Effects of chlorpyrifos on the metabolic profiling of Bacillus megaterium strain RRB

Zhang, Mingxia; Li, Yong; Mu, Qi'e; Feng, Fayun; Yu, Xiangyang; Ge, Jing; Zhang, Yun; Nie, Jinfang

doi:10.1016/j.chemosphere.2022.134189

Cited by 8 publications

(4 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this context, the amino acids glutamate and aspartate could act as substrates to synthesize proteins and other essential substances required by the strain Achromobacter sp. C1 to relieve the toxicity of degradation products of IPR, as observed during the degradation process of chlorpyrifos by Bacillus megaterium strain RRB [55]. Nevertheless, other enzymes like N-ethylammeline chlorohydrolase, N-formylglutamate deformylase, and N-formylglutamate amidohydrolase have not been previously identified in the metabolic pathway of strain C1, and the overexpression in response solely to the presence of IPR could indicate some responsibility in the IPR degradation process.…”

Section: Discussionmentioning

confidence: 99%

“…According to our results, the main negative effects of IPR on strain C1 involve nicotinamide metabolism and benzoate degradation in the tryptophan metabolism, as a consequence of direct inhibition in the nicotinamide pathway but also indirectly due to the effect on FOR in phenylacetate, which is related as an intermediary in both phenylalanine metabolism and the pathway of styrene degradation. Nicotinamide is a compound with a diverse biological function, serving as a precursor of the coenzymes NAD+ and NADP+ related to glycolysis, respiration, and fatty acid synthesis [55]. Primary metabolic pathways, such as the metabolism of carbohydrates and fatty acids for energy production, are needed to stabilize a new homeostasis in the resistant bacteria [56].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolic Profiling and Comparative Proteomic Insight in Respect of Amidases during Iprodione Biodegradation

Donoso-Piñol,

Briceño,

Evaristo

et al. 2023

Microorganisms

View full text Add to dashboard Cite

The fungicide iprodione (IPR) (3-(3,5-dichlorophenyl) N-isopropyl-2,4-dioxoimidazolidine-1-carboxamide) is a highly toxic compound. Although IPR has been restricted, it is still being applied in many places around the world, constituting an environmental risk. The biodegradation of IPR is an attractive option for reducing its residues. In this study, we isolated thirteen IPR-tolerant bacteria from a biopurification system designed to treat pesticides. A study of biodegradation using different strains was comparatively evaluated, and the best degradation rate of IPR was presented by Achromobacter sp. C1 with a half-life (T1/2) of 9 days. Based on a nano-LC-MS/MS analysis for the strains, proteins solely expressed in the IPR treatment were identified by highlighting the strain Achromobacter sp. C1, with 445 proteins primarily involved in the biosynthesis of secondary metabolites and microbial metabolism in diverse environments. Differentially expressed protein amidases were involved in six metabolic pathways. Interestingly, formamidase was inhibited while other cyclases, i.e., amidase and mandelamide hydrolase, were overexpressed, thereby minimizing the effect of IPR on the metabolism of strain C1. The dynamic changes in the protein profiles of bacteria that degrade IPR have been poorly studied; therefore, our results offer new insight into the metabolism of IPR-degrading microorganisms, with special attention paid to amidases.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Metabolic Profiling and Comparative Proteomic Insight in Respect of Amidases during Iprodione Biodegradation

Donoso-Piñol,

Briceño,

Evaristo

et al. 2023

Microorganisms

View full text Add to dashboard Cite

show abstract

“…The metabolic profiles of rice root, stem, and leaf tissues were determined via HPLC-QTOF/MS (LC-20A-TripleTOF 5600+, AB Sciex, Framingham, MA, USA) based on Zhang's detection method [23]. XSelect ® HSS T3 column (2.1 mm × 150 mm, 3.5 µm, Waters, Milford, MA, USA) was used for chromatographic separation.…”

Section: Sample Detectionmentioning

confidence: 99%

Assessing the Effects of Cadmium Stress on the Growth, Physiological Characteristics, and Metabolic Profiling of Rice (Oryza sativa L.) Using HPLC-QTOF/MS

