Effects of Chronic Dim-light-at-night Exposure on Sleep in Young and Aged Mice

Panagiotou, Maria; Deboer, Tom

doi:10.1016/j.neuroscience.2019.11.033

Cited by 19 publications

(14 citation statements)

References 24 publications

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“…Sleep architecture under DLAN exposure was characterized by a gradual delay of vigilance state rhythms in the course of prolonged exposure. Following three months of DLAN exposure, SWA and its 24-h rhythm was considerably attenuated, and EEG spectral alterations emerged in all vigilance states (data partly published previously in [15] Panagiotou and Deboer 2020). Since EEG is thought to be a window on the brain, any divergence there is likely to show underlying neuronal network hindrance [21,22].…”

Section: Introductionsupporting

confidence: 68%

“…A study in mice showed an absence of effects of DLAN (8-10 weeks DLAN, 5 lux) on timing as well as quality of sleep in mice [14]. However, recently we showed that prolonged exposure to DLAN (3 months, 5 lux) changes sleep and EEG characteristics in mice [15]. Hence, more data are needed before a consensus can be reached on the effect of DLAN on sleep.…”

Section: Introductionmentioning

confidence: 93%

“…A group of mice (n = 5) was exposed to DLAN for one month in total. A second group (n = 9) was exposed to DLAN for continuous three months [15] (Panagiotou and Deboer, 2020). The age of all mice at the time of the recordings was six months.…”

Section: Animalsmentioning

confidence: 99%

“…In the present study, as a follow up of our previous chronic DLAN exposure study [15], we investigated the effect of exposure to different DLAN duration periods, i.e., one day, one week, and one month, on rest-activity behavior, sleep architecture, and sleep EEG parameters in C57BL/6J mice. Sleep architecture under DLAN exposure was characterized by a gradual delay of vigilance state rhythms in the course of prolonged exposure.…”

Section: Introductionmentioning

confidence: 99%

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Sleep Network Deterioration as a Function of Dim-Light-At-Night Exposure Duration in a Mouse Model

2020

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Artificial light, despite its widespread and valuable use, has been associated with deterioration of health and well-being, including altered circadian timing and sleep disturbances, particularly in nocturnal exposure. Recent findings from our lab reveal significant sleep and sleep electroencephalogram (EEG) changes owing to three months exposure to dim-light-at-night (DLAN). Aiming to further explore the detrimental effects of DLAN exposure, in the present study, we continuously recorded sleep EEG and the electromyogram for baseline 24-h and following 6-h sleep deprivation in a varied DLAN duration scheme. C57BL/6J mice were exposed to a 12:12 h light:DLAN cycle (75lux:5lux) vs. a 12:12 h light:dark cycle (75lux:0lux) for one day, one week, and one month. Our results show that sleep was already affected by a mere day of DLAN exposure with additional complications emerging with increasing DLAN exposure duration, such as the gradual delay of the daily 24-h vigilance state rhythms. We conducted detrended fluctuation analysis (DFA) on the locomotor activity data following 1-month and 3-month DLAN exposure, and a significantly less healthy rest-activity pattern, based on the decreased alpha values, was found in both conditions compared to the control light-dark. Taking into account the behavioral, sleep and the sleep EEG parameters, our data suggest that DLAN exposure, even in the shortest duration, induces deleterious effects; nevertheless, potential compensatory mechanisms render the organism partly adjustable and able to cope. We think that, for this reason, our data do not always depict linear divergence among groups, as compared with control conditions. Chronic DLAN exposure impacts the sleep regulatory system, but also brain integrity, diminishing its adaptability and reactivity, especially apparent in the sleep EEG alterations and particular low alpha values following DFA.

