Effects of cafeteria diet feeding on β3-adrenoceptor expression and lipolytic activity in white adipose tissue of male and female rats

Lladó, Isabel; Estrany, ME; Rodrı́guez, Enrique; Amengual, Bartomeu; Roca, Pilar; Palou, Andreu

doi:10.1038/sj.ijo.0801390

Cited by 29 publications

(29 citation statements)

References 31 publications

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“…The results obtained in that study when increasing doses of NA were tested in the presence of RS-79948 (an α 2A -AR antagonist) point to differences in α 2A -AR function possibly contributing to the observed sex-dependent variation, together with a different β-AR distribution in gonadal adipose tissue of both sexes [28]. However, α 2A -AR-mRNA expression was not measured.…”

Section: Parametermentioning

confidence: 80%

“…Using the same model of overfeeding with cafeteria diet for 15 days, mature white adipocytes isolated from gonadal adipose tissue from males show higher lipolytic activity than those from females [28], probably due to differences in α 2A -AR. The results obtained in that study when increasing doses of NA were tested in the presence of RS-79948 (an α 2A -AR antagonist) point to differences in α 2A -AR function possibly contributing to the observed sex-dependent variation, together with a different β-AR distribution in gonadal adipose tissue of both sexes [28].…”

Section: Parametermentioning

confidence: 97%

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Sexual dimorphism in the adrenergic control of rat brown adipose tissue response to overfeeding

Rodrı́guez

Monjo

Rodríguez‐Cuenca

et al. 2001

Pflügers Arch - Eur J Physiol

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Gender-related differences in the brown adipose tissue (BAT) response to overfeeding rats on a cafeteria diet were studied by assessing the balance between the expression of beta-adrenoceptors (beta1-, beta2-, beta3-AR) and alpha2A-AR and their relation to the expression of uncoupling proteins (UCP1, UCP2, UCP3). Cafeteria diet feeding for 15 days, which involved a similar degree of hyperphagia in both sexes, led to a greater body weight excess in females than in males and a lower activation of thermogenesis. Gender-related differences were found for different adrenoceptor expression and protein levels, which might explain, in part, sex differences in the thermogenic parameters. The lower expression of alpha2A-AR in females than in males could be responsible for the higher expression of UCP1 and thermogenic capacity under non-hyperphagic conditions. However, in a situation of high adrenergic stimulation--as occurs with overfeeding--as there is a preferential recruitment of the beta3-AR by noradrenaline compared with other adrenergic receptors, the higher levels of beta3-AR in males rats than in females could be responsible for the greater thermogenic capacity and the lesser weight gain in males. Thus, the alpha2/beta3 balance in BAT could be a key in the thermogenic control.

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Section: Parametermentioning

confidence: 80%

Section: Parametermentioning

confidence: 97%

Sexual dimorphism in the adrenergic control of rat brown adipose tissue response to overfeeding

Rodrı́guez

Monjo

Rodríguez‐Cuenca

et al. 2001

Pflügers Arch - Eur J Physiol

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“…Sex-associated differences in rat brown adipose tissue (BAT) thermogenesis in response to diet-induced obesity and cold have been previously described, as have general sex differences in body weight gain and lipolytic activity of white adipose tissue (WAT) [1][2][3][4]. Sexual hormones have been proposed as key factors that could account in part for usually expressed only in BAT.…”

Section: Introductionmentioning

confidence: 99%

Opposite actions of testosterone and progesterone on UCP1 mRNA expression in cultured brown adipocytes

Rodríguez¹,

Monjo²,

Roca³

et al. 2002

CMLS, Cell. Mol. Life Sci.

105

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The brown adipose tissue (BAT) thermogenic response to diet-induced obesity and cold has been found to be gender dependent. In the present work, we aimed to investigate the effects of the main physiological male and female sex hormones, i.e. testosterone, progesterone and 17-beta-estradiol, on the expression of uncoupling protein I (UCP1)--the main mediator of BAT thermogenesis--and on UCP2 and lipid accumulation in rodent brown adipocytes differentiated in culture. Testosterone-treated cells showed fewer and smaller lipid droplets than control cells and a dose-dependent inhibition of UCP1 mRNA expression, under adrenergic stimulation by norepinephrine (NE). These effects were reverted by the androgen receptor antagonist flutamide, suggesting they are dependent, at least in part, on the androgen receptor. Progesterone- and 17-beta-estradiol-treated cells showed more and larger lipid droplets and progesterone stimulated NE-induced UCP1 mRNA expression at the lower concentration tested, but not at higher concentrations, suggesting that for brown adipocytes, this hormone is dose dependent. 17-beta-Estradiol did not have any remarkable effect either on UCP1 or UCP2 mRNA expression. Interestingly, the specific progesterone receptor antagonist RU486 induced UCP1 and UCP2 mRNAs, including UCP1 mRNA expression in non-NE-treated brown adipocytes, suggesting a profound effect of this antiprogestagen on brown adipocyte thermogenic capacity. Thus, are conclude that testosterone, 17-beta-estradiol, progesterone and RU486 have distinct actions on brown adipocytes, thus modulating UCP1 and UCP2 mRNA expression and/or lipid accumulation, and that sex hormones are factors that may explain in part the gender-dependent BAT thermogenic response.

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“…Thus, differences between men and women have been observed in the sensitivity to adrenergic stimulation [16,17], as well as in the rate of lipolysis induced by agents acting at different steps of the lipolytic pathway [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Depot- and Gender-related Differences in the Lipolytic Pathway of Adipose Tissue from Severely Obese Patients

Ramis

Salinas

García-Sanz

et al. 2006

Cell Physiol Biochem

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The present study was performed to analyze in detail gender- and site-related alterations in the adrenergic signal transduction pathway of lipolysis in fat cells isolated from subcutaneous abdominal and visceral fat depots from severely obese patients. The study group consisted of 30 morbidly obese subjects (9 men and 21 women) aged 41.1±1.9 years, with a body mass index (BMI) of 54.7±1.7 kg/m2, who had undergone abdominal surgery. Protein levels of hormone-sensitive lipase (HSL) and adrenergic receptors (AR), as well as HSL activity and the lipolytic response to adrenergic agents were analyzed. Both fat depots had similar basal lipolysis, but the capacity of catecholamines to activate lipolysis was greater in visceral fat, both at AR and postreceptor levels. Basal lipolysis and lipolytic activity induced by dibutyryl cyclic AMP were higher in men than in women. However, the visceral depot of women showed a higher maximal stimulation by noradrenaline than that of men, in accordance with higher ß1- and ß3-AR protein levels. In conclusion, the main gender-related differences were located in the visceral depot, with women exhibiting a higher sensitivity to catecholamines associated with an increased provision of ß-AR, while men showed an enhanced lipolytic capacity at the postreceptor level.

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Effects of cafeteria diet feeding on β3-adrenoceptor expression and lipolytic activity in white adipose tissue of male and female rats

Cited by 29 publications

References 31 publications

Sexual dimorphism in the adrenergic control of rat brown adipose tissue response to overfeeding

Sexual dimorphism in the adrenergic control of rat brown adipose tissue response to overfeeding

Opposite actions of testosterone and progesterone on UCP1 mRNA expression in cultured brown adipocytes

Depot- and Gender-related Differences in the Lipolytic Pathway of Adipose Tissue from Severely Obese Patients

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