Effects of cell cycle phases on the induction of dental pulp stem cells toward dopaminergic‐like cells

Gnanasegaran, Nareshwaran; Govindasamy, Vijayendran; Kathirvaloo, Premasangery; Musa, Sabri; Kasim, Noor Hayaty Abu

doi:10.1002/term.2401

Cited by 13 publications

(7 citation statements)

References 36 publications

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“…1). This further confirms the applicability of DPSCs as a suitable cell source for CRT especially in PD cases (49). Although the fact that apoptosis can also invariably take place in this situation is undeniable, our study did not look into this aspect as one of vital parameter.…”

Section: Discussionsupporting

confidence: 69%

Neuroimmunomodulatory properties of DPSCs in an in vitro model of Parkinson's disease

et al. 2017

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In neurodegenerative diseases, such as Alzheimer's and Parkinson's, microglial cell activation is thought to contribute to their degeneration by producing neurotoxic compounds. While dental pulp stem cells (DPSCs) have been regarded as the next possible cell source for cell replacement therapy (CRT), their actual role when exposed in such harsh environment remains elusive. In this study, the immunomodulatory behavior of DPSCs from human subjects was investigated in a coculture system consisting of neuron and microglia which were treated with 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine, which mimics the inflammatory conditions and contribute to degeneration of dopaminergic (DA-ergic) neurons. Assessments were performed on their proliferation, extent of DNA damage, productions of reactive oxygen species (ROS) and nitric oxide (NO), as well as secretion of inflammatory mediators. Notably, DPSCs were shown to attenuate their proliferation, production of ROS, and NO significantly (P < 0.05). Additionally, their immunomodulatory properties were distinct although insignificant changes were observed in DNA damage. Despite DPSCs were exposed to such harsh environment, they were still able to express neuronal markers such as Nestin, Pax 6, and Nurr1, at least by twofold thereby indicating their applicability for CRT especially in PD conditions. To conclude, DPSCs were shown to have immunomodulatory capacities which could probably serve as secondary effects upon transplantation in a CRT regime. © 2017 IUBMB Life, 69(9):689-699, 2017.

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Section: Discussionsupporting

confidence: 69%

Neuroimmunomodulatory properties of DPSCs in an in vitro model of Parkinson's disease

et al. 2017

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“…At 24 h time point post‐incubation with various LPS concentration, the release of cytokines were assessed via ELISA as described earlier (Gnanasegaran et al, ). From the collected supernatant, presence of IL‐6, IL‐8, IL‐1α and TNF α was detected using Tecan micro plate reader (Infinite 200 PRO Tecan, Life sciences, Switzerland) with test wavelength adjusted at 450 nm and reference wavelength at 650 nm.…”

Section: Methodsmentioning

confidence: 99%

Immune responses of human dental pulp stem cells in lipopolysaccharide‐induced microenvironment

Bindal

Ramasamy

Kasim

et al. 2018

Cell Biology International

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This study aimed to investigate the effect of inflammatory stimuli on dental pulp stem cells (DPSCs) by assessing their proliferation and expression of genes as well as proteins in lipopolysaccharide (LPS)-induced microenvironment (iDPSCs). DPSCs were first characterized for their mesenchymal properties prior to challenging them with a series of LPS concentrations from 12 to 72 h. Following to this, their proliferation and inflammatory based genes as well as protein expression were assessed. iDPSCs had demonstrated significant expression of mesenchymal markers. Upon exposure to LPS, the viability dropped distinctly with increasing concentration, as compared to control (P < 0.05). The expression of pro-inflammatory genes such as interleukin 6, interleukin 8 were augmented with exposure to LPS (P < 0.05). Similarly, cytokines like tumour necrosis factor (TNF) α and interleukin 1α had increased in dose dependant manner upon LPS exposure (P < 0.05). Our results suggest that LPS concentration between 1 and 2 μg/mL demonstrated inflammation induction in DPSCs that may simulate inflamed microenvironment of dental pulp in clinical scenario. Thus, optimizing iDPSCs secretome profile could be a promising approach to test various regenerative protocols in inflamed microenvironment.

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“…Studies of Chang et al reported that DPSCs could be directly differentiated into motor neurons by growth factors and small molecules, for example, BDNF and all-trans retinoic acid [ 71 ]. Gnanasegaran et al demonstrated that DPSCs could be induced to differentiate into dopaminergic-like cells by multistage inductive protocols [ 72 ]. The study of Singh et al showed that DPSCs are induced by a two-step method to generate functional dopaminergic neurons: FGF2 first with an addition of BDNF on 9th day.…”

Section: Dental Pulp Stem Cells (Dpscs)mentioning

confidence: 99%

Potential Roles of Dental Pulp Stem Cells in Neural Regeneration and Repair

Luo

Wang

et al. 2018

Stem Cells International

126

143

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This review summarizes current advances in dental pulp stem cells (DPSCs) and their potential applications in the nervous diseases. Injured adult mammalian nervous system has a limited regenerative capacity due to an insufficient pool of precursor cells in both central and peripheral nervous systems. Nerve growth is also constrained by inhibitory factors (associated with central myelin) and barrier tissues (glial scarring). Stem cells, possessing the capacity of self-renewal and multicellular differentiation, promise new therapeutic strategies for overcoming these impediments to neural regeneration. Dental pulp stem cells (DPSCs) derive from a cranial neural crest lineage, retain a remarkable potential for neuronal differentiation, and additionally express multiple factors that are suitable for neuronal and axonal regeneration. DPSCs can also express immunomodulatory factors that stimulate formation of blood vessels and enhance regeneration and repair of injured nerve. These unique properties together with their ready accessibility make DPSCs an attractive cell source for tissue engineering in injured and diseased nervous systems. In this review, we interrogate the neuronal differentiation potential as well as the neuroprotective, neurotrophic, angiogenic, and immunomodulatory properties of DPSCs and its application in the injured nervous system. Taken together, DPSCs are an ideal stem cell resource for therapeutic approaches to neural repair and regeneration in nerve diseases.

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Effects of cell cycle phases on the induction of dental pulp stem cells toward dopaminergic‐like cells

Cited by 13 publications

References 36 publications

Neuroimmunomodulatory properties of DPSCs in an in vitro model of Parkinson's disease

Neuroimmunomodulatory properties of DPSCs in an in vitro model of Parkinson's disease

Immune responses of human dental pulp stem cells in lipopolysaccharide‐induced microenvironment

Potential Roles of Dental Pulp Stem Cells in Neural Regeneration and Repair

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