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Section: Introductionsupporting

confidence: 68%

Section: Introductionmentioning

confidence: 93%

Section: Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sleep Network Deterioration as a Function of Dim-Light-At-Night Exposure Duration in a Mouse Model

2020

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“…However, the studies do not provide a detailed analysis of locomotor activity or the activity of single neurons in SCN; thus, further research is needed in this area. Long-term exposure (3 months) of more than 6 months old mice induced the attenuation of locomotor activity's rhythm [97]. Higher light intensity (20 lx) increased daytime activity and phase-advanced the onset and offset of the locomotor activity [98].…”

Section: Locomotor Activity Under Dim Light At Nightmentioning

confidence: 99%

Differential Effects of Constant Light and Dim Light at Night on the Circadian Control of Metabolism and Behavior

Rumanova

Okuliarova

Zeman

2020

IJMS

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The disruption of circadian rhythms by environmental conditions can induce alterations in body homeostasis, from behavior to metabolism. The light:dark cycle is the most reliable environmental agent, which entrains circadian rhythms, although its credibility has decreased because of the extensive use of artificial light at night. Light pollution can compromise performance and health, but underlying mechanisms are not fully understood. The present review assesses the consequences induced by constant light (LL) in comparison with dim light at night (dLAN) on the circadian control of metabolism and behavior in rodents, since such an approach can identify the key mechanisms of chronodisruption. Data suggest that the effects of LL are more pronounced compared to dLAN and are directly related to the light level and duration of exposure. Dim LAN reduces nocturnal melatonin levels, similarly to LL, but the consequences on the rhythms of corticosterone and behavioral traits are not uniform and an improved quantification of the disrupted rhythms is needed. Metabolism is under strong circadian control and its disruption can lead to various pathologies. Moreover, metabolism is not only an output, but some metabolites and peripheral signal molecules can feedback on the circadian clockwork and either stabilize or amplify its desynchronization.

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Doublecortin‐like expressing astrocytes of the suprachiasmatic nucleus are implicated in the biosynthesis of vasopressin and influences circadian rhythms

Coomans

Saaltink

Deboer

et al. 2021

Glia

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We have recently identified a novel plasticity protein, doublecortin-like (DCL), that is specifically expressed in the shell of the mouse suprachiasmatic nucleus (SCN). DCL is implicated in neuroplastic events, such as neurogenesis, that require structural rearrangements of the microtubule cytoskeleton, enabling dynamic movements of cell bodies and dendrites. We have inspected DCL expression in the SCN by confocal microscopy and found that DCL is expressed in GABA transporter-3 (GAT3)-positive astrocytes that envelope arginine vasopressin (AVP)-expressing cells. To investigate the role of these DCL-positive astrocytes in circadian rhythmicity, we have used transgenic mice expressing doxycycline-induced short-hairpin (sh) RNA's targeting DCL mRNA (DCL knockdown mice). Compared with littermate wild type (WT) controls, DCL-knockdown mice exhibit significant shorter circadian rest-activity periods in constant darkness and adjusted significantly faster to a jet-lag protocol. As DCL-positive astrocytes are closely associated with AVP-positive cells, we analyzed AVP expression in DCL-knockdown mice and in their WT littermates by 3D reconstructions and transmission electron microscopy (TEM). We found significantly higher numbers of AVP-positive cells with increased volume and more intensity in DCL-knockdown mice. We found alterations in the numbers of dense core vesiclecontaining neurons at ZT8 and ZT20 suggesting that the peak and trough of neuropeptide biosynthesis is dampened in DCL-knockdown mice compared to WT littermates. Together, our data suggest an important role for the astrocytic plasticity in the regulation of circadian rhythms and point to the existence of a specific DCL + astrocyte-AVP + neuronal network located in the dorsal SCN implicated in AVP biosynthesis.

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Effects of Chronic Dim-light-at-night Exposure on Sleep in Young and Aged Mice

Cited by 19 publications

References 24 publications

Sleep Network Deterioration as a Function of Dim-Light-At-Night Exposure Duration in a Mouse Model

Sleep Network Deterioration as a Function of Dim-Light-At-Night Exposure Duration in a Mouse Model

Differential Effects of Constant Light and Dim Light at Night on the Circadian Control of Metabolism and Behavior

Doublecortin‐like expressing astrocytes of the suprachiasmatic nucleus are implicated in the biosynthesis of vasopressin and influences circadian rhythms

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