Effects of caffeine intake during gestation and lactation on bones of young growing rats

Abstract

The objective of this study was to evaluate the extended effect of caffeine intake received during gestation and lactation on the mandible and femur of rats. Timed-pregnant dams were divided into two groups. Dams of group 1 were fed a 20% protein diet throughout the experimental period from day 9 of gestation. Dams of group 2 were also fed a 20% protein diet, supplemented with caffeine (lmg/100 g of body weight). Upon delivery, 8 pups were assigned to each dam, and the dams were continued on their respective diets. At weaning (day 22 postnatally), only male rats were selected. Pups of both groups were fed a 20% protein diet without caffeine. At day 56 postnatally the rats were killed. Mandibles and femurs were removed and the following parameters analyzed: weight, physical dimension, volume, and Knoop microhardness. Caffeine intake during gestation and lactation resulted in an impairment of femur growth and development and to a lesser extent mandibular growth and development. The early effects of caffeine in the maternal diet were lasting, as noted by the lack of recovery of the offspring even after changing to a caffeine-free diet for an extended time after weaning.

Favorable effect of moderate dose caffeine on the skeletal system in ovariectomized rats

“…Also experimental studies did not provide conclusive results on the effects of coffee/caffeine on the skeletal system. Although deleterious effects of caffeine have been reported in rats [14][15][16][17][18][19][20][21][22][23][24], it has been observed that long-term administration of coffee in the diet did not damage the rat skeletal system [25].…”

Elucidating the role of size of hydroxyl apatite particles toward the development of competent antiosteoporotic bioceramic materials: In vitro and in vivo studies

“…29 Density can be calculated by using the relation given below; Entire femur has been dehydrated with ethanol.…”

“…Density of the solid (bone) can be determined by volume displacement because submerged object displaces a volume equal to its own volume. 29 Density can be calculated by using the relation given below;…”

53: Is Caffeine a Risk Factor for Osteopenia of Prematurity?

“…In a study examining the effect of maternal caffeine intake on fetal bone, Nakamato et al (1999) found that, normal growth and development of the fetal bone are impaired as a result of maternal caffeine intake (Nakamoto, Grant & Yazdani, 1999). The early effects of caffeine in the maternal diet were lasting as noted by the lack of recovery of the offspring even after changing to a caffeine-free diet (Schneider, Miller & Nakamoto, 1990). These results may grant some backing to our study giving that caffeine in the form of coffee, tea and cola are among the most consumable drinks in the world, and most of the study population was fed either expressed breast milk or donor breast milk.…”