Effects of Celery Seed Extracts on Some Haematological and Biochemical Parameters in Albino Rats Treated with Gentamicin

Abstract

The present study targeted the influence of orally celery seeds (Apium graveolens) aqueous extract and ethanolic extracts on male albino rats injected with gentamicin intraperitoneally to investigate some hematological and biochemical parameters. Thirty-two male rats were weighing 300-400 gm carried out for the present research, they controlled environmentally. Animals were divided equally and randomly into four groups each of which contained eight rats. First group (control) included normal rats, group2 were given 100 mg/kg B.W. gentamicin (GM) intraperitoneally (IP), group3 (combined group) were given 100 mg/kg B.W. GM and 150 mg/kg B.W. celery seed ethanolic extract orally by needle gavage, and group4 (combined group) were given 100 mg/kg B.W. GM (IP) and celery seeds aqueous extract 150 mg/kg B.W. orally by needle gavage respectively the present study contiued for eight executive days. The results showed that the elevated by GM haematological parameters lowered by the celery seed aqueous extract as compared to the celery seed ethanolic extract group, while the elevated serum malondialdehyde (MDA) and total cholesterol (TC) are decreased in group3 more than they were in the group4, in contrast to that the triglyceride (TG), high density lipoprotein (HDL), low-density lipoprotein (LDL), serum creatinine (CR), serum uric acid (UA), and alkaline phosphatase (ALP) lowered in the group4 from the group 3. The present study concluded that both extracts of celery seeds play a vital biological role, including the improvement effects against the side effect of GM and offering health benefits through decreasing of elevated parameters. Preparations of laboratory animalsMale albino rats (Rattus norvegicus) were purchased from animal house (Zakho University-Iraq) and they were housed in animal house (Biology department of Faculty of science, Soran University, Iraq). The present study was performed thirty-two male albino rats. Animals were weighting about 300 -400 gm. They acclimatized in an environmentally controlled room at constant temperature 22 ± 2 •C on a lighting schedule 12 h light and 12 h darkness, they were maintained at free access to tap water ad libitum they fed at a standard pelleted feed according to Pico Lab. During the experiment the cages were cleaned once a week. Preparation of the dietThe constituents of diet were determined according Pico Lab. Rodent diet 20, with assistance of expert of in Erbil poultry project and Erbil animal diet factory, Iraq, as following; wheat 66.6%, soya 25.6%, oil sun flower 4.4%, lime stone 1.5%, salt 0.63%, methionine 0.156%, lysine 0.244%, choline chloride 0.062%, vit CX lay 0.058%, dicalcium phosphate 0.642%, AZ /1200= 0.080% and trace elements 0.050%. Preparation gentamicin doseGentamicin ampules were purchased in the local pharmaceutical were the patients used them as an antibiotics against disease related with respiratory system, the dose of 80 mg/kg B.W. was determined and injected intraperitoneally (IP) daily for eight executive days. Preparation of celery seeds extra...

Comparative evaluation of antioxidant characteristics and selenium levels in seeds of Cichorium intybus L. and several representatives of Apiaceae plants

“…Семена сельдерея применяют также для лечения артрита, снижают уровень холестерина, замедляют развитие рака легких и рака желудка, приводя к апоптозу раковых клеток [10,11]. Семена проявляют антиоксидантные, противовоспалительные и анальгетические свойства [12].…”