Effects of chondroitin sulfate and sodium hyaluronate on chondrocytes and extracellular matrix of articular cartilage in dogs with degenerative joint disease

Gonçalves, Guilherme Vasques; Melo, Eliane Gonçalves de; Gomes, M.G.; Nunes, V.A.; Rezende, C.M.F.

doi:10.1590/s0102-09352008000100014

Cited by 20 publications

(17 citation statements)

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“…As regards the control joints, the histological findings reflected the degenerative nature of this model as presence of surface cartilage irregularities and fibrillation, edema, hypocellularity alternating with hypercellular & proliferative areas and decrease of the concentration of proteoglycans characterized by the reduction of staining intensity. Such changes are observed in the DJD and were also described by [40,41]. …”

Section: Discussionsupporting

confidence: 70%

Homing and reparative effect of intra-articular injection of autologus mesenchymal stem cells in osteoarthritic animal model

Mokbel

Tookhy

Shamaa

et al. 2011

BMC Musculoskelet Disord

160

178

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BackgroundThis work aimed to study the homing evidence and the reparative effect of mesenchymal stem cells (MSCs) in the healing process of induced osteoarthritis in experimental animal model (donkeys).MethodsTwenty-seven donkeys were equally divided into 3 groups based on the observation period after induction of arthritis (3, 6 and 9 weeks) to achieve different degrees of osteoarthritis. Each group was subdivided into three subgroups of three animals each based on the follow-up period (1, 2 and 6 months) after treatment. The induction was done through intra-articular (IA) injection of 2 ml of Amphotericin-B in both carpal joints. MSCs were harvested in a separate procedure, labeled with green fluorescent protein (GFP) using monster GFP vector and suspended in hyaluronic acid for IA injection. Treatment approaches consisted of cell-treatment using MSCs suspended in 3 ml of hyaluronic acid (HA) for the right carpal joint; and using the same amount of (HA) but without MSCs for the left contralateral carpal joint to serve as a control. Animals were assessed clinically and radiologically before and after treatment. Synovial fluid was also evaluated. Histopathologically; articular cartilage structural changes, reduction of articular cartilage matrix staining, osteophyte formation, and subchondral bone plate thickening were graded. Data was summarized using median and percentile for scores of histopathologic grading. Comparison between groups was done using non-parametric Mann Whitney test.ResultsThe reparative effect of MSCs was significant both clinically and radiologically in all treated groups (P < 0.05) compared to the control groups. Fluorescence microscopy of sections of the cell-treated joints of all animals indicated that the GFP-transduced injected cells have participated effectively in the reparative process of the damaged articular surface and have integrated within the existing articular cartilage. The cells were associated with the surface of the cartilage and, were also detected in the interior.ConclusionsHoming was confirmed by the incorporation of injected GFP-labeled MSCs within the repaired newly formed cartilage. Significant recovery proves that the use of IA injection of autologous MSCs is a viable and a practical option for treating different degrees of osteoarthritis.

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Section: Discussionsupporting

confidence: 70%

Homing and reparative effect of intra-articular injection of autologus mesenchymal stem cells in osteoarthritic animal model

Mokbel

Tookhy

Shamaa

et al. 2011

BMC Musculoskelet Disord

160

178

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“…Although studies have confirmed that parenteral administration of chondroitin sulfate stimulates chondrocytes metabolism and satisfactorily reduces the progression of articular cartilage degeneration in DAD models induced by chronic instability in dog knee (Gonçalves et al, 2008;Melo et al, 2008), the results of the present study clarify that the chondroitin sulfate in association with glucosamine, as a nutraceutical, did not promote the same stimulus in the repair of sharply induced osteochondral defects.…”

Section: Discussioncontrasting

confidence: 69%

Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings

Eleotério

Pontes²,

Machado³

et al. 2015

Arq. Bras. Med. Vet. Zootec.

