Effects of chitosan nanoparticles, ivermectin and their combination in the treatment of Gasterophilus intestinalis (Diptera: Gasterophilidae) larvae in donkeys (Equus asinus)

AbdElKader, Naglaa A.; Sheta, Eldessouky; AbuBakr, Huda O.; El-Shamy, Omnia A. A.; Oryan, Ahmad; Attia, Marwa M.

doi:10.1007/s42690-020-00171-2

Cited by 23 publications

(9 citation statements)

References 35 publications

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“…Chitosan is a cationic polysaccharide derived naturally from chitin and chitosan as well as its derivatives have been used in a variety of fields because they are potent, inexpensive, simple to use, and suited for a variety of medical applications, such as wound dressings and bandages as they could enhance wound healing plus its antibacterial, antifungal and antiparasitic activities ( AbdElKader, et al, 2021 ; Attia et al, 2021 ). Recently, many parasites showed antiparasitic resistance against commonly used chemical antiparasitic preapations and as an alternative to chemically manufactured pesticides, the use of nanoparticles as a pesticide agent has become more common as technological advances have made their products more eco-friendly ( Alfy et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Incidence of gastrointestinal parasites in pigeons with an assessment of the nematocidal activity of chitosan nanoparticles against Ascaridia columbae

et al. 2022

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confidence: 99%

Incidence of gastrointestinal parasites in pigeons with an assessment of the nematocidal activity of chitosan nanoparticles against Ascaridia columbae

et al. 2022

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“…(2015) ; Attia and Salaeh (2020) ; then dehydrated through passing in ethanol series (50; 70; 90; 100%) and dried in CO2 critical point drier (Autosamdri-815, Germany). The adult was glued over stubs (as an anterior end and posterior end) and then; coated with 20 nm gold ( Abd ElKader et al., 2021 ) in a sputter coater (Spi-Module sputter Coater, UK). All specimens were photographed with a scanning electron microscope (JSM 5200, Electron prob); Microanalyzer Jeol, Japan; at Faculty of Agriculture, Cairo University, as described by Salem and Attia (2021) .…”

Section: Methodsmentioning

confidence: 99%

Morphological and molecular characterization of Ascaridia columbae in the domestic pigeon (Columba livia domestica) and the assessment of its immunological responses

et al. 2022

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Parasitism is a divesting problem that is frequently overlooked and may result in severe prominent clinical manifestation. This study aimed to investigate the seasonal and sexual prevalence of the gastrointestinal nematode Ascaridia columbae ( A. columbae ) infection among domestic pigeons in Giza governorate, Egypt, during the period from 2020 to 2021. One hundred and sixty suspected pigeons were clinically investigated. Blood & tissue samples were collected from infected birds to estimate serum zinc concentration, malondialdehyde ( MDA ), and nitric oxide levels. As well as tumor necrosis factor-alpha ( TNF-α ), interleukin 1β ( IL1β ) activity, and histopathological examination were estimated; also, worms were collected for morphological identification using electron microscope ( SEM ) and molecularly identified using polymerase chain reaction ( PCR ), further sequenced, and submitted in GenBank with accession number MZ343369. The average ascarid (length × breadth) were 72.4 ± 3.3 µm (70.5 – 79.9 µm) × 39.9 ± 2.5 µm (37.6 – 42.3 µm). The distinguishing morphological characteristics that have been noticed in ascarid worms were creamy white, cylindrical worm with triradiate lips with wide cephalic alae extending on both the lateral sides and filariform esophagus. In males, spicules were almost equal with the presence of precloacal chitinous-rimmed sucker. The prevalence of A. columbae infection was (63.1%) with a higher incidence in females (79.2%) than males (46.1%). The highest seasonal prevalence was observed in winter (92.5%), followed by summer and spring (87.5% and 55%), respectively while, the lowest prevalence was observed in autumn (17.5%). The intensity of worms in the infected intestine varied from 5 to 120 adult worms. The histopathological examination revealed the presence of chronic diffuse moderate catarrhal enteritis with roundworms in the lumen. Infected birds showed a significant increase in nitric oxide and MDA levels while serum zinc levels were lowered in infected pigeons. Infected pigeons revealed a marked increase in IL1-β and TNFα than apparently healthy ones.

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“…Different parameters of oxidative stress were evaluated in sera. The levels of malondialdehyde (MDA), superoxide dismutase (SOD), and catalase were evaluated using the positive and negative sera with the specific kit analysis [ 17 , 18 ].…”

Section: Methodsmentioning

confidence: 99%

Prevalence, genetic, and biochemical evaluation of immune response of police dogs infected with Babesia vogeli

et al. 2021

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Background and Aim: Babesia species are tick-borne protozoan parasites of apicomplexan type which infect the erythrocytes of dogs it ranges from subclinical to severe cases, depending on different factors such as immune status, age, and presence of other co-infections with the Babesia species. Hence, this study aimed to identify the protozoan parasites infecting police dogs of different breeds, ages, and both sexes in Egypt. Concerning molecular detection of Babesia vogeli using conventional polymerase chain reaction sequencing and phylogenetic analysis, followed by the assessment of immunological and biochemical status of infected dogs. Materials and Methods: The blood of 242 police K9 dogs was collected. The age, breed, sex, and health status with clinical signs of dogs were recorded. Hematological, biochemical, and oxidative stress analyses of the blood were performed together with gene expression analysis using two genes (gamma interferon [IFN-γ] and tumor necrosis factor-alpha [TNF- α]). The identification of the causative agent was performed using molecular analysis of the 18S ribosomal RNA (rRNA). The 18S rRNA region of canine Babesia spp. was successfully amplified, and sequencing data were deposited in GenBank (accession number: MT565474.1), which resembled those of B. vogeli. Results: The results of blood samples screening revealed that of the 242 blood samples, 62 were positive for B. vogeli infection. The infection rate in male dogs was higher than that in female dogs. The police dogs were classified into the following three groups of dogs: (1st group) healthy, (2nd infected with B. vogeli, and mixed infection of B. vogeli and Ehrlichia canis). The oxidative stress biomarkers levels in B. vogeli infected dogs were greater than that of healthy dogs. Likewise, IFN-γ and TNF-α level in B. vogeli infected dogs were elevated in infected dogs. Conclusion: Our findings demonstrated that B. vogeli had completely adverse effects on the health condition of the police dogs that may lead to death in some dogs.

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Effects of chitosan nanoparticles, ivermectin and their combination in the treatment of Gasterophilus intestinalis (Diptera: Gasterophilidae) larvae in donkeys (Equus asinus)

Cited by 23 publications

References 35 publications

Incidence of gastrointestinal parasites in pigeons with an assessment of the nematocidal activity of chitosan nanoparticles against Ascaridia columbae

Incidence of gastrointestinal parasites in pigeons with an assessment of the nematocidal activity of chitosan nanoparticles against Ascaridia columbae

Morphological and molecular characterization of Ascaridia columbae in the domestic pigeon (Columba livia domestica) and the assessment of its immunological responses

Prevalence, genetic, and biochemical evaluation of immune response of police dogs infected with Babesia vogeli

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