Effects of Carvacrol on Sister Chromatid Exchanges in Human Lymphocyte Cultures

Ipek, Evrim; Tüylü, Berrin Ayaz; Zeytinoğlu, Hülya

doi:10.1023/b:cyto.0000039896.26707.40

Cited by 52 publications

(29 citation statements)

References 17 publications

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“…A marked dose-dependent differential toxicity was observed with carvone and, to a lesser extent, with cinnamaldehyde in the DNA repair test while with thymol and carvacrol this effect was less pronounced. A set of cytogenetic analyses of human blood peripheral lymphocytes treated with carvacrol were carried out by Ipek et al [54] using the in vitro sister chromatid exchange (SCE) assay. According to the data obtained carvacrol did not increase the formation of SCE, however it inhibited the rate of SCE induced by mitomycin C and acted as an antigenotoxic agent.…”

Section: Mutagenicity Anti-mutagenicity and Dna-protective Effectsmentioning

confidence: 99%

Cytotoxic, anti-carcinogenic and antioxidant properties of the most frequent plant volatiles

Slameñová¹,

Horváthová²

2013

neo

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Flowers, berries, leaves, barks and roots of different plants have been used through the ages as a source of flavor in food and perfume preparations. The volatiles responsible for the flavor of botanicals can be extracted from the plant material as "essential oils" (EOs), called also volatile oils or ethereal oils. The term essential is intended to indicate that the oil is the fragrant essence of the plant from which it is extracted. EOs are constituted by hydrocarbons (monoterpenes and sesquiterpenes) and oxygenated compounds (alcohols, esters, ethers, aldehydes, ketones, lactones, phenols and phenol ethers). Of the numerous groups of naturally occurring compounds examined so far terpenes are known as fragrances and flavoring agents. The data reported in this review including the data obtained in our laboratory show that many of EOs exhibit a range of biological activities inclusive of antioxidative, anti-mutagenic and anti-carcinogenic activities. Most of them belong to phytochemicals with chemopreventive potential. On the other hand some herbal products can cause serious adverse effects. A complex research of toxic, genotoxic, anti-mutagenic and anti-carcinogenic effects of EOs is therefore very important.

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Section: Mutagenicity Anti-mutagenicity and Dna-protective Effectsmentioning

confidence: 99%

Cytotoxic, anti-carcinogenic and antioxidant properties of the most frequent plant volatiles

Slameñová¹,

Horváthová²

2013

neo

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“…It was confirmed by a set of experiments that at the doses above 10 μl/ml, carvacrol showed toxic effect against lymphocytes 9 .…”

Section: Resultsmentioning

confidence: 68%

Essential Oil Compositions ofThymus kotschyanusBoiss. Obtained by Hydrodistillation and Microwave Oven Distillation

Khanavi

Hajimehdipoor

Emadi

et al. 2013

Journal of Essential Oil Bearing Plants

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Abstract:The essential oils of the aerial parts of Thymus kotschyanus Boiss. obtained by hydrodistillation (HD) and microwave oven distillation (MD) were analyzed by GC and GC-MS. 39 components were identified in the essential oil of this plant (95.0 % in HD and 94.8 % in MD). The components of the essential oil extracted by MD were similar to those obtained by HD. T. kotschyanus essential oil was characterized by a high amount of carvacrol (64.6 % in HD and 44.7 % in MD). Other major compounds were identified as p-cymene, γ-terpinene and L-Borneol. Thymoquinone was absent in HD method. Although the time of MD was 12 times less than HD, but the percentage of essential oil was only 15 % less (1.2 % in MD and 1.4 % in HD). Moreover, the amount of carvacrol as the main component of the plant essential oil, in MD was 20 % less than HD.

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“…Thymus extract did not increase the mean number of SCEs significantly at three concentrations, however, the highest concentration increased this number. Previous reports showed that carvacrol and thymol, components of TE, significantly increased DNA damage at high concentration levels ( _ Ipek et al 2003;Aydın et al 2005). In the microbial and mammalian short-term assays, thymol and carvacrol showed very weak genotoxic potential, however, the possibility of their action at DNA level cannot be excluded because, at least carvacrol caused nuclear fragmentation (Stammati et al 1999).…”

Section: Discussionmentioning

confidence: 94%

“…_ Ipek et al (2003) reported that carvacrol strongly reduced MMCinduced SCE frequencies in a dose dependent manner. The differences between our results and _ Ipek et al (2003)'s may be due to different concentrations used or direct application of plant extract instead of pure carvacrol. In the present study, MMC + all concentrations of TE significantly decreased MI compared with negative control.…”

Section: Discussionmentioning

confidence: 97%

“…Recent investigations suggest that thyme extracts are antimutagenic (Natake et al 1989) and that luteolin, a constituent of thyme, is a strong antimutagen against the dietary carcinogen Trp-P-2 (3-amino-1-methyl-gamma-carboline) (Samejima et al 1995). _ Ipek et al (2003) reported that carvacrol did not increase the formation of SCE, in contrast, it inhibited the rate of SCE induced by Mitomycin-C (MMC). In another study major constituent of thyme, i.e thymol and c-terpinene did not seem to induce DNA strand breakage at lower concentrations in human lymphocytes, but at higher concentrations, they caused DNA damage (Aydın et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Thymus kotschyanus var. glabrescens Boiss. extract on mitomycin-C induced chromosomal aberrations and sister chromatid exchanges in human lymphocytes

et al. 2006

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In this investigation, clastogenic effects of Thymus kotschyanus var. glabrescens Boiss. extract (TE) and anticlastogenic effects of this extract against Mitomycin C (MMC) induced chromosome damage have been evaluated in human peripheral blood lymphocytes in vitro. Two series of experiments were conducted. In the first, only 10 -5 , 10 -4 , 10 -3 and 10 -2 ll ml -1 concentrations of TE were used for 48 h to detect potential clastogenicity. In the second, MMC (0.38 lg ml -1 ) plus 10 -5 , 10 -4 , 10 -3 and 10 -2 ll ml -1 concentrations of TE were used for 48 h to determine anticlastogenic effects. TE did not increase sister chromatid exchanges (SCEs) (except 10 -2 ll ml concentration) and chromosome aberrations (CAs) significantly compared with negative and solvent controls. However, it decreased the frequency of MMC induced chromosome aberrations. Decreasing was significant at 10 -4 , 10 -3 and 10 -2 ll ml -1 concentrations. On the other hand, TE significantly increased MMC-induced SCEs for all treatment groups compared with positive control.

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Effects of Carvacrol on Sister Chromatid Exchanges in Human Lymphocyte Cultures

Cited by 52 publications

References 17 publications

Cytotoxic, anti-carcinogenic and antioxidant properties of the most frequent plant volatiles

Cytotoxic, anti-carcinogenic and antioxidant properties of the most frequent plant volatiles

Essential Oil Compositions ofThymus kotschyanusBoiss. Obtained by Hydrodistillation and Microwave Oven Distillation

Effects of Thymus kotschyanus var. glabrescens Boiss. extract on mitomycin-C induced chromosomal aberrations and sister chromatid exchanges in human lymphocytes

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