Effects of Carbon Dioxide and Low Temperature Narcosis on Honey Bees,
Apis mellifera1

Ebadi, R.; Gary, Norman E.; Lorenzen, Kenneth

doi:10.1093/ee/9.1.144

Cited by 60 publications

(48 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Anaesthesia can affect bees' behaviour, for example B. terrestris workers were found to evict larvae following CO 2 narcotisation (Pomeroy and Plowright, 1979). In honeybees, CO 2 exposure of 30 seconds or more has been found to reduce pollen gathering (Ebadi et al, 1980). We did not compare the behaviour of previously narcotised bumblebee foragers with untreated ones, but the proportion of foragers bringing in pollen loads was no lower at the end of the trapping period than at the beginning.…”

Section: Forager Trapsmentioning

confidence: 91%

A modular system for trapping and mass-marking bumblebees: applications for studying food choice and foraging range

et al. 2006

View full text Add to dashboard Cite

-Two new techniques for the study of bumblebee behavioural ecology are described. Interchangeable nest entrance modules allow (i) unimpeded bee traffic, (ii) trapping of incoming foragers for counting and removal of pollen loads; or (iii) colour marking of bees leaving the colony using dye powder. The forager traps captured all returning foragers while in place and 35% of them were carrying pollen loads. During the four week experiment, the percentage of mixed pollen loads decreased and the proportion of pollen loads from mass-flowering resources increased. The dye dispensers automatically marked 86% of foragers as they left the colony (approximately 28 bees per hour), and 37% of returning bees were marked. Different colours were used for bees in each colony, which could then be observed in the field.Bombus / pollen collection / mark-reobservation / foraging patterns / mass marking / experimental device

show abstract

Section: Forager Trapsmentioning

confidence: 91%

A modular system for trapping and mass-marking bumblebees: applications for studying food choice and foraging range

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Foragers typically return to the same source of pollen or nectar over the course of days (Seeley, 1995) and in being strongly susceptible to substances that potentially could harm the colony unit, an individual forager dies before she can bring substantial amounts of contaminants to the hive. A wide range of physiological stressors, such as cooling, oxygen deprivation, mechanical wounding, and parasite exposure, cause nurse bees to differentiate into foragers (Bühler et al, 1983;Ebadi et al, 1980;Kovac and Crailsheim, 1988;Tustain and Faulke, 1979). Thereby, individuals in poor condition can also be driven into a physiological state in which they rapidly perish (Amdam et al, 2004b).…”

Section: A Programmed Disposal Of Somamentioning

confidence: 99%

Order, Disorder, Death: Lessons from a Superorganism

Amdam¹,

Seehuus²

2006

Advances in Cancer Research

View full text Add to dashboard Cite

Animal models contribute to the understanding of molecular mechanism of cancer, revealing complex roles of altered cellular-signaling networks and deficient surveillance systems. Analogous pathologies are documented in an unconventional model organism that receives attention in research on systems theory, evolution, and aging. The honeybee (Apis mellifera) colony is an advanced integrative unit, a "superorganism" in which order is controlled via complex signaling cascades and surveillance schemes. A facultatively sterile caste, the workers, regulates patterns of growth, differentiation, homeostasis, and death. Workers differentiate into temporal phenotypes in response to dynamic social cues; chemosensory signals that can translate into dramatic physiological responses, including programmed cell death. Temporal worker forms function together, and effectively identify and terminate abnormal colony members ranging from embryos to adults. As long as this regulatory system is operational at a colony level, the unit survives and propagates. However, if the worker phenotypes that collectively govern order become too few or change into malignant forms that bypass control mechanisms to replicate aberrantly; order is replaced by disorder that ultimately leads to the destruction of the society. In this chapter we describe fundamental properties of honeybee social organization, and explore conditions that lead to states of disorder. Our hope is that this chapter will be an inspirational source for ongoing and future work in the field of cancer research.

