Effects of chlorogenic acid on neutrophil locomotion functions in response to inflammatory stimulus

Hebeda, Cristina Bichels; Bolonheis, Simone Marques; Nakasato, André; Belinati, Karen Daher; Souza, Patrícia da Cruz; Gouvea, Dayana Rubio; Lopes, Norberto Peporine; Farsky, Sandra Helena Poliselli

doi:10.1016/j.jep.2011.02.033

Cited by 37 publications

(23 citation statements)

References 38 publications

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“…Such adipose cells experience endoplasmic reticulum stress and activation of the inflammatory pathway. 13,14 Furthermore, CGA has anti-inflammatory effects, improves insulin sensitivity, and prevents metabolic diseases by affecting the TLR4, 15 NF-κB, 16,17 and JNK/AP-1 pathways 18 as well as immune cell infiltration 19 in the liver, adipose tissue, and muscle. 9 Chlorogenic acid (CGA) is a polyphenolic compound with many isomers and derivatives.…”

Section: Introductionmentioning

confidence: 99%

Effect of 5-caffeoylquinic acid on the NF-κB signaling pathway, peroxisome proliferator-activated receptor gamma 2, and macrophage infiltration in high-fat diet-fed Sprague–Dawley rat adipose tissue

et al. 2015

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Obesity, considered as a consequence of overnutrition, sustains a low-degree inflammatory state and results in insulin-resistance and type 2 diabetes. Here, we investigated the anti-inflammatory effects of 5-caffeoylquinic acid (5-CQA) in high-fat diet-induced obese rats. Serum interleukin (IL)-6, monocyte chemotactic protein 1 (MCP-1), tumor necrosis factor-alpha (TNF-α), total cholesterol (TC), triglyceride (TG), and free fatty acid (FFA) levels were determined. Expression of genes related to TG metabolism, macrophage biomarkers, and inflammation was assessed by real-time PCR. Protein expression of NF-κB, PPARγ2, and phosphorylated IκBα was evaluated by western blotting, and the histology of adipose tissue was examined. Supplementation of the rat diet with 5-CQA reduced obesity development, macrophage infiltration, and steatosis. Additionally, 5-CQA decreased the expression of NF-κB and downstream inflammatory cytokines, but increased the expression of PPARγ2, in a dose-dependent manner. Thus, 5-CQA improved obesity and obesity-related metabolic disturbances via PPARγ2 and the NF-κB signaling pathway.

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Section: Introductionmentioning

confidence: 99%

Effect of 5-caffeoylquinic acid on the NF-κB signaling pathway, peroxisome proliferator-activated receptor gamma 2, and macrophage infiltration in high-fat diet-fed Sprague–Dawley rat adipose tissue

et al. 2015

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“…Thus, it credits the hypothesis that CGA is an important natural product present in L. drymoglossoides . In addition, because of the resemblance between the pharmaceutical properties of CGA (anti-inflammation,[8] antifungal activity,[9] and antioxidant[10]) and the efficacy of L. drymoglossoides , the hypothesis seemed more convincing. Eventually, our initial investigation on the phytochemicals of L. drymoglossoides verified the hypothesis.…”

Section: Introductionmentioning

confidence: 99%

A validated UV-HPLC method for determination of chlorogenic acid in Lepidogrammitis drymoglossoides (Baker) Ching, Polypodiaceae

