Effects of Carbon Dioxide on Growth and Maltose Fermentation by
            <i>Bacteroides amylophilus</i>

Caldwell, Daniel R.; Keeney, Mark; Soest, P.J. Van

doi:10.1128/jb.98.2.668-676.1969

Cited by 29 publications

(6 citation statements)

References 33 publications

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“…Carbon dioxide can be fixed by many hetrotrophs. It has been shown by Gaffney (11) ) reduce the lag period in aerobic catabolism of lucose and by Caldwell et al (6) to be incororated into the carboxyl function of the sucinate produced by Bacteroides ruminicola uring glucose fermentation. To determine rhether the asparaginase synthesis associated rith reduced oxygen tension might actually be a onsequence of carbon dioxide accumulation, te sparged carbon dioxide through the culture iedium during the aging period, but observed no ffect.…”

Section: Resultsmentioning

confidence: 97%

Effect of Glucose and Low Oxygen Tension on l-Asparaginase Production by a Strain of Escherichia coli B

Boeck¹,

Squires²,

Wilson³

et al. 1970

Applied Microbiology

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Section: Resultsmentioning

confidence: 97%

Effect of Glucose and Low Oxygen Tension on l-Asparaginase Production by a Strain of Escherichia coli B

Boeck¹,

Squires²,

Wilson³

et al. 1970

Applied Microbiology

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“…Carbon dioxide metabolism during fermentation. Caldwell et al (11) showed that 10% carbon dioxide in the gaseous phase did not support growth, but 20% carbon dioxide supported full growth of B. amylophilus. B. ruminicola shows a similar requirement for carbon dioxide (Fig.…”

Section: Resultsmentioning

confidence: 99%

Metabolism and growth yields in Bacteroides ruminicola strain b14

Howlett¹,

Mountfort²,

Turner³

et al. 1976

Appl Environ Microbiol

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Metabolism of D-glucose by Bacteroides ruminicola subsp. brevis, strain B,4, has been examined. Growth yield studies gave molar growth yields, corrected for storage polysaccharide, of approximately 66 g (dry weight)/mol of glucose fermented. The storage polysaccharide amounted to about 14% of the total dry weight, or 55% of the total cellular carbohydrate, at full growth. After correcting glucose utilization for incorporation into cellular carbohydrate, measurement of product formation showed that 1.1 succinate, 0.8 acetate, and 0.35 formate are produced and 0.5 CO2 net is taken up during the fermentation of 1 glucose under the conditions used. The implication of these results with respect to adenosine 5'-triphosphate (ATP) molar growth yield calculations is discussed. If substratelevel phosphorylation reactions alone are responsible for ATP generation, then the ATP molar growth yield must be about 23 g (dry weight)/mol of ATP. Alternatively, if anaerobic electron transfer-linked phosphorylation also occurs, the ATP molar growth yield will be lower. Bacteroides ruminicola is one of the more numerically important bacteria found in the rumen, representing 6 to 19% of the total culturable carbohydrate fermenters present (8). It is not immediately obvious why B. ruminicola can compete so successfully in this anaerobic environment, which contains so many other carbohydrate-utilizing, organic acid-producing bacteria. Part of the reason for its quantitative predominance may be the result of its great versatility towards possible carbohydrate and nitrogen sources utilized (7, 8). However, it is also likely that the species has a highly efficient energy metabolism and can multiply rapidly. This study set out to examine growth yields of B. ruminicola, as an index of its energy metabolism, to discover whether the yields are "normal" or "anomalous" (19) and can be predicted from the products of fermentation. Careful attention has been paid to correction for storage polysaccharide and carbon recovery in products, which can both lead to erroneous results if ignored. B. ruminicola has a b-type cytochrome, which may be involved in fumarate reduction by reduced nicotinamide adenine dinucleotide (40); such electron transport systems in other bacteria have sometimes been postulated to be coupled to anaerobic electron transport phosphorylation reactions (3, 16, 28). If such a system is operative in B. ruminicola, then adenosine 5'-triphosphate (ATP) yields will be higher than can be accounted for by substrate-level phosphorylation reactions. Caldwell (D. R. Caldwell, Diss. Abstr. Int. B. 31:1419) has previously suggested that ATP yields in B. ruminicola strain GA33 are between 4.5 and 7.8 ATP/ mol of glucose, based on measurements of cellular protein. MATERIALS AND METHODS Bacteria. B. ruminicola subsp. brevis, strain B,4 (7, 8), was used in the present work and was kindly supplied by M. P. Bryant of the University of Illinois. This strain was used in preference to the type strain, strain GA33, because strain B,4 clumps rather less ...

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“…Succinogenic bacteria have many pathways to pursue due to the wide distribution of succinyl coenzyme A in amino acid and carbohydrate metabolism. Additionally, "4CO2 incorporation into succinate has been reported during carbohydrate fermentation by rumen Bacteroides (1).…”

Section: Discussionmentioning

confidence: 98%

Effects of Time and Growth Media on Short-Chain Fatty Acid Production by Bacteroides fragilis

Mayhew¹,

Onderdonk²,

Gorbach³

1975

Applied Microbiology

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Gas-liquid chromatography was used to monitor the evolution of short chain fatty acids by Bacteroides fragilis in five media. Acetic and succinic acids, the prominent end products encountered, were readily detected within 24 h. Propionic, isobutyric, butyric, isovaleric, and lactic acids were usually recorded in more limited quantities. Maximum rates of bacterial multiplication, glucose catabolism, and end-product production coincided with the first 24 h in carbohydrate-supplemented media. Extended incubation (672 h) favored substantial succinate increases in three of five media. These observations suggest that incubation time and composition of the medium are important determinants in short chain fatty acid production by B. fragilis.

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Effects of Carbon Dioxide on Growth and Maltose Fermentation by Bacteroides amylophilus

Abstract: To confirm that these strains were, in fact, B. amylophilus, the strains were characterized by use of previously described presumptive identification procedures (6, 668

Cited by 29 publications

References 33 publications

Effect of Glucose and Low Oxygen Tension on l-Asparaginase Production by a Strain of Escherichia coli B

Effect of Glucose and Low Oxygen Tension on l-Asparaginase Production by a Strain of Escherichia coli B

Metabolism and growth yields in Bacteroides ruminicola strain b14

Effects of Time and Growth Media on Short-Chain Fatty Acid Production by Bacteroides fragilis

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