Effects of castration-induced visceral obesity and antioxidant treatment on lipid profile and insulin sensitivity in New Zealand white rabbits

Georgiev, Ivan; Georgieva, T. M.; Иванов, Веселин; Dimitrova, Sylviya; Kanelov, I.; Vlaykova, Tatyana; Tanev, Stoyan; Zaprianova, Dimitrinka; Dichlianova, Еvgenia; Penchev, G.; Lazarov, L; Vachkova, E.; Roussenov, A.

doi:10.1016/j.rvsc.2010.05.023

Cited by 28 publications

(25 citation statements)

References 49 publications

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“…Lately, the New Zealand rabbit is a preferred animal model, widely used to study visceral obesity, atherosclerosis (Yanni, 2004), metabolic syndrome and insulin resistance (Mitsuguchi et al, 2008;Zhao et al, 2008;Georgiev et al, 2011;Ivanova et al, 2015;Niimi et al, 2016). In rabbits, not only the visceral, but also the subcutaneous fat is formed in depots (Cinti, 2005).…”

Section: Discussionmentioning

confidence: 99%

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Fatty acid composition of subcutaneous and visceral fat depots in New Zealand white rabbits

Yonkova¹,

Mihaylova²,

Ribarski³

et al. 2017

BJVM

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Section: Discussionmentioning

confidence: 99%

“…Therefore, specific fatty acid profiles of each fat depot should be taken into account when using the rabbit as an animal model of central obesity (Caroll et al, 1996;Zhao et al, 2008;Georgiev et al, 2011;Ivanova et al, 2015;Niimi et al, 2016). For this reason, the omental depot was included in this study.…”

Section: Discussionmentioning

confidence: 99%

Fatty acid composition of subcutaneous and visceral fat depots in New Zealand white rabbits

Yonkova¹,

Mihaylova²,

Ribarski³

et al. 2017

BJVM

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“…The design of the experiment has already been reported in details (Georgiev et al, 2011). Briefly, 2-2.5 month-old rabbits were randomly divided into 3 groups: i) first group (CIm; n=6) -castrated obese and treated with Immunoprotect for 2 months; ii) second group (CO; n=6) -castrated obese; and iii) third group (NC; n=7) -control group, non-castrated non-obese.…”

Section: Experimental Design and Experimental Proceduresmentioning

confidence: 99%

“…Recently, our laboratory created an animal model, using castrated male New Zealand white rabbits and reported that 2 months after castration rabbits developed visceral obesity, dyslipidaemia and impaired glucose tolerance (Georgiev et al, 2011). Antioxidant (vitamin E and d-limonene) treatments affected favourably blood lipid profile and glucose kinetics (Georgiev et al, 2009;.…”

Section: Introductionmentioning

confidence: 99%

Effect of antioxidant treatment on some indicators of obesity-induced changes in insulin sensitivity and beta-cell function in New Zealand white rabbits

Ivanova¹,

Penchev²,

Ribarski³

et al. 2015

BJVM

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I. Penchev Georgiev, 2015. Effect of antioxidant treatment on some indicators of obesity-induced changes in insulin sensitivity and beta-cell function in New Zealand white rabbits. Bulg. J. Vet. Med., 18, No 3,[194][195][196][197][198][199][200][201][202][203][204][205][206][207][208] The current study was conducted to investigate the impact of dietary antioxidant supplementation on obesity-induced changes in some surrogate indices of insulin sensitivity and ß-cell function in New Zealand white rabbits. Three groups of rabbits were used in this experiment: castrated animals treated with antioxidants (vitamin E and d-limonene, Immunoprotect) (Cim; n=6), castrated obese animals (CO; n=6) and non-castrated non-obese controls (NC; n=7). At the end of the follow-up period of 2 months after castration an intravenous glucose tolerance test (IVGTT) was performed after 12-hour fasting. Blood samples for determination of simplified estimates of insulin resistance and β-cell function were obtained at baseline and at various time intervals over the 120-min test. In addition, lipid content in m. Longissimus lumborum и m. Semimembranosus was determined. Some of the simplified measurements of insulin resistance (fasting insulin, fasting insulin to glucose ratio, НОМA ins.resist index), beta-cell function (HOMA β-cell , AUC insulin 0→60 min ) and muscles lipid content in CO were higher while QUICKI and Bennett indices were lower than in controls. No differences in surrogate indices between CIm and NC groups were found suggesting improvement of insulin sensitivity and β-cell function after antioxidant supplementation. Surrogate indices are simple and reliable indicators of insulin sensitivity and β-cell function in rabbits as they were closely associated with markers of obesity and can be modified by antioxidant supplementation.

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“…Rabbits are considered as an appropriate species in development of animal models for atherosclerosis and insulin resistance studies, because of their similarity to human blood lipid profile (Aguilera et al 2002;Yanni 2004). Various high fat diet and interventions (as castration, for example) have been used in rabbits for induction of metabolic syndrome (MS) and its consequence disorders such as insulin resistance, visceral obesity, hypertension, and dyslipidemia (Georgiev et al 2011;Vignozzi et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Adipogenic potential of stem cells derived from rabbit subcutaneous and visceral adipose tissue in vitro

Vachkova

Bosnakovski

Yonkova

et al. 2016

In Vitro Cell.Dev.Biol.-Animal

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Rabbits are considered as appropriate animal models to study some obesity-associated abnormalities because of the similarity of their blood lipid profile and metabolism to humans. The current study was focused on comparison of adipose differentiation ability in rabbit adipose-derived stem cells (ADSC) in vitro. Subcutaneous and visceral stromal vascular fractions (SVF) were isolated from three 28-d-old New Zealand rabbits by collagenase digestion. Supernatants from both isolates were collected 24 h after the initial plating. On the fourth passage, all isolated cell types undergo triplicate adipogenic induction. The adipose induction potential was calculated as percentage of increasing optical density (OD) values. The data revealed that with increasing the number of induction cycles, the induction tendency in visceral ADSC decreased in contrast to the subcutaneous ones. Although the supernatants did not reach induction levels of their relevant precursors, they follow the same pattern in both subcutaneous and visceral ADSC. All cell types successfully passed osteogenic and chondrogenic differentiation. In conclusion, the best adipose induction ability was observed in directly plated subcutaneous cell population. The increase of induction numbers depressed adipose induction ability in cell populations derived from visceral fat depots.

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Effects of castration-induced visceral obesity and antioxidant treatment on lipid profile and insulin sensitivity in New Zealand white rabbits

Cited by 28 publications

References 49 publications

Fatty acid composition of subcutaneous and visceral fat depots in New Zealand white rabbits

Fatty acid composition of subcutaneous and visceral fat depots in New Zealand white rabbits

Effect of antioxidant treatment on some indicators of obesity-induced changes in insulin sensitivity and beta-cell function in New Zealand white rabbits

Adipogenic potential of stem cells derived from rabbit subcutaneous and visceral adipose tissue in vitro

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