Effects of chitosan oligosaccharide and calcium hypochlorite on E. Faecali dentinal biofilm and smear layer removal - SEM analysis

Kaur, Gurveen; Reddy, Tripuravaram Vinay Kumar; Venkatesh, K.; Mahalakshmi, Krishnan

doi:10.4103/ijdr.ijdr_334_20

Cited by 7 publications

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“…The use of irrigation material is expected to be able to clean the root canal from the smear layer and smear plug, increase the adaptation of the obturation material to the dentin wall of the root canal, and penetrate the sealer further into the dentinal tubules. 14 The results of this study indicate that 2% chitosan oligosaccharide solution irrigation has an effect on increasing sealer penetration into dentinal tubules, both in bioceramic sealers and resinbased sealers.…”

Section: Discussionmentioning

confidence: 70%

“…The more acetyl groups released or the greater quantity of free amide active groups (-NH2) present in the depolymerized chitosan molecule, will increase the degree of deacetylation of chitosan. 14 The increase in the degree of deacetylation has an effect on reducing the molecular weight of chitosan which causes a lower viscosity so that chitosan oligosaccharides have a higher solubility in neutral solutions. 15 This leads to the advantages of chitosan oligosaccharides when used as irrigation materials, which can more easily enter the dentinal tubules in the apical third to dissolve the smear layer and smear plug through the chelating effect of chitosan oligosaccharides.…”

Section: Discussionmentioning

confidence: 99%

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<strong>The effect of oligosaccharide chitosan irrigating agent on penetration of two type endodontic sealers into the dentinal tubules: a confocal laser scanning microscopy study</strong>

2022

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Introduction: Irrigation during root canal treatment is one of the important factors that play a role in determining the quality of sealer penetration into the dentinal tubules, especially in the apical third of the tooth root. This is inseparable from the selection of the used irrigation agent. Currently, oligosaccharide chitosan has been developed which is easier to manipulate as an alternative irrigation agent. This study aimed to analyze the effect of the chitosan oligosaccharide irrigating agent on the penetration of two types of endodontic sealers into the dentinal tubules. Methods: This study used 60 mandibular premolars which were randomly divided into 3 groups (n=3). Group 1 was irrigated with 2% chitosan oligosaccharides; Group 2 with a combination of 2.5% NaOCl and 0.2% chitosan nanoparticles; and group 3 was irrigated with a combination of 2.5% NaOCl and 17% EDTA. At the time of obturation, each group was divided into two groups, for sealer treatment using CeraSeal Bioceramic and AH Plus sealer. The sealer’s penetration depth was measured using a confocal laser scanning microscope. Results: The highest mean on the penetration depth of the sealer was seen in the irrigation group using chitosan oligosaccharide solution with CeraSeal bioceramic sealer (2575,151 AU) (p>0.05). Conclusion: There is an effect of 2% oligosaccharide chitosan solution on sealer penetration into dentinal tubules. CeraSeal bioceramic sealer showed better penetration than AH Plus sealer.Keywords: oligosaccharide chitosan; ceraseal bioceramic sealer; AH plus sealer; confocal laser scanning microscope

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

<strong>The effect of oligosaccharide chitosan irrigating agent on penetration of two type endodontic sealers into the dentinal tubules: a confocal laser scanning microscopy study</strong>

2022

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“…Kaur et al (2020) also proved that the combination group of COS with COS solution was the highest in removing the smear layer from other groups and had high antibacterial effectiveness. 17 The microleakage scores in Table 1 show that the restoration at the dentinal margin was higher than that of the enamel in all treatment groups, with an average value of groups 1 and 2. It was because, at the cervical margin, there was little or no enamel in the Class V cavity, so bonding was achieved.…”

Section: Discussionmentioning

confidence: 91%

“…COS is a hydrolysis of chitosan that has a shorter polymer structure, so it has a lower molecular weight and higher solubility than high molecular chitosan. 17,18 COS above, it can be seen that chitosan oligosaccharide has the potential as a cavity cleanser because it has the ideal properties that a cavity cleanser should possess. Research done by Varshneya et al (2017) shows that chitosan has the potential as an effective disinfectant before restoration because it can prevent microleakage and does not interfere with existing adhesive bonds.…”

Section: Discussionmentioning

confidence: 99%

Potential of chitosan oligosaccharide gel as a cavity cleanser against adhesive restoration adhesive on the cavity wall

