Effects of chronic dietary selenomethionine exposure on repeat swimming performance, aerobic metabolism and methionine catabolism in adult zebrafish (Danio rerio)

Comparison of the acute effects of benzo-a-pyrene on adult zebrafish (Danio rerio) cardiorespiratory function following intraperitoneal injection versus aqueous exposure

“…In the present study, BaP-injected and aqueously-exposed fish had elevated MO 2 and COT compared to controls which is in agreement with several studies where both of these parameters increased following exposure to toxicants, including PAHs (Shingles et al, 2001;Davoodi and Claireaux, 2007;McKenzie et al, 2007;Thomas et al, 2013). SMR was also increased with both exposure routes compared to controls, indicating that basal metabolism or metabolic loading of BaP-exposed fish was elevated in response to toxicant exposure Santos, 2001, 2002;Tintos et al, 2008).…”

“…Standard metabolic rate (SMR) was calculated by extrapolating consumption of oxygen back to a water velocity of zero using a plot of swim speed (m/s) versus MO 2 (mg O 2 /g/h). This was done using a nonlinear, curve fitting regression analysis (Thomas et al, 2013) with GraphPad Prism software (La Jolla, CA, USA) and using all measurements from completed swim velocity segments for a given fish (4-5 data points per fish). Active metabolic rate was determined for individual fish using the MO 2 values at the maximum swimming velocity in the U crit test.…”

“…Factorial aerobic scope (F-AS) was calculated as AMR/SMR (Killen et al, 2007;Gerger et al, 2014). Cost of transport (J/g/m) was calculated by multiplying MO 2 (mg O 2 /g/s) by an oxycaloric value of 14.1 J/mg O 2 and then dividing by the corresponding swimming speed (m/s) (Thomas et al, 2013).…”

Cloning and characterization of SREBP-1 and PPAR-α in Japanese seabass Lateolabrax japonicus, and their gene expressions in response to different dietary fatty acid profiles

“…No evidence of alternatively spliced mRNAs of SREBP-1 was found in Japanese seabass, although this cannot be excluded since SREBP-1 cDNA was only synthesized from liver RNA in this study. Similar findings were also observed in other teleost, such as Atlantic salmon (Minghetti et al, 2011) and zebrafish (Thomas et al, 2013). The alignments analysis of the deduced amino acid sequences of SREBP-1 from Japanese seabass, other fish, and humans suggested that the SREBP-1 sequences in Japanese seabass, as well as those from other fish, were more similar to human SREBP-1a than to human SREBP-1c, indicating that fish SREBP-1 probably had functions similar to human SREBP-1a.…”

Histopatologia do fígado de tilápias-do-nilo suplementadas com selenometionina

“…No tratamento com 1,0 mg kg -1 , ocorreram hipertrofia dos hepatócitos e presença de núcleos picnóticos deslocados para a periferia da célula (Figura 1 D), enquanto, no tratamento com 1,5 mg kg -1 , havia focos de necrose (Figura 1 E), congestão e presença de grânulos de glicogênio (PAS positivo), quando comparado aos tratamentos anteriores (Figura 1 F). A toxicidade do selênio em doses acima de 1,0 mg kg -1 já havia sido relatada em outras espécies de peixes, como esturjões (Tashjian et al, 2006;Del Riu et al, 2014) e paulistinha (Danio rerio) (Thomas et al, 2013), mas foi constatada pela primeira em tilápias-do-nilo.…”