Effects of Chronic Ethanol Consumption and Ovariectomy on the Spontaneous Alveolar Bone Loss in Rats

Abstract

Postmenopausal estrogen deficiency and ethanol (EtOH) abuse are known risk factors for different diseases including bone tissues. However, little is known about the synergic effects of EtOH abuse and estrogen deficiency on alveolar bone loss in women. The present study evaluated the effects of EtOH chronic exposure and ovariectomy on the alveolar bone loss in female rats. For this, 40 female Wistar rats were randomly divided into 4 groups: control, EtOH exposure, ovariectomy (OVX), and OVX plus EtOH exposure. Initially, half of the animals were ovariectomized at 75 days of age. After that, the groups received distilled water or EtOH 6.5 g/kg/day (20% w/v) for 55 days via gavage. Thereafter, animals were sacrificed and the mandibles were collected, dissected, and separated into hemimandibles. Alveolar bone loss was evaluated by measuring the distance between the cementoenamel junction and the alveolar bone crest through a stereomicroscope in 3 different anatomical regions of the tissue. One-way ANOVA and post hoc Tukey were used to compare groups ( p < 0.05 ). The results showed that the ovariectomy and EtOH exposure per se were able to induce alveolar bone loss, and their association did intensify significantly the effect. Therefore, OVX associated with heavy EtOH exposure increase the spontaneous alveolar bone loss in rats.

Prophylactic efficiency of the administration of vitamin, mineral and sorbent complexes on bone tissue in female rats against the background of chronic alcohol consumption

“…The influence of alcohol on the state of the alveolar bone is often studied in the presence of concomitant factors such as periodontitis or ovariectomy. Chronic alcoholism in combination with periodontitis or ovariectomy led to alveolar bone loss and decreased bone density (de Almeida et al, 2020;Frazão et al, 2020;Nascimento et al, 2020). The research results showed that ovariectomy and ethanol exposure alone can induce alveolar bone loss, and their combina-tion significantly enhances this effect (Nascimento et al, 2020).…”

“…Chronic alcoholism in combination with periodontitis or ovariectomy led to alveolar bone loss and decreased bone density (de Almeida et al, 2020;Frazão et al, 2020;Nascimento et al, 2020). The research results showed that ovariectomy and ethanol exposure alone can induce alveolar bone loss, and their combina-tion significantly enhances this effect (Nascimento et al, 2020). In the study of the effect of ethanol on bone tissue, although it is noted that the effect of ethanol itself does not lead to an increase in the loss of alveolar bone tissue, the quality of bone tissue changes in all evaluated parameters (trabecular thickness, trabecular number parameter, percent bone density) (Frazão et al, 2020).…”

Imbalance of Antioxidant-Prooxidant System in Rat's Jaw Bone Tissue Under Long-Term Introduction of Ethanol

“…Встановлені нами факти узгоджуються з даними інших дослідників щодо негативного впливу споживання алкоголю на ступінь атрофії альвеолярного відростка [5,15], які показали високий ступень втрати альвеолярного відростка та стимуляцію розвитку пародонтиту після тривалого вживання алкоголю [15].…”