et al. 2012

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Background:Lepidogrammitis drymoglossoides (Baker) Ching (L. drymoglossoides), a member of the Polypodiaceae family, was used in the treatment of numerous diseases. However, none of the potential ingredients and the quality control methods concerning this plant medicine was pronounced.Objective:To identify chlorogenic acid (CGA) from L. drymoglossoides and develop a high performance liquid chromatography (HPLC) assay of CGA.Materials and Methods:UV, TLC, and HPLC were utilized to identify the phytochemicals of L. drymoglossoides and determine the CGA content, respectively. The HPLC conditions were as following: a Phenomenex Luna C18 (2) (250 × 4.6 mm i.d.; 5 μm particle size; 100 Å pore size) column; the mobile phase of the mixture of acetonitrile and 0.5% aqueous phosphoric acid (11.5:88.5 v/v); the flow rate of 1.0 mL/min and determination wavelength of 327 nm.Results:The proposed HPLC method has been developed and validated. The calibration curve was y = 28328x + 16610 (R2 = 0.9997). The intra-day and inter-day precision and intermediate precision were validated with the RSD less than 5%. The mean recovery rate of the method ranged from 95% to 104%, with the RSD less than 5%. The LOD and LQD values were 0.049 and 0.132 mg/L, respectively. The content of CGA in L. drymoglossoides approximately reached 0.24% (v/v) by the proposed extraction and determination methods.Conclusion:The assay method was simple, convenient, and accurate to the quantification of CGA and can be used for the quality control of the herb.

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“…It is noteworthy that LPS interacts with Toll‐like receptor and stimulates the NFκB and MyD88 pathways, resulting in the synthesis and secretion of inflammatory molecules, including adhesion molecules (Kawai and Akira, ). It is a well‐established protocol in our studies, and for these reasons, it was used in the present manuscript (Hebeda et al , ). In fact, data obtained showed that compound 6 did not induce any alteration on L ‐selectin and β2‐integrin molecule expressions (Figs.…”

Section: Resultsmentioning

confidence: 99%

Beyond Organoleptic Characteristics: The Pharmacological Potential of Flavonoids and their Role in Leukocyte Migration and in L‐Selectin and β2‐Integrin Expression During Inflammation

Suyenaga

Klein‐Júnior

Passos

et al. 2014

Phytotherapy Research

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Flavonoids are compounds responsible for several organoleptic characteristics of plant-derived foods. They are also bioactive compounds with antiinflammatory role. Different mechanisms for this activity have been reported, but their effects on cell migration are not fully understood. In the present study, the role of flavonoids on leukocyte migration in vivo was investigated, using the carrageenan-induced pleurisy model and intravital microscopy in rats. It was found that quercetin (1), rutin (2), flavone (5), apigenin (6) and flavonol (7) reduced cell migration to the pleural cavity and inhibited rolling, adhesion and transmigration. Additionally, flow cytometry assays showed that the in vitro treatment with all compounds (15-60 µM) did not cause cell death and 1 inhibited the cleavage of L-selectin and the β2-integrin expression, whereas 2 and 7 only inhibited the β2-integrin expression. Together, data herein presented clearly show the ability of flavonoids to inhibit in vivo neutrophil influx into inflamed tissue, by acting in different mechanisms of neutrophil migration.

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Effects of chlorogenic acid on neutrophil locomotion functions in response to inflammatory stimulus

Abstract: Based on these findings, we highlight the direct inhibitory actions of CGA on adhesive and locomotion properties of neutrophils, which may contribute to its anti-inflammatory effects and help to explain the use of Lychnophora salicifolia as an anti-inflammatory agent.

Cited by 37 publications

References 38 publications

Effect of 5-caffeoylquinic acid on the NF-κB signaling pathway, peroxisome proliferator-activated receptor gamma 2, and macrophage infiltration in high-fat diet-fed Sprague–Dawley rat adipose tissue

Effect of 5-caffeoylquinic acid on the NF-κB signaling pathway, peroxisome proliferator-activated receptor gamma 2, and macrophage infiltration in high-fat diet-fed Sprague–Dawley rat adipose tissue

A validated UV-HPLC method for determination of chlorogenic acid in Lepidogrammitis drymoglossoides (Baker) Ching, Polypodiaceae

Beyond Organoleptic Characteristics: The Pharmacological Potential of Flavonoids and their Role in Leukocyte Migration and in L‐Selectin and β2‐Integrin Expression During Inflammation

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