Lan,

He,

Zhang

et al. 2023

Chemosensors

Self Cite

View full text Add to dashboard Cite

Cadmium (Cd) pollution is an important environmental problem, as it is easily absorbed by plants and gradually accumulates in the human body through the food chain. This study aimed to elucidate the changes in the metabolic response of the rice cultivar “TanLiangYou215” under Cd stress. Rice was grown in soil culture at 0 (Control), 2 (Low group), and 10 (High group) mg/kg CdCl2 for 90 days. The ultrastructural, Cd content, antioxidant activity, and metabolic changes to the rice in different tissues were analyzed. Phenotypic characterization and ultrastructure showed that the rice roots and leaves were significantly damaged and plant growth was inhibited in the High group, while plant growth was promoted in the Low group. Overall, Cd showed a regularity of “low promotion and high inhibition”. Physiological indices revealed that rice was significantly affected by Cd stress. Compared to the Control, Cd stress resulted in higher antioxidant enzyme activities, and the Low group suffered less oxidative damage than the High group. Metabolomic studies revealed that Cd stress significantly altered the metabolic profiles of rice plants. Rice responded to Cd stress by upregulating amino acids and regulating related pathways, including alanine, aspartate and glutamate metabolism, and arginine and proline metabolism. The significant expression of flavonoids with antioxidant properties helped rice resist the oxidative damage caused by Cd accumulation in the root tissue; Cd stress significantly downregulated glycerophospholipid metabolism in the stem and leaf tissues, which affected the cellular activities in rice stem and leaf tissues. We investigated the effects of Cd stress on ultrastructure, antioxidant activity, and metabolic changes in different tissues of the rice variety TLY215. Moreover, the different tissues of TLY215 can regulate these metabolic pathways to resist Cd stress, which provides valuable insights into the response of TLY215 to different concentrations of Cd.

show abstract

“…The most commonly employed analytical techniques are liquid chromatography (LC)-tandem mass spectrometry (MS/MS) and nuclear magnetic resonance (NMR). Compared to NMR, LC-MS/MS offers superior resolution of chromatographic peaks, heightened sensitivity, and greater efficiency [20,21]. Untargeted metabolomics, a widely employed approach for qualitative sample analysis, can rapidly identify and classify metabolites based on differences in metabolic pathway maps, and based on LC-MS/MS, can reliably analyze metabolic profiles [22,23].…”

Section: Introductionmentioning

confidence: 99%

Elucidating Softening Mechanism of Honey Peach (Prunus persica L.) Stored at Ambient Temperature Using Untargeted Metabolomics Based on Liquid Chromatography-Mass Spectrometry

Kong,

Luo,

Chen

et al. 2023

Horticulturae

View full text Add to dashboard Cite

Peach fruit softening is the result of a series of complex physiological and biochemical reactions that influence shelf life and consumer acceptance; however, the precise mechanisms underlying softening remain unclear. We conducted a metabolomic study of the flesh and peel of the honey peach (Prunus persica L.) to identify critical metabolites before and after fruit softening. Compared to the pre-softening profiles, 155 peel metabolites and 91 flesh metabolites exhibited significant changes after softening (|log2(FC)| > 1; p < 0.05). These metabolites were mainly associated with carbohydrate metabolism, respiratory chain and energy metabolism (citrate cycle, pantothenate and CoA biosynthesis, nicotinate and nicotinamide metabolism, and pentose and glucuronate interconversions), reactive oxygen species (ROS) metabolism, amino acid metabolism, and pyrimidine metabolism. During peach fruit softening, energy supply, carbohydrate and amino acid metabolism, oxidative damage, and plant hormone metabolism were enhanced, whereas amino acid biosynthesis and cell growth declined. These findings contribute to our understanding of the complex mechanisms of postharvest fruit softening, and may assist breeding programs in improving peach fruit quality during storage.

show abstract

Effects of chlorpyrifos on the metabolic profiling of Bacillus megaterium strain RRB

Cited by 8 publications

References 59 publications

Metabolic Profiling and Comparative Proteomic Insight in Respect of Amidases during Iprodione Biodegradation

Metabolic Profiling and Comparative Proteomic Insight in Respect of Amidases during Iprodione Biodegradation

Assessing the Effects of Cadmium Stress on the Growth, Physiological Characteristics, and Metabolic Profiling of Rice (Oryza sativa L.) Using HPLC-QTOF/MS

Elucidating Softening Mechanism of Honey Peach (Prunus persica L.) Stored at Ambient Temperature Using Untargeted Metabolomics Based on Liquid Chromatography-Mass Spectrometry

Contact Info

Product

Resources

About