Effects of cholecystokinin-octapeptide on the human gallbladder both in vivo and in vitro

Takahashi, Toshio; Yamamura, Takehira; Ishikawa, Yoshihiro; Kantoh, Masaru; Utsunomiya, Joji

doi:10.1007/bf02775940

Cited by 13 publications

(4 citation statements)

References 10 publications

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“…Other gastric, duodenal and small intestinal motor activities, such as number, duration, frequency and amplitude of phase III and cycle period of MMCs, showed no significant differences among the three groups of dogs, as was also the case for the motor response of the gallbladder to exogen‐ ous CCK‐OP. This dose of CCK‐OP (40 ng kg –1 ) acts directly on gallbladder smooth muscle and not through cholinergic nerves 11 –13 . This means that there were no significant differences in contractile forces of the gallbladder smooth muscle among the three groups of dogs.…”

Section: Discussionmentioning

confidence: 99%

Preserved extrinsic neural connection between gallbladder and residual stomach is essential to prevent dysmotility of gallbladder after distal gastrectomy

Tsukamoto

Enjoji

Kanematsu

2000

Neurogastroenterology Motil

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Many reports have been made on gallbladder dysfunction after gastric surgery. We reported that the gallbladder contractions were abolished after antrectomy. Our hypothesis is that preservation of the neural connection along gastric vessels maintains normal gallbladder function during fasting after antrectomy. Six dogs underwent antrectomy with preservation of the extrinsic nerves and six other dogs underwent conventional antrectomy with dissection of the extrinsic nerves and vessels. Laparotomy alone was performed on another six control dogs. Motor activities of the gallbladder and upper gastrointestinal tract were recorded by strain gauges in conscious dogs. Motilin and CCK-OP were used for pharmacological interventions. In conventional antrectomy, the amplitudes of the cyclic motor activity of the gallbladder and the stomach during fasting were significantly reduced, while this activity was maintained in dogs when extrinsic nerves were preserved. The coordination of the motor activity among the gallbladder, stomach and the duodenum was not impaired in the dogs when extrinsic nerves were preserved. The contractions of the gallbladder induced by exogenous motilin were reduced significantly in conventional antrectomy. Therefore, the preserved neural connection along the gastric vessels maintains normal gallbladder function in the fasting state even after distal gastrectomy.

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Section: Discussionmentioning

confidence: 99%

Preserved extrinsic neural connection between gallbladder and residual stomach is essential to prevent dysmotility of gallbladder after distal gastrectomy

Tsukamoto

Enjoji

Kanematsu

2000

Neurogastroenterology Motil

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“…Classically, cholecystokinin has been associated with the gastrointestinal tract, where a family of gastrinand CCK-like polypeptides have been shown to contribute to the regulation of gastrointestinal function and motility (Vizi et al, 1973). The overriding influence of CCK on gut motility appears to be exerted by a direct action on the smooth muscle and also by an interaction with cholinergic mechanisms, such that CCK is able to evoke atropine-sensitive and -insensitive effects (Rakovska et al, 1986;Takahashi et al, 1986;Yamamura et al, 1986). More recently, evidence obtained from bovine gallbladder muscularis has led to the suggestion of a functional excitatory CCK receptor (Schjoldager et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

Cholecystokinin‐octapeptide constricts guinea‐pig and human airways

Stretton

Barnes

1989

British J Pharmacology

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Cholecystokinin‐octapeptide (CCK‐OP, 10−10‐3 × 10−6 m) produced a concentration‐dependent contractile response in guinea‐pig trachea which was enhanced by both the mechanical removal of the epithelium and by indomethacin (10−5 m), with an EC50 of 6.18 ± 0.10 × 10−8 m. Sub‐threshold concentrations of CCK‐OP, which did not alter the resting tone of the smooth muscle, did not alter responses produced to electrical field stimulation (EFS) or to vagal nerve stimulation in an intact tracheal tube preparation. Atropine (2 × 10−6 m) did not alter the concentration‐response curve to CCK‐OP, indicating that CCK‐OP contraction is not mediated by cholinergic mechanisms. The inhibition of neutral endopeptidase (endopeptidase‐24.11).by phosphoramidon (10−5 m) gave a leftward shift in the CCK‐OP concentration‐response curve in tissues with intact epithelium obtained from normal animals, but had no effect in tissues denuded of epithelium or in tissues obtained from animals which had been actively sensitized and challenged with ovalbumin (OA). CCK‐OP‐induced contractile responses were antagonized by the CCK‐receptor antagonists dibutyryl cyclic guanosine monophosphate (pA2 = 4.3) and L‐364,718 (pA2 = 9.6). CCK‐OP induced bronchoconstriction in large, but not small, human airways and was antagonized by the CCK‐receptor antagonist L‐364,718. CCK‐OP had no effect on cholinergic neural responses elicited by EFS in human airways.

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“…Cholecystokinin (CCK) was first detected in the mammalian small intestine, stimulating the release of pancreatic enzymes and contraction of the gallbladder . The five C‐terminal amino acid residues of CCK are identical to those of gastrin, and both peptides are considered to have evolved from the same ancestral molecule .…”

Section: Introductionmentioning

confidence: 99%

Intracerebroventricular administration of sulphated cholecystokinin octapeptide induces anxiety‐like behaviour in goldfish

Sachuriga

Iinuma

Shibata

et al. 2019

J Neuroendocrinology

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Sulphated cholecystokinin octapeptide (CCK‐8s) is involved in feeding regulation as an anorexigenic neuropeptide in vertebrates. In rodents, i.c.v. administration of CCK‐8s has been shown to affect not only feeding behaviour, but also psychomotor activity. However, there is still no information available concerning the psychophysiological effects of CCK‐8s in goldfish. Therefore, in the present study, we examined the effect of synthetic goldfish (gf) CCK‐8s on psychomotor activity in this species. Intracerebroventricular administration of gfCCK‐8s at 0.1, 0.5 and 2.5 pmol g‐1 body weight (BW) did not affect swimming distance (locomotor activity). Because goldfish prefer the lower to the upper area of a tank, we used this as a preference test (upper/lower test) to assess anxiety‐like behaviour. Intracerebroventricular administration of gfCCK‐8s at 2.5 pmol g‐1 BW shortened the time spent in the upper area. The action of gfCCK‐8s mimicked that of FG‐7142 (the central‐type benzodiazepine receptor inverse agonist, an anxiogenic agent) at 5 and 10 pmol g‐1 BW. The anxiogenic‐like effect of gfCCK‐8s was abolished by treatment with the CCK receptor antagonist proglumide at 50 pmol g‐1 BW. We also investigated the localisation of CCK/gastrin‐like immunoreactivity in the goldfish brain. CCK/gastrin‐like immunoreactivity was observed in the anxiety‐related regions (the nucleus habenularis and the interpeduncular nucleus). These data indicate that gfCCK‐8s potently affects psychomotor activity in goldfish, and exerts an anxiogenic‐like effect via the CCK receptor‐signalling pathway.

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Effects of cholecystokinin-octapeptide on the human gallbladder both in vivo and in vitro

Cited by 13 publications

References 10 publications

Preserved extrinsic neural connection between gallbladder and residual stomach is essential to prevent dysmotility of gallbladder after distal gastrectomy

Preserved extrinsic neural connection between gallbladder and residual stomach is essential to prevent dysmotility of gallbladder after distal gastrectomy

Cholecystokinin‐octapeptide constricts guinea‐pig and human airways

Intracerebroventricular administration of sulphated cholecystokinin octapeptide induces anxiety‐like behaviour in goldfish

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