show abstract

“…It is known, that the hemolymph juvenile hormone (JH) titers rise in the transition from hive to fi eld workers (Jaycox, 1976;Huang and Robinson, 1996;Amdam and Omholt, 2003). According to Ebadi et al (1980) the treatment with CO 2 causes the same behavioral changes as the JH. Therefore, it seems that the gas effect in the workers mimics the rising of the JH titers (Engels et al, 1976;Koywiwattrakul et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…The analysis of the ovaries' morphology is shown in Table 1. The exposure time was based on the studies of Ebadi et al (1980) and Berger and Abdalla (2004) where different times of CO 2 exposure and longevity were tested. The workers analysed at 5 days-old were exposed twice: when newly emerged and at 3 days-old (and analysed at 5 days-old, two days after the second exposure).…”

Section: Co 2 Expositionmentioning

confidence: 99%

“…The exposition of A. mellifera newly emerged workers to a saturated CO 2 atmosphere promotes several changes: physiological, as inhibition of the ovaries, hypopharyngeal, and wax glands development (Fyg, 1950;Skowronek and Jaycox, 1974;Harris and Harbo, 1990) and behavioral, as a precocious acquirement of the forager activities. Moreover, there is a decrease in the lifespan, pollen collection, and consumption (Skowroneck and Jaycox, 1974;Ebadi et al, 1980;Berger and Abdalla, 2004). The effect on the ovaries was attributed to a decreased synthesis of vitellogenin (Vg), which is dependent on the intake of pollen (Bitondi and Simões, 1996).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ovary Development in Honeybee (Apis Mellifera L.) Workers Under Co₂ Narcosis, Caged Outside of the Colony

Berger

Poiani

Roat

et al. 2015

Journal of Apicultural Science

View full text Add to dashboard Cite

A b s t r a c t Exposure of A. mellifera workers to CO 2 has been reported to decrease life span, induce behavioral changes, and inhibit the development of some glands and the ovaries. However, the use of CO 2 is currently practiced among beekeepers and how the development of workers' ovaries are affected is unknown. The present work aimed to evaluate the effect of CO 2 on the ovaries of A. mellifera workers, using a morphological approach. Newly emerged, and 3, 5, and 10-day-old workers were exposed to saturated CO 2 for 30 seconds, more than once. The ovaries were examined under light (LM) and transmission electron microscopy (TEM). The ovaries of narcotized, 5-day-old workers exhibited long ovarioles with well-developed germarium, although oogenesis showed little development. While in the control group, the ovaries already exhibited cysts with cystocytes forming rosettes. At 10-days-old, the ovaries feature was variable; some of the ovaries showed ovarioles with many rosette cysts while others showed a high intensity of disorganization due to the beginning of cell death. The ovarioles of the ovaries of 15-day-old treated workers were morphologically varied as oogenesis showed little or no advance. In others cases, a complete disorganization with swollen cells and cell death features were observed. Germ cells of the ovaries of CO 2 -treated workers exhibited well-structured organelles, such as the Golgi complex, and larger amounts of mitochondria in the cytoplasm than the control group, but there was disorganization of the endoplasmic reticulum membranes. Our fi ndings revealed that the exposure of workers to CO 2 promotes impairment of oogenesis and ovarian degradation.

show abstract

Effects of Carbon Dioxide and Low Temperature Narcosis on Honey Bees, Apis mellifera1

Cited by 60 publications

References 0 publications

A modular system for trapping and mass-marking bumblebees: applications for studying food choice and foraging range

A modular system for trapping and mass-marking bumblebees: applications for studying food choice and foraging range

Order, Disorder, Death: Lessons from a Superorganism

Ovary Development in Honeybee (Apis Mellifera L.) Workers Under Co₂ Narcosis, Caged Outside of the Colony

Contact Info

Product

Resources

About

Effects of Carbon Dioxide and Low Temperature Narcosis on Honey Bees, Apis mellifera1

Cited by 60 publications

References 0 publications

A modular system for trapping and mass-marking bumblebees: applications for studying food choice and foraging range

A modular system for trapping and mass-marking bumblebees: applications for studying food choice and foraging range

Order, Disorder, Death: Lessons from a Superorganism

Ovary Development in Honeybee (Apis Mellifera L.) Workers Under Co2 Narcosis, Caged Outside of the Colony

Contact Info

Product

Resources

About

Ovary Development in Honeybee (Apis Mellifera L.) Workers Under Co₂ Narcosis, Caged Outside of the Colony