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Chondroitin and glucosamine sulfate nutraceuticals are commonly used in the management of degenerative articular disease in veterinary routine. However, there are controversies on the contribution of these substances to articular cartilage. The purpose of this study was to evaluate the efficiency of a chondroitin and glucosamine sulfate-based veterinary nutraceutical on the repair of an induced osteochondral defect in a dog femoral condyle, by macroscopic, histological and histomorphometric analyses. The nutraceutical was orally administered the day following injury induction, every 24 hours (treated group, TG, n=24), compared with animals that did not receive the product (control group, CG, n=24). Six animals per group were anaesthetized for sample collection at 15, 30, 60 and 90 days after surgery. At 15 days, defects were macroscopically filled with red-pinkish tissue. After 30 days, whitish color tissue was observed, both in TG and CG animals, with firmer consistency to touch at 60 and 90 postoperative days. Histological analysis demonstrated that, in both groups, there was initial blood clot formation, which was subsequently substituted by a fibrin net, with capillary proliferation from the adjacent bone marrow and infiltration of mesenchymal cells in clot periphery. As cellular differentiation developed, repair tissue presented a fibrocartilage aspect most of the time, and new subchondral bone formation occurred in the deepest area corresponding to the defect. Histomorphometry suggested that the nutraceutical did not favor the articular cartilage repair process. It was concluded that nutraceutical did not significantly influence chondrocytes proliferation or hyaline architecture restoration.Keywords: degenerative articular disease, osteoarthritis, arthrosis, chondroprotective, cartilage RESUMO Os nutracêuticos compostos de sulfato de condroitina e glucosamina são comumente utilizados no manejo da doença articular degenerativa na rotina veterinária. Entretanto, existem controvérsias sobre a contribuição dessas substâncias à cartilagem articular. O objetivo deste trabalho foi avaliar a eficácia de um nutracêutico veterinário à base de sulfato de condroitina e glucosamina na reparação de defeitos osteocondrais induzidos no côndilo femoral de cães, através de análises macroscópica, histológica e histomorfométrica. O nutracêutico foi administrado no dia seguinte à indução da lesão, pela via oral, a cada 24 horas (grupo tratado -GT, 24 animais), sendo comparado a animais que não receberam o produto (grupo controle -GC, de igual número de animais

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“…The model used CCLT to induce morphological changes allowed the evaluation of this common disease, as described by Biasi et al (2005), Gonçalves et al (2008) and Melo et al (2008). The development and progression of OA occurred due to joint instability, which changed the distribution of weight on the joint (Glasson et al 2007, Herzog & Longino 2007.…”

Section: Discussionmentioning

confidence: 99%

Experimental osteoarthritis in rabbits: lesion progression

et al. 2013

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ABSTRACT.-Campos W.N.S., Souza M.A., Ruiz T., Peres T.P., Néspoli P.B., Marques A.T.C., Colodel E.M. & Souza R.L. 2013. Experimental osteoarthritis in rabbits: lesion progression. Pesquisa Veterinária Brasileira 33(3):279-285. Programa de Pós-Graduação em Ciên-cias Veterinárias, Faculdade de Agronomia, Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso, Av. Fernando Corrêa da Costa 2367, Bairro Boa Esperança, Cuiabá, MT 78068-900, Brazil. E-mail: wilmavet@gmail.comThe aim of this study was to evaluate the progression of lesions in different stages of osteoarthritis (OA) experimental by radiography (RX), computed tomography (CT), macroscopic and histopathology, linking these different diagnostic methods, helped to provide information that helps the best time for the therapeutic approach. Four experimental periods were delineated at 3, 6, 9 and 12 weeks after induction of OA, known as PI, PII, PIII and PIV, respectively, each with six animals. We evaluated the five compartments of the femorotibial joint: medial femoral condyle (MFC), lateral femoral condyle (LFC), medial tibial plateau (MTP), lateral tibial plateau (LTP) and femoral trochlea (FT). Therefore we established an index by compartment (IC) and by adding such an index was estimated joint femorotibial (IFT). It was observed that the CFM was the compartment with the highest IC also differed significantly (p<0.05) from other compartments. Compartments showed no significant difference (p>0.05) between the PI and PII, however contrary fact occurred between the PII and PIII (p<0.05), PIII and PIV (p<0.01) and between PI and PIV (p<0.001). Similarly the IFT, showed a significant difference in the animals of PIV compared to PI (p<0.001), PII (p<0.001) and PIII (p<0.01), and there was no statistical difference (p> 0.05) between the PI and PII. In the variation of the average interval between periods, there was a higher value between the PIII PIV and for the other intervals of time periods (PI, PII, and PIII-PII). However, these intervals showed no statistically significant difference (p>0.05). Through the RX, CT, macroscopic and histopathological findings, we found similar patterns among individuals within the same period demonstrating a gradual progression of the disease. These results show that between 3 and 6 weeks progression of the lesion is slower and probably also can be reversed in comparison to other ranges where proved further progression between 9 and 12 weeks after induction of trauma OA. These results may provide a better therapeutic approach aimed at reversing the lesions in early stages of OA. We conclude that the interconnection of the four diagnostic methods individually classified into scores, which were unified in both indices in the evaluation by the femorotibial joint compartment and may represent a diagnostic condition closer to the true condition of the injury and its progression. . Através do RX, TC, macroscopia e histopatológico, verificamos padrões similares entre os indivíduos dentro do mesmo período demonstrando assi...

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Effects of chondroitin sulfate and sodium hyaluronate on chondrocytes and extracellular matrix of articular cartilage in dogs with degenerative joint disease

Cited by 20 publications

References 18 publications

Homing and reparative effect of intra-articular injection of autologus mesenchymal stem cells in osteoarthritic animal model

Homing and reparative effect of intra-articular injection of autologus mesenchymal stem cells in osteoarthritic animal model

Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings

Experimental osteoarthritis in rabbits: lesion progression

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