Effects of Chlorhexidine Diacetate and Povidone‐lodine on Wound Healing in Dogs

Sanchez, Isis R.; Swaim, Steven F.; Nusbaum, Kenneth E.; Hale, Anne; Henderson, Ronald D.; McGuire, John A.

doi:10.1111/j.1532-950x.1988.tb01019.x

Cited by 95 publications

(43 citation statements)

References 8 publications

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“…Several inactivating reactive substances of the blood and serum may additionally reduce the substantivity of iodine. This might explain that albeit cytotoxic effects in vitro , wound healing in vivo is less affected [13]. In addition, reparative and regenerative periodontal processes are guided by the surrounding tissues, namely epithelium, connective tissue, periodontal ligament and bone which act as a source of growth factors and cells.…”

Section: Discussionmentioning

confidence: 99%

Effect of Short-Time Povidone-Iodine Application on Osteoblast Proliferation and Differentiation

Schmidlin¹,

Imfeld²,

Sahrmann³

et al. 2009

TODENTJ

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Background and Objective:Povidone-iodine [polyvinylpyrrolidone-iodine complex (PVP-I)] is a broad-spectrum antimicrobial agent, frequently used in dentistry. In this study we investigated the short- and longterm effects on osteoblast number, viability, and function after short exposure to PVP-I with and without additional bone-morphogenetic protein-2 (BMP-2). Material and Methods:Confluent osteoblast-like cell line (MC3T3-E1, subclone 24) cultures were exposed to pure PVP-I solution (7.7 mg/ml) and dilutions of 1:10, 1:100 and 1:1000 for 10 seconds and washed with phosphate buffer solution. Cell proliferation and viability was determined by MTT and differentiation status by alkaline phosphatase (ALP) activity 6 days after initial plating. In a separate experiment, long-term cell proliferation, viability and function were assessed 4 weeks after PVP-I treatment by MTT and deposited calcium using an Alizarin-red staining test.Results:PVP-I decreased ALP activity substantially. Stimulation by BMP-2 recovered ALP activity to near control levels at 1:100 and 1:1000 dilutions of PVP-I. The MTT assay showed reduced proliferation of the preosteoblastic cells for all treatments, irrespective whether BMP-2 was used or not. Only at PVP-I dilutions of 1:1000 proliferation rate was back to normal levels (95.6±2.4 %). No adverse long-term effect of PVP-I on mineralization of the extracellular matrix (Alizarinred) for dilutions higher than 1:100 was observed. Interestingly, undiluted and 1:10 diluted PVP-I even showed a significant increase in mineral deposition, especially in the presence of BMP-2.Conclusion:Short-time application of PVP-I in concentrations of 1:10 and higher lead to decreased viability and impaired differentiation. However, surviving cells showed good recovery and mineralization potential.

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Section: Discussionmentioning

confidence: 99%

Effect of Short-Time Povidone-Iodine Application on Osteoblast Proliferation and Differentiation

Schmidlin¹,

Imfeld²,

Sahrmann³

et al. 2009

TODENTJ

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“…In contrast to that, the clinical use of CHX after oral surgical interventions, tooth extractions or implant placement showed significantly less signs of inflammation and improvement of gingival health by reduction of the microbial contamination of the wound [19][20][21]. A further disadvantage is given by the inactivation of CHX by binding on blood, serum proteins and sulcus fluid which was shown in in vitro studies [22,23].…”

Section: Introductionmentioning

confidence: 94%

Antibacterial effect of taurolidine (2%) on established dental plaque biofilm

2011

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Preliminary data have suggested that taurolidine may bear promising disinfectant properties for the therapy of bacterial infections. However, at present, the potential antibacterial effect of taurolidine on the supragingival plaque biofilm is unknown. To evaluate the antibacterial effect of taurolidine on the supragingival plaque biofilm using the vital fluorescence technique and to compare it with the effect of NaCl and chlorhexidine (CHX), 18 subjects had to refrain from all mechanical and chemical hygiene measures for 24 h. A voluminous supragingival plaque sample was taken from the buccal surfaces of the lower molars and wiped on an objective slide. The sample was then divided into three equal parts and mounted with one of the three test or control preparations (a) NaCl, (b) taurolidine 2% and (c) CHX 0.2%. After a reaction time of 2 min, the test solutions were sucked of. Subsequently, the plaque biofilm was stained with fluorescence dye and vitality of the plaque flora was evaluated under the fluorescence microscope (VF%). Plaque samples treated with NaCl showed a mean VF of 82.42 ± 6.04%. Taurolidine affected mean VF with 47.57 ± 16.60% significantly (p < 0.001, paired t test). The positive control CHX showed the lowest mean VF values (34.41 ± 14.79%; p < 0.001 compared to NaCl, p = 0.017 compared to taurolidine). Taurolidine possesses a significant antibacterial effect on the supragingival plaque biofilm which was, however, not as pronounced as that of CHX.

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“…In contrast to polyhexanide, povidone iodide, hydrogen peroxide and chlorhexidine do not lose full antiseptic activity in the presence of blood or other liquids [15,20]. Furthermore, treatment with 0.05 % chlorhexidine has no negative effects on wound healing [21,22]. When used in lavage, chlorhexidine 0.05 % eliminates 99.8 % of contaminating bacteria within one minute in a tissue model [20].…”

Section: Introductionmentioning

confidence: 99%

Human osteoblast damage after antiseptic treatment

Vörös

Dobrindt

Perka

et al. 2013

International Orthopaedics (SICOT)

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Purpose Antiseptics are powerful medical agents used for wound treatment and decontamination and have a high potential for defeating joint infections in septic surgery. Both chlorhexidine and polyhexanide are frequently used in clinical practice and have a broad antimicrobial range, but their effect on human osteoblasts has not been sufficiently studied. Our objective was to investigate the toxic effects of polyhexanide and chlorhexidine on human osteoblasts in vitro to evaluate their clinical applicability in septic surgery. Methods We isolated and cultivated human osteoblasts in vitro and assayed the toxic effects of chlorhexidine 0.1 % and polyhexanide 0.04 %, concentrations commonly applied in clinical practice. Toxicity analysis was performed by visualisation of cell structure, lactate dehydrogenase (LDH) activity and evaluation of vital cells. Toxicity was evaluated by microscopic inspection of cell morphology, trypan blue staining and determination of LDH release. Results Damaged cell structure could be shown by microscopy. Both antiseptics promoted LDH activity after incubation with osteoblasts. The evaluation of vital osteoblasts showed a significant decrease of vital cells. Conclusions Both antiseptics induced significant cell death of osteoblasts at optimum exposure. We therefore recommend cautious use of polyhexanide and chlorhexidine in septic surgery to avoid severe osteoblast toxicity.

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Effects of Chlorhexidine Diacetate and Povidone‐lodine on Wound Healing in Dogs

Cited by 95 publications

References 8 publications

Effect of Short-Time Povidone-Iodine Application on Osteoblast Proliferation and Differentiation

Effect of Short-Time Povidone-Iodine Application on Osteoblast Proliferation and Differentiation

Antibacterial effect of taurolidine (2%) on established dental plaque biofilm

Human osteoblast damage after antiseptic treatment

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