Effects of chemokine-like factor 1 on vascular smooth muscle cell migration and proliferation in vascular inflammation

Zhang, Tao; Zhang, Xiaoming; Yu, Weidong; Chen, Jian; Li, Qingle; Yang, Jicheng; He, Pei; Shen, Chenyang

doi:10.1016/j.atherosclerosis.2012.09.023

Cited by 23 publications

(23 citation statements)

References 21 publications

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“…Besides PDGF-BB, a second chemokine TNF-α was also used in this study since TNF-α is present in atherosclerotic plaques rather than in normal vessels 52 . In previous studies, we confirmed the positive role of TNF-α in regulating the migration of VSMCs 51 . Figure 5(a,d) summarizes the experimental results with quantified migration distances of 122.0 ± 12.8 μm (TNF-α plus, 25 μg/ml 51 ) vs. 108.1 ± 22.9 μm (FBS) (see Fig.…”

Section: Resultssupporting

confidence: 87%

“…PDGF-BB is the most potent chemoattractant for the migration of VSMCs, which has been assumed to play a major role in atherosclerosis and restenosis 11 . In the microfluidic would healing assay, a significant increase in migration distances of VSMCs due to PDGF-BB (10 μg/mL 51 ) was recorded as 247.7 ± 34.8 μm (PDGF-BB plus) vs. 108.1 ± 22.9 μm (FBS) ( Fig. 5(a,c,e) , p < 0.01).…”

Section: Resultsmentioning

confidence: 90%

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A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration

Wei

Chen

Zhang

et al. 2015

Sci Rep

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This paper presents a tubing-free microfluidic wound healing assay to quantify the migration of vascular smooth muscle cells (VSMCs), where gravity was used to generate a laminar flow within microfluidic channels, enabling cell seeding, culture, and wound generation. As the first systemic study to quantify the migration of VSMCs within microfluidic environments, the effects of channel geometries, surface modifications and chemokines on cellular migration were investigated, revealing that 1) height of the micro channels had a significant impact on cell migration; 2) the surface coating of collagen induced more migration of VSMCs than fibronectin coated surfaces and 3) platelet derived growth factor resulted in maximal cell migration compared to tumor necrosis factor alpha and fetal bovine serum. Furthermore, migrations of five types of VSMCs (e.g., the human vascular smooth muscle cell line, two types of primary vascular smooth cells, and VSMCs isolated from two human samples) were quantified, finding that VSMCs from the cell line and human samples demonstrated comparable migration distances, which were significantly lower than the migration distances of two primary cell types. As a platform technology, this wound healing assay may function as a new model to study migration of VSMCs within microfluidic environments.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 90%

A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration

Wei

Chen

Zhang

et al. 2015

Sci Rep

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“…Previous studies have verified that CKLFs have multiple activities, such as attract human neutrophils, lymphocytes, and monocytes and stimulate the proliferation and differentiation of murine skeletal myoblast cells. CKLF1 has shown broad chemotactic activities on leukocytes and plays essential roles in inflammatory and autoimmune diseases (12)(13)(14)(15)(16)(17). However, the relationship between CKLF1 and cancer is uncertain, particularly HCC.…”

Section: Introductionmentioning

confidence: 99%

CKLF1 Enhances Inflammation-Mediated Carcinogenesis and Prevents Doxorubicin-Induced Apoptosis via IL6/STAT3 Signaling in HCC

Liu

Zhou

et al. 2019

Clinical Cancer Research

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Purpose: Hepatocellular carcinoma (HCC), one of the most common and deadliest malignancies worldwide, has a poor prognosis, owing to its high potential for vascular invasion and metastasis and the lack of biomarkers for early diagnosis. Thus, it must be a crucial factor for investigating therapeutic strategies for HCC to identify the functional molecular targets. Here, we reported a novel chemokine, CKLF1, that might act as a pivotal modulator in the invasion and metastasis of HCC and could serve as an attractive target for cancer therapy.Experimental Design: Bioinformatics analysis, PCR, Western blotting, and IHC were performed to detect the expression of CKLF1 in HCC. The function of CKLF1 was demonstrated by a series of in vitro and in vivo experiments. Pharmacologic treatment, flow cytometry, and Western blotting were carried out to demonstrate the potential mechanisms of CKLF1.Results: We proved that CKLF1 was overexpressed in HCC tissues and was related to tumor stage, vascular invasion, and patient survival. Then, functional assays showed that CKLF1 promoted hepatocellular carcinogenesis and metastatic potential. Finally, the IL6/STAT3 signaling pathway was involved in the mechanistic investigation. The results demonstrated that CKLF1 enhanced the progression of HCC and prevented doxorubicin-induced apoptosis through activating the IL6/ STAT3 pathway.Conclusions: These data showed that CKLF1 inhibited apoptosis and promoted malignant transformation through the IL6/STAT3 pathway, and ultimately enhanced the development and metastasis of HCC. Thus, our work revealed that CKLF1 was a significant prognostic factor of HCC and might be a potential molecular therapeutic target for HCC.

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“…There is abundant evidences showing that the embryonic origin of the VSMCs plays an important role in vascular biology and in the response to the stimuli response [9,10]. Firstly, the different embryonic origins of VSMCs reflect different gene expression patterns [11]. Secondly, differentiation of VSMCs from embryonic stem cells through NC-or mesodermlineages showed that VSMC characteristics are programmed largely based on embryonic origin [12].…”

Section: Origin Of Vsmcsmentioning

confidence: 99%

Vascular Smooth Muscle Cell

Zhou¹,

Stoll²,

Leimena³

et al. 2018

Muscle Cell and Tissue - Current Status of Research Field

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Vascular smooth muscle cells (VSMCs) are the stromal cells of the vascular wall and are responsible for regulating arterial tone, blood pressure, and blood supply of the tissues. VSMCs display diversity in function and phenotype depending on their location within the arterial tree (large conduit vs. small resistance vessels), their embryologic origin, and their organ-dependent microenvironment. The heterogeneity of VSMCs is regulated by multiple mechanisms including intracellular signaling and changes in the VSMC microenvironment. Genetic disorders and extrinsic stimuli-induced dysfunction in VSMCs are associated with age-related vascular pathogenesis and vascular diseases, and thus are considered as a potential therapeutic target.

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Effects of chemokine-like factor 1 on vascular smooth muscle cell migration and proliferation in vascular inflammation

Cited by 23 publications

References 21 publications

A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration

A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration

CKLF1 Enhances Inflammation-Mediated Carcinogenesis and Prevents Doxorubicin-Induced Apoptosis via IL6/STAT3 Signaling in HCC

Vascular Smooth Muscle Cell

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