Effects of challenge dose on the clinical and immune responses of cattle vaccinated with reduced doses of Brucella abortus strain 19

Confer, Anthony W.; Hall, Shelagh M.; Faulkner, C.B.; Espe, Brian H.; Deyoe, B L; Morton, Robert; Smith, Richard A.

doi:10.1016/0378-1135(85)90065-3

Cited by 48 publications

(26 citation statements)

References 14 publications

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“…In comparison, an experimental challenge dose of 1.5 ϫ 10 7 CFU was the minimal dose for inducing 100% infection rates in naïve cattle (11). Others have reported that higher challenge doses may overwhelm protective immunity induced by vaccination (6). In the current study, the overall rate of abortion was slightly less than in a previous bison study, in which 96% of naive bison aborted following challenge with 10 7 CFU of B. abortus strain 2308 (7).…”

Section: Discussioncontrasting

confidence: 70%

Comparison of Abortion and Infection after Experimental Challenge of Pregnant Bison and Cattle with Brucella abortus Strain 2308

Olsen

Johnson

2011

Clin Vaccine Immunol

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A comparative study was conducted using data from naive bison (n ‫؍‬ 45) and cattle (n ‫؍‬ 46) from 8 and 6 studies, respectively, in which a standardized Brucella abortus strain 2308 experimental challenge was administered during midgestation. The incidence of abortion, fetal infection, uterine or mammary infection, or infection in maternal tissues after experimental challenge was greater (P < 0.05) in bison than in cattle. In animals that did abort, the time between experimental challenge and abortion was shorter (P < 0.05) for bison than for cattle. Brucella colonization of four target tissues and serologic responses on the standard tube agglutination test at the time of abortion did not differ (P > 0.05) between cattle and bison. The results of our study suggest that naive bison and cattle have similarities and differences after experimental exposure to a virulent B. abortus strain. Although our data suggest that bison may be more susceptible to infection with Brucella, some pathogenic characteristics of brucellosis were similar between bison and cattle.

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Section: Discussioncontrasting

confidence: 70%

Comparison of Abortion and Infection after Experimental Challenge of Pregnant Bison and Cattle with Brucella abortus Strain 2308

Olsen

Johnson

2011

Clin Vaccine Immunol

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“…4 Antigen preparations were V. villosa lectin at concentrations of 0.2, 1.0, and 2.0 µg/well. Pokeweed mitogen (0.4 µg/well) was used as the positive control; the negative control consisted of unstimulated cells.…”

Section: Experimental Production Of Diseasementioning

confidence: 99%

Hairy Vetch (Vicia VillosaRoth) Poisoning in Cattle: Update and Experimental Induction of Disease

1992

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Abstract. Hairy vetch poisoning (vetch-associated disease) of cattle is a generalized disease characterized pathologically by infiltration of skin and many internal organs by monocytes, lymphocytes, plasma cells, and often eosinophils and multinucleated giant cells and clinically by dermatitis, pruritis, often diarrhea, wasting, and high mortality. The disease was experimentally reproduced in an adult Angus female that had recovered from the natural disease 1 year earlier. She developed dermatitis on the 11th day of vetch feeding, and despite withdrawal from the vetch diet on the 12th day, death occurred 24 days after first day of vetch feeding. The cow developed lymphocytosis and hyperproteinemia. The results of other hematologic evaluations, blood chemical profiles, urinalysis, and cutaneous hypersensitivity tests using vetch lectin were normal. Lymphocyte blastogenesis studies with vetch lectin were not interpretable. Necropsy revealed gross lesions characteristic of the disease in the skin, heart, kidney, adrenal, and lymphoid tissues. Microscopically there was typical cellular infiltration in those organs and in the thyroid, liver, pancreas, salivary and mammary glands, urinary bladder, corpus luteum, and cerebral meninges. Cutaneous apocrine gland necrosis was present. The inflammatory reaction has qualities of a type-IV hypersensitivity reaction. Hypersensitivity may occur when constituents of the ingested plant are absorbed and act as antigens that sensitize lymphocytes and evoke the multisystemic granulomatous inflammatory response that characterizes the disease. Alternatively, vetch lectin may directly activate T lymphocytes to initiate the cellular response. Vetch-like diseases have been associated with a variety of diets that did not contain hairy vetch. The gross and histopathologic lesions of the vetch-associated and the vetch-like diseases are not mimicked in quality and distribution by any other disease.

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“…Diversos autores citam a proteção de bovinos à exposição a desafios de virulência, com aumento da resistência a abortos, alcançada com o uso de doses reduzidas da vacina B19 em animais adultos (Plommet & Fensterbank 1976, Nicoletti et al 1978, Nicoletti 1984, Confer et al 1985. A dose protetora mínima da vacina B19 está entre 10 10 e 10 7 UFC.…”

Section: Resul Resul Resul Resul Results T T T Tados Ados Ados Ados Adosunclassified

“…A dose protetora mínima da vacina B19 está entre 10 10 e 10 7 UFC. Apesar dos efeitos benéfi-cos do uso desse método de vacinação, títulos de anticorpos residuais induzidos por vacina, reações sorológicas e persistên-cia de infecção com a amostra podem ocorrer e causar reações falso-positivas nos testes de diagnóstico sorológico, e um pequeno aumento da dose de desafio pode resultar em aumento nas taxas de aborto (Confer et al 1985).…”

Section: Resul Resul Resul Resul Results T T T Tados Ados Ados Ados Adosunclassified

Diagnóstico sorológico da brucelose bovina em animais adultos vacinados com dose reduzida da cepa 19 de Brucella abortus

Jardim¹,

Pires²,

Mathias³

et al. 2006

Pesq. Vet. Bras.

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Com o presente trabalho avaliou-se o uso de dose reduzida da vacina produzida com a amostra 19 de Brucella abortus, em rebanho adulto negativo para a enfermidade, por meio de técnicas de diagnóstico sorológico preconizadas pelo Programa Nacional de Controle e Erradicação da Brucelose e Tuberculose Animal e por um ensaio indireto de imunoadsorção enzimática (ELISA ID). A prova de fixação de complemento detectou 46,77% de positivos, o antígeno acidificado tamponado 67,74%, o 2-mercaptoetanol com soroaglutinação lenta 87,09% e o ELISA ID 100%. A dose reduzida interferiu no diagnóstico sorológico. Nenhuma das técnicas apresentou especificidade adequada para uso em rebanho nestas condições, até 3 meses após a vacinação.

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Effects of challenge dose on the clinical and immune responses of cattle vaccinated with reduced doses of Brucella abortus strain 19

Cited by 48 publications

References 14 publications

Comparison of Abortion and Infection after Experimental Challenge of Pregnant Bison and Cattle with Brucella abortus Strain 2308

Comparison of Abortion and Infection after Experimental Challenge of Pregnant Bison and Cattle with Brucella abortus Strain 2308

Hairy Vetch (Vicia VillosaRoth) Poisoning in Cattle: Update and Experimental Induction of Disease

Diagnóstico sorológico da brucelose bovina em animais adultos vacinados com dose reduzida da cepa 19 de Brucella abortus

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