Effects of calcium silicate‐based cements on odonto/osteogenic differentiation potential in mesenchymal stem cells

Şişmanoğlu, Soner; Ercal, Pınar

doi:10.1111/aej.12615

Cited by 7 publications

(5 citation statements)

References 28 publications

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“…In the defect areas where nMTA was placed, immature bone formation was observed at a rate of 54.3% on the 7th day and 28.6% on the 14th day. The remarkable results of our research on nMTA corroborate those of many studies that have highlighted the superior properties of the material 9 , 37 , 38 .…”

Section: Discussionsupporting

confidence: 89%

The osteoinductive potential of different root-end filling materials in a rat femur model

Aksu,

Delikan,

Küçük

et al. 2024

Sci Rep

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In pediatric dentistry, the enduring success of root-end filling materials employed for the establishment of apical plugs in immature teeth undergoing endodontic intervention is contingent upon their possessing a robust osteoinductive capacity. Hence, the primary objective of this study was to histologically assess the osteoinductive potential of four distinct dental materials, specifically recommended for retrograde filling applications, utilizing an animal hard tissue model. Within the designed experimental model, two bone defects measuring 2 mm in diameter and 2 mm in depth were created in both femurs of a cohort comprising 21 male Wistar albino rats. The first defect in the right femur was left blank as the control group, and Neo MTA Plus was placed in the second defect. The EndoSequence BCRRM Fast Set Putty was placed in the first of the defects on the left femur, and Endo Repair was placed in the second defect. Subjects were sacrificed after 7, 14, and 28 days of follow-up, and sections were examined to assess the degree of inflammation, connective tissue formation, and new bone formation. The data were statistically evaluated with Kruskal‒Wallis and post hoc Dunn's tests using SPSS 12 software. The bone healing levels of the Neo MTA Plus group were significantly higher than those of the other groups in all periods (p < 0.05). Bone organization in all groups decreased over time, and fibrous tissue was enriched. The results of this study demonstrate that NeoMta Plus has superior osteoinductive properties compared to other materials but that EndoSequence and Endo Repair have the potential to be developed.

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Section: Discussionsupporting

confidence: 89%

The osteoinductive potential of different root-end filling materials in a rat femur model

Aksu,

Delikan,

Küçük

et al. 2024

Sci Rep

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“…Isolated from the tooth germs of third molars, which can be considered a more primitive tissue present during young adulthood compared to more developed pulp and apical papilla tissues, tooth germ-derived MSCs are multipotent stem cells that can benefit from interactions between ectoderm and mesoderm as they are derived from epithelial cells and the underlying mesenchyme [15]. Recent studies reported a high osteo/odontogenic potential for tooth germ-derived MSCs both in vivo and in vitro, alone or in combination with scaffolds [11,13,16]. Hence, the present study used tooth germ-derived MSCs to demonstrate if MTA-based biomaterials can support the induction of odontogenesis and maintain viability.…”

Section: Discussionmentioning

confidence: 99%

“…Samples were prepared as discs 5 mm in diameter and 3 mm thick in pre-sterilized Teflon ® molds in a laminar flow under aseptic conditions using the manufacturer's recommendations. Material extracts were prepared as previously described [13]. All prepared samples were incubated at 37 • C for 6 h. Samples were exposed to UV light at room temperature for 1 h. After sterilization, sample discs were submerged into 1 mL of growth medium and kept in a 90% humidified atmosphere of 5% CO 2 for a day.…”

Section: Preparation Of Materials Extractsmentioning

confidence: 99%

Biocompatibility and Osteo/Odontogenic Potential of Various Bioactive Root-End Filling Materials

Ercal,

Sismanoglu

2023

Applied Sciences

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This in vitro study aimed to investigate if different bioactive root-end filling materials can promote osteo/odontogenic differentiation of mesenchymal stem cells (MSCs) and support their viability. MSCs from porcine tooth germs were isolated. Cells were exposed to extracts from MTA Angelus, BIOfactor MTA, Medcem MTA, Well-Root ST, and Pure Portland Cement for 7 days. Viability was determined with MTS and live/dead assay. Osteo/odontogenic differentiation was evaluated with alkaline phosphatase (ALP) activity and quantitative real-time PCR (RUNX2, DMP-1, and DSPP genes) which were compared with osteo/odontogenic-induced MSCs and non-treated cells. All the tested materials supported cell proliferation and cells maintained their viability after 7 days. Osteo/odontogenic differentiation of MSCs was promoted by the tested materials in varying levels as demonstrated by increased ALP activity and upregulation of related gene markers in comparison to the control group. Pure Portland Cement demonstrated a continuous high ALP activity on day 7, showing the highest value among all materials and significantly increased in comparison to the control group (p < 0.001). Significant RUNX2 expression and high ALP activity (p < 0.001) similar to that of osteogenically induced cells was detected for Pure Portland Cement after 7 days. Tested MTA-based cement materials are biocompatible and induce osteo/odontogenic differentiation in vitro. MTA materials performed similarly to Pure Portland Cement regarding osteo/odontogenic differentiation.

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“…The findings related to Biodentine could be explained by its ability to induce differentiation of pulp stem cells and increased expression of dentin sialoprotein and alkaline phosphatase activity 30) . Complete or partial dentin bridges were primarily detected during 4 weeks, with enhanced continuity and thickness by 8 weeks.…”

Section: Discussionmentioning

confidence: 99%

Regenerative potential of a novel aloe vera modified tricalcium silicate cement as a pulp capping material: An animal study

SEIF,

ELBANNA,

ABU-SEIDA

et al. 2023

Dent. Mater. J.

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This study compared the histologic response of a pulp capping material Matreva MTA modified with different concentrations of aloe vera (AV) solutions to Biodentine cement. Ninety dogs' teeth were included and categorized according to the capping material into five groups (18 teeth each); Group I (Biodentine), group II (Matreva MTA), group III (Matreva MTA 10% AV), group IV (Matreva MTA 20% AV) and group V (Matreva MTA 30% AV). The histopathological findings were recorded at 2, 4, and 8 weeks. Matreva MTA and Biodentine groups showed the highest inflammatory cell count compared to the AV-modified Matreva MTA groups at 2-and 4-week intervals (p>0.05). Moreover, the AV-modified Matreva MTA and Biodentine groups showed higher dentin bridge thickness compared to unmodified Matreva MTA at different follow-up periods (p<0.05). AV can significantly enhance the in vivo bioactivity of Matreva MTA, inducing mild inflammation and good dentine bridge formation comparable to Biodentine.

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Effects of calcium silicate‐based cements on odonto/osteogenic differentiation potential in mesenchymal stem cells

Cited by 7 publications

References 28 publications

The osteoinductive potential of different root-end filling materials in a rat femur model

The osteoinductive potential of different root-end filling materials in a rat femur model

Biocompatibility and Osteo/Odontogenic Potential of Various Bioactive Root-End Filling Materials

Regenerative potential of a novel aloe vera modified tricalcium silicate cement as a pulp capping material: An animal study

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