Effects of Carbon Dioxide on the Colonial Growth of Pathogenic Leptospirae

Yanagawa, Ryo; Hiramune, Takashi; Fujita, J.

doi:10.1128/jb.85.4.875-880.1963

Cited by 12 publications

(3 citation statements)

References 8 publications

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“…As Yanagawa, Hiramune, and Fujita (1963) have reported recently, colonial leptospiral growth was not obtained in the absence of C02 or in the presence of 10% or more CO2 in the air. When the colonial variants were grown in a semisolid medium containing 0.4% agar, no differences were found in the number or distribution of bands of growth (Dinger's bands).…”

Section: Resultssupporting

confidence: 54%

Leptospiral Colonial Morphology

Stalheim

Wilson

1963

J Bacteriol

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Stalheim , O. H. V. (University of Wisconsin, Madison) and J. B. Wilson . Leptospiral colonial morphology. J. Bacteriol. 86: 482–489. 1963.—A sequence of apparent colonial types was observed with colonies of Leptospira pomona, L. canicola, L. icterohaemorrhagiae , and L. grippotyphosa in agar medium. Although some colonies of these serotypes had a different appearance initially, they eventually developed the mature or final appearance characteristic of the serotype. Colonies of freshly isolated, virulent cultures of L. pomona, L. canicola , and L. icterohaemorrhagiae were similar in appearance to colonies of avirulent strains of the same serotype. Additional studies of three stable and distinct colonial types of a laboratory strain of L. autumnalis revealed no differences in antigenicity, catalase activity, or mouse infectivity; however, differences in susceptibility to lysis by oleic acid were found. Although the colonial variants were stable during several in vitro variations, including growth in the presence of homologous antiserum and mutation to growth in a chemically characterized medium, rapid dissociation in vivo was found.

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Section: Resultssupporting

confidence: 54%

Leptospiral Colonial Morphology

Stalheim

Wilson

1963

J Bacteriol

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“…However, supplementation with serum, as routinely used for EMJH, might be beneficial. The added benefit of incubating cultures in the presence of 1-5% CO 2 also needs to be addressed 27,28 . Finally, a rigorous evaluation to more completely chemically define multiple sources of BSA, and its fatty acids fractions, is required to identify correlations between the presence/absence of fatty acids in BSA preparations that support/inhibit growth of specific serovars/species compared to others.…”

Section: Discussionmentioning

confidence: 99%

Isolation and propagation of leptospires at 37 °C directly from the mammalian host

Hornsby

Alt

Nally

2020

Sci Rep

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The causative agent of leptospirosis includes multiple serovars and species of pathogenic leptospires that are excreted via urine from reservoir hosts of infection. Primary isolation takes weeks to months, and is limited to semi-solid media at 28-30 °C. Here we present an alternative media formulation, HAN, compared to commercially available EMJH and the more specialized T80/40/LH media formulations, in semi-solid and liquid compositions, for the primary isolation of two diverse species and serovars of pathogenic leptospires directly from host kidney tissue. All three media types supported the isolation and propagation of L. interrogans serovar Copenhageni strain IC:20:001 in semi-solid media at 29 °C. However, only HAN and T80/40/LH supported the growth of L. borgpetersenii serovar Hardjo strain HB15B203 at 29 °C. In addition, HAN supported primary isolation at 37 °C. Both T80/40/LH and HAN supported primary isolation of strain IC:20:001 in liquid media at 29 °C but only HAN supported growth of strain HB15B203 in liquid media, at both 29 and 37 °C. HAN media supports the primary isolation of fastidious pathogenic leptospires directly from infected host tissue at either 29 or 37 °C: this formulation represents a more defined media for the continued optimization of growth factors required to support the primary isolation of the large and diverse range of species and serovars within the genus Leptospira circulating within domestic and wild animal populations.

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“…Chemotaxis of leptospiras to such substances should be studied. There have been a few reports on the enhancement of growth of leptospiras in medium in the presence of substances such as hemoglobin (2, 6), iron (3) and carbon dioxide (9). These studies, however, did not clarify whether there was leptospiral chemotaxis to these substances.…”

Section: Introductionmentioning

confidence: 99%

A Basic Study of Chemotaxis of Leptospiras

Hiramune

Shiraiwa

Kikuchi

et al. 1990

Journal of Veterinary Medicine, Series B

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Summary Chemotaxis of leptospiras was studied using a U‐tube made of a commercially available straw‐shaped polypropylene tube with an uneven flexible part. The U‐tube was made by folding the polypropylene tube double and binding the vertical parts by a piece of tape, thus making the uneven flexible part the bottom. Semi‐solid Korthof's medium was poured into the U‐tube. Preliminary experiments showed that the medium containing 0.5 % agar and measuring 2 cm in length was appropriate. Leptospiras inoculated on the top of the medium of one side of the U‐tube moved toward the top of the medium of the other side. The leptospiral move toward the uninoculated top was significantly faster when 6 μg of hemoglobin were added to the uninoculated top. Chemotaxis of leptospiras to hemoglobin was thus shown, which is the first evidence of chemotaxis of leptospiras.

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Effects of Carbon Dioxide on the Colonial Growth of Pathogenic Leptospirae

Cited by 12 publications

References 8 publications

Leptospiral Colonial Morphology

Leptospiral Colonial Morphology

Isolation and propagation of leptospires at 37 °C directly from the mammalian host

A Basic Study of Chemotaxis of Leptospiras

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