Effects of Chinese herbal medicines mixture on growth performance digestive enzyme activity immune response of juvenile Japanese seabass,Lateolabrax japonicus

Abstract

A single factorial experiment was conducted to investigate the effects of Chinese herbal medicines mixture (CHMM) on growth performance, digestive enzyme activity and immune response of Japanese seabass, Lateolabrax japonicus (initial weight 5.01 ± 0.32 g). The fish were fed diets containing six levels of CHMM (0, 4, 8, 12, 16 and 20 g/kg) for 4 weeks. The results showed that the weight gain rate and specific growth rate (SGR) enhanced significantly in fish fed diet containing 8 g/kg CHMM (p < .05), while the feed conversion ratio (FCR) in the 4 and 8 g/kg CHMM groups reduced significantly compared with the control (p < .05). The body crude protein levels in 8 and 16 g/kg groups were significantly higher than the control (p < .05). The pepsin, amylase and lipase activities elevated significantly in the stomach of fish fed 8 g/kg CHMM, while the erepsin and lipase in the intestine of fish in 12 and 16 g/kg groups were higher significantly than the control (p < .05). The lysozyme, alkaline phosphatase, acid phosphatase (ACP), total antioxidative capacity activities in serum of fish fed 12 g/kg CHMM were higher significantly than those in the control (p < .05), while the total superoxide dismutase (SOD), total antioxidative capacity, catalase, alkaline phosphatase (AKP) and ACP activities in hepatopancreas of fish in 12 g/kg group were all significantly higher than those in the control (p < .05). Regression analysis showed that the relationships between dietary CHMM levels and either FCR, SGR, erepsin, pepsin or lysozyme activities were best expressed by quadratic or cubic regression equations, and the optimal inclusion levels are 11.4, 10.7, 10.7, 8.4 and 10.5 g/kg for maximum FCR, SGR, erepsin, pepsin and lysozyme activities, respectively. Under the present experimental condition, the optimal supplementary level of CHMM in the diet of Japanese seabass is 8-12 g/kg. K E Y W O R D SChinese herbal medicines mixture, digestive enzyme activities, growth performance, immune response, Lateolabrax japonicus

Effects of garlic powder on feeding attraction activity, growth and digestive enzyme activities of Japanese seabass, Lateolabrax japonicus

“…Medicinal plants have been confirmed to promote aquatic animals growth performance, immunity and digestive enzyme activity (Abarike et al, ; Citarasu, ; Reverter, Bontemps, Lecchini, Banaigs, & Sasal, ; Wang et al, ). Several researchers have reported that garlic can improve growth performance of aquatic animals, such as sterlet sturgeon ( Acipenser ruthenus ), caspian roach ( Rutilus rutilus ), brown trout ( Salmo caspius ) and rainbow trout ( Oncorhynchus mykiss ) (Büyükdeveci, Balcazar, Demirkale, & Dikel, ; Ghehdarijani, Hajimoradloo, Ghorbani, & Roohi, ; Lee et al, ; Zaefarian, Yeganeh, & Adhami, ), and all of their results showed a similar trend garlic supplementation at 10 g/kg can significantly improve fish growth performance.…”

“…In the present study, the results showed garlic had a significant influence in the content of TP and activity of LPS and trypsin, and the highest values among all the groups were found at around 10 g/kg diet. Medicinal plants have functions on increasing fish intestinal digestion (Wang et al, ). Researches reported that garlic contains allicin, which can improve digestion and enhance the utilization of energy because of its beneficial effects on the intestinal microflora (Büyükdeveci et al, ; Lee et al, ).…”

“…At the end of the feeding trial, fish were fasted for 24 hr and then anaesthetized in eugenol (1:10,000) prior to weighing, dissection and blood sampling (Wang et al, ; Zhang et al, ). The growth performance was calculated as follows: Weight gain (WG, %) = ( W t − W 0 )/ W 0 × 100; Specific growth rate (SGR, %) = (ln W t − ln W 0 )/ t × 100; Feed conversion ratio (FCR) = W f /( W t − W 0 ); Feed intake (FI, %/day) = W f /[( W t + W 0 )/2]/ t × 100; Survival rate (SR, %) = ( N 0 − N t )/ N 0 × 100; Condition factor (K, g/cm 3 ) = W d / L 3 × 100; Hepatosomatic index (HSI, %) = ( W g / W d ) × 100. …”

Growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus fed with fructooligosaccharide

“…Serum lysozyme (LZM) activity was measured based on the lysis of Micrococcus luteus according to the method of Ellis (Wirth, Wolf, Wirth, & Wolf, ). The total protein (TP), alkaline phosphatase (AKP) and total superoxide dismutase (T‐SOD) activities in serum were determined using a reagent kit (Jiancheng) as described previously (Wang et al, ).…”

Effects of exogenous multienzyme complex supplementation in diets on growth performance, digestive enzyme activity and non‐specific immunity of the Japanese seabass,Lateolabrax japonicus

“…Japanese seabass were fed with the control diet for 2 weeks in circulating filter barrel, for them to adapt to the new diet and environmental conditions before starting the experiment. At the beginning of the experiment, the Japanese seabass were treated with feed deprivation for 24 hr, and weighed after being anaesthetized with eugenol (1:10,000) (Wang et al, ). 360 Japanese seabass with an average weight of (27.09 ± 0.08) g were selected and randomly assigned into six groups; each group repeated the experiment 3 times and 20 fishes were stocked each time.…”