TARIGAN¹,

ABIDIN²

2023

JDS

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Cavity cleanser is a cavity cleaner with ideal properties such as being biocompatible, antibacterial,removing smear layers, and not interfering with the adhesive bond. This study aims to examine the potential of gelchitosan oligosaccharide (COS) as a cavity cleanser on the adhesion of the restoration to the cavity wall. Thirty-twomandibular premolars with class V restorations are divided into two groups with 2% COS gel and 2% chlorhexidinedigluconate (CHX). Samples were immersed in 2% methylene blue, cut longitudinally, observed under astereomicroscope with 1x magnification, and scored 0-3. The Mann-Whitney test was used to see the potential of gelCOS as a cavity cleanser. The results showed a significant difference between the two groups at the edge of theenamel junction restoration (p=0.038) and the dentin junction restoration (p=0.027). The mean microleakage score ingroup 1 showed better results at the enamel junction (1.19± 1.328) and the dentin junction (2.00±1.211). It shows that2% COS gel has the potential as a cavity cleanser. There was a significant difference between the two groups as acavity cleanser on the adhesion of adhesive restorations to the cavity wall.KEYWORDS: cavity cleanser; chitosan oligosaccharide; chlorhexidine digluconate; microleakage

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“…The evaluation of the smear layer was carried out on apical segments in two studies (Kamble et al, 2017; Penumaka et al, 2019). The others in this evaluation were performed on apical, middle, and coronal segments (Darrag, 2014; İlhan, 2019; Kaur et al, 2020; Madhusudhana et al, 2015; Sari et al, 2018) in Table 1.…”

Section: Resultsmentioning

confidence: 99%

The comparative of chitosan and chitosan nanoparticle versus ethylenediaminetetraacetic acid on the smear layer removal: A systematic review and meta‐analysis of in vitro study

İlhan,

Cakici,

Cakici

2023

Microscopy Res & Technique

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The purpose of this systematic review of meta‐analysis was to compare the effectiveness of removing the smearing layer using EDTA versus Chitosan (Ch) and Chitosan nanoparticles (Ch‐NPs). A search was performed in four electronic databases (Web of Science, PubMed, Scopus, and Cochrane). The included studies were assessed by two reviewers using Joanna Briggs Institute's critical appraisal checklist for the quasi‐experimental studies. Outcomes obtained by scanning electron microscopy (SEM) and conventional methods were presented as standardized mean differences alongside 95% confidence intervals. Seven investigations employed 212 single‐root teeth. In the apical section (p = .317, 95% CI = −0.820 to 0.266, Tau2 = 0.387), middle segment (p = .914, 95% CI = −1.019 to 0.912, Tau2 = 1.027), and coronal segment (p = .277, 95% CI = −1.008 to 0.289, Tau2 = 0.378). This meta‐analysis found no difference between Ch, Ch‐NPs, and EDTA in removing the smear layer in the three segments. This systematic review is designed to show evidence related to the PICO question, in which our outcome is smear layer removal and not the clinical success of such a treatment.Research Highlights The study aimed to compare the effectiveness of chitosan and chitosan nanoparticles with ethylenediaminetetraacetic acid (EDTA) in removing the smear layer, a layer of debris and organic material on the tooth surface, through a systematic review and meta‐analysis. The removal of the smear layer is crucial for successful dental treatments, as it enhances the adhesion of restorative materials and improves the penetration of antimicrobial agents into dentinal tubules. The researchers conducted a systematic review and meta‐analysis, searching various databases of electron microscopy results for relevant in vitro studies comparing the effects of chitosan or chitosan nanoparticles with EDTA on smear layer removal. The results encourage further exploration of chitosan and chitosan nanoparticles for clinical use in dentistry, while considering their specific applications and long‐term effects.

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Effects of chitosan oligosaccharide and calcium hypochlorite on E. Faecali dentinal biofilm and smear layer removal - SEM analysis

Cited by 7 publications

References 0 publications

<strong>The effect of oligosaccharide chitosan irrigating agent on penetration of two type endodontic sealers into the dentinal tubules: a confocal laser scanning microscopy study</strong>

<strong>The effect of oligosaccharide chitosan irrigating agent on penetration of two type endodontic sealers into the dentinal tubules: a confocal laser scanning microscopy study</strong>

Potential of chitosan oligosaccharide gel as a cavity cleanser against adhesive restoration adhesive on the cavity wall

The comparative of chitosan and chitosan nanoparticle versus ethylenediaminetetraacetic acid on the smear layer removal: A systematic review and meta‐analysis of in vitro study

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