Effects of cell density and temperature on oxygen consumption rate for different mammalian cell lines

Jorjani, P.; Öztürk, Sadettin S.

doi:10.1002/(sici)1097-0290(19990805)64:3<349::aid-bit11>3.0.co;2-v

Cited by 91 publications

(58 citation statements)

References 15 publications

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“…There have been some measurements of the effects of temperature on aerobic metabolism at the cellular level in animals (Somero and DeVries, 1967;Hoskins and Aleksiuk, 1973;Jorjani and Ozturk, 1999) and some data sets conform well to the Arrhenius equation and others do not. Similar patterns occur for measurements of oxygen consumption by isolated mitochondria (van den Thillart and Modderkolk, 1978;Somero et al, 1996;Weinstein and Somero, 1998;Hardewig et al, 1999;Abele et al, 2002;Somero, 2002;Johnston et al, 1994;Fangue et al, 2009;Hilton et al, 2010;Iftikar et al, 2010Iftikar et al, , 2014Iftikar and Hickey, 2013).…”

Section: Effects Of Temperature At the Level Of Single Proteinsmentioning

confidence: 99%

The effects of temperature on aerobic metabolism: towards a mechanistic understanding of the responses of ectotherms to a changing environment

Schulte¹

2015

Journal of Experimental Biology

631

474

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Because of its profound effects on the rates of biological processes such as aerobic metabolism, environmental temperature plays an important role in shaping the distribution and abundance of species. As temperature increases, the rate of metabolism increases and then rapidly declines at higher temperatures -a response that can be described using a thermal performance curve (TPC). Although the shape of the TPC for aerobic metabolism is often attributed to the competing effects of thermodynamics, which can be described using the Arrhenius equation, and the effects of temperature on protein stability, this account represents an over-simplification of the factors acting even at the level of single proteins. In addition, it cannot adequately account for the effects of temperature on complex multistep processes, such as aerobic metabolism, that rely on mechanisms acting across multiple levels of biological organization. The purpose of this review is to explore our current understanding of the factors that shape the TPC for aerobic metabolism in response to acute changes in temperature, and to highlight areas where this understanding is weak or insufficient. Developing a more strongly grounded mechanistic model to account for the shape of the TPC for aerobic metabolism is crucial because these TPCs are the foundation of several recent attempts to predict the responses of species to climate change, including the metabolic theory of ecology and the hypothesis of oxygen and capacity-limited thermal tolerance.

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Section: Effects Of Temperature At the Level Of Single Proteinsmentioning

confidence: 99%

The effects of temperature on aerobic metabolism: towards a mechanistic understanding of the responses of ectotherms to a changing environment

Schulte¹

2015

Journal of Experimental Biology

631

474

View full text Add to dashboard Cite

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“…The mechanism whereby cells at lower temperatures improve productivity is still poorly understood although recent studies have demonstrated that reduced culture temperature invokes a coordi- nated response involving the cell cycle, transcription and translational machinery, and the arrangement of the cytoskeleton (Chuppa et al 1997;Moore et al 1997;Ohsuye 1998, 1999;Jorjani and Ozturk 1999;Hendrick et al 2001;Yoon et al 2003b;Fox et al 2004Fox et al , 2005Baik et al 2006;Al-Fageeh et al 2006). Low culture temperature results in reduced metabolism (glucose/medium consumption, oxygen uptake, lactate production and ammonia production) and shear sensitivity which leads to delayed initiation of apoptosis and helps in extending the duration of the stationary/production phase (Moore et al 1997;Chuppa et al 1997;Ohsuye 1998, 1999;Jorjani and Ozturk 1999;Hendrick et al 2001;Yoon et al 2003a, b;Trummer et al 2006;Wong et al 2006). Reduced culture temperature has also been shown to increase levels of recombinant mRNA, either due to enhanced transcription of the recombinant gene of interest or increased mRNA stability (Hendrick et al 2001;Yoon et al 2003b;Fox et al 2004Fox et al , 2005.…”

Section: The Use Of Cell Cycle Arrest To Increase Recombinant Proteinmentioning

confidence: 99%

Proliferation control strategies to improve productivity and survival during CHO based production culture

2007

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Chinese Hamster Ovary cells are the primary system for the production of recombinant proteins for therapeutic use. Protein productivity is directly proportional to viable biomass, viability and culture longevity of the producer cells and a number of approaches have been taken to optimise these parameters. Cell cycle arrest, particularly in G1 phase, typically using reduced temperature cultivation and nutritional control have been used to enhance productivity in production cultures by prolonging the production phase, but the mechanism by which these approaches work is still not fully understood. In this article, we analyse the public literature on proliferation control approaches as they apply to production cell lines with particular reference to what is known about the mechanisms behind each approach.

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“…22 A decrease in the microwell diameter results in a decrease in the number of attached cells within the microwells. Figure 7 shows the dependence of the OCR (pmol min 23,24 This result is significant and suggests that our proposed system can be used to compare single-cell respiration rates to a large number of single cells in multiple arrays.…”

Section: Correlation Of the Ocr With Cell Numbermentioning

confidence: 92%

Light-addressable measurements of cellular oxygen consumption rates in microwell arrays based on phase-based phosphorescence lifetime detection

Huang

Hsu

et al. 2012

Biomicrofluidics

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A digital light modulation system that utilizes a modified commercial digital micromirror device (DMD) projector, which is equipped with a UV light-emitting diode as a light modulation source, has been developed to spatially direct excited light toward a microwell array device to detect the oxygen consumption rate (OCR) of single cells via phase-based phosphorescence lifetime detection. The microwell array device is composed of a combination of two components: an array of glass microwells containing Pt(II) octaethylporphine (PtOEP) as the oxygensensitive luminescent layer and a microfluidic module with pneumatically actuated glass lids set above the microwells to controllably seal the microwells of interest. By controlling the illumination pattern on the DMD, the modulated excitation light can be spatially projected to only excite the sealed microwell for cellular OCR measurements. The OCR of baby hamster kidney-21 fibroblast cells cultivated on the PtOEP layer within a sealed microwell has been successfully measured at 104 6 2.96 amol s À1 cell À1. Repeatable and consistent measurements indicate that the oxygen measurements did not adversely affect the physiological state of the measured cells. The OCR of the cells exhibited a good linear relationship with the diameter of the microwells, ranging from 400 to 1000 lm and containing approximately 480 to 1200 cells within a microwell. In addition, the OCR variation of single cells in situ infected by Dengue virus with a different multiplicity of infection was also successfully measured in real-time. This proposed platform provides the potential for a wide range of biological applications in cell-based biosensing, toxicology, and drug discovery. V C 2012 American Institute of Physics.

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Effects of cell density and temperature on oxygen consumption rate for different mammalian cell lines

Cited by 91 publications

References 15 publications

The effects of temperature on aerobic metabolism: towards a mechanistic understanding of the responses of ectotherms to a changing environment

The effects of temperature on aerobic metabolism: towards a mechanistic understanding of the responses of ectotherms to a changing environment

Proliferation control strategies to improve productivity and survival during CHO based production culture

Light-addressable measurements of cellular oxygen consumption rates in microwell arrays based on phase-based phosphorescence lifetime detection

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