability were investigated in kidneys of mice treated with a single injection of HgCl2. Mercury bichloride, after 1 h, evokes polyribosome disaggregation, the extent of which is logarithmically correlated with the dose in the range of 2.5-20 micromoles/kg. With the dose of 2.5 micromoles/kg the effect occurs in 1 h, it is maximal between 1 h and 3 h. After 6 h polyribosomes are reaggregated. Cycloheximide pretreatment does not prevent the HgCl2 induced disaggregation of kidney polyribosomes. The cell-free system derived from kidneys of HgCl2 treated mice (10 micromoles/kg, 1 h) has a decreased protein synthetic ability. Both, in livers of mice treated with 20 micromoles/kg HgCl2 and in isolated rat's reticulocytes incubated with 20 micro M HgCl2 during 1 h there were no apparent changes in the polyribosome sedimentation patterns.

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Effects of Cadmium on Polyribosome Sedimentation Pattern in Mouse Liver

Cited by 3 publications

References 10 publications

Effects of Cadmium on Mouse Liver Polyribosome Function

Effects of Cadmium on Mouse Liver Polyribosome Function

The influence of tetracyclines on the aggregation of free and membrane-bound polysomes and on the RNA content of the liver of mice

Effects of mercury bichloride on mouse kidney polyribosome structure and function

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