Effects of chelated complexes and probiotics on histological and morphometric parameters of the gastrointestinal tract of juvenile carp ( Cyprinus carpio )

Effects of Three Feed Additives on the Culturable Microbiota Composition and Histology of the Anterior and Posterior Intestines of Zebrafish (Danio rerio)

“…At concentrations of 1 and 2 mg kg −1 , there was an increase in the area of goblet cells in the anterior intestine and the presence of intraepithelial leukocytes in the posterior intestine. In our previous studies of the use of chelated compounds in low concentrations, similar histomorphological changes in the intestine (stimulation of immune cell migration into the mucosa, hypertrophy of goblet cells) were detected [ 61 , 62 ]. Perhaps such morphological changes in the intestines may be associated with lower toxicity and greater bioavailability of the chelated compounds.…”

“…Coppens vital 0.5–0.8 mm commercial pelleted feed (Coppens, Helmond, Netherlands) was used as the basal feed mixture. Butyric acid (BA) in concentrations (g kg −1 ) of 0.5, 1.0, and 2.0 [ 59 , 60 ] and organomineral chelated trace element compounds (CH) (Jupiter LLC, Tver, Russia) (composition of the mother liquor g L −1 : Fe—10; Mn—15; Zn—35; Se—0.3; I—1.1; Cu—3) in concentrations (g kg −1 ) of 0.5, 1.0, and 2.0 [ 61 , 62 , 63 ] were incorporated into the base feed by microencapsulation with sodium alginate. For this purpose, sodium alginate was dissolved in distilled water (0.5 mg 100 mL −1 ), and then the test additives in the described concentrations were added to the solution.…”