SUMMARYUsing near-infrared spectroscopy, we investigated the time-course of the concentrations of oxygenated haemoglobin, [oxy-Hb], and deoxygenated haemoglobin, [deoxy-Hb], in the occipital cortex of healthy human adults during sustained visual stimulation. Within a few seconds after stimulation (by coloured dodecahedron), we observed a decrease in [deoxy-Hb], peaking after 13 s (' initial undershoot '). In the subsequent 1-2 min, in seven out of ten subjects, [deoxy-Hb] gradually returned to a plateau closer to the baseline level. After cessation of stimulation, there was a ' post-stimulus overshoot ' in [deoxy-Hb]. There was a statistically significant correlation between the size of the ' initial undershoot ' and the ' post-stimulus overshoot '.The concentration of oxyhaemoglobin increased upon functional activation. However, in the mean across all subjects there was no ' initial overshoot '. After approximately 19 s it reached a plateau and remained constantly elevated throughout the activation period. After cessation of activation there was a ' post-stimulus undershoot ' of oxyhaemoglobin.It is important to consider the time-course of haemoglobin oxygenation when interpreting functional activation data, especially those data obtained with oxygenation-sensitive methods, such as BOLDcontrast fMRI.