Effects of chronic food restriction and treatments with leptin or ghrelin on different reproductive parameters of male rats

Sirotkin, Alexander V.; Chrenková, M.; Nitrayová, S.; Patráš, P.; Darłak, Krzysztof; Valenzuela, Francisco; Pinilla, Leonor; Tena‐Sempere, Manuel

doi:10.1016/j.peptides.2008.03.011

Cited by 42 publications

(27 citation statements)

References 40 publications

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“…In addition, daily injection of ghrelin during puberty similarly decreased LH levels [39]. In the other study, repeated ghrelin injections in food-restricted males dramatically decreased plasma LH and T concentrations and reduced testis weight [40]. Duran et al [41] demonstrated decreased serum Ghrelin levels in idiopathic hypogonadotropic hypogonadism (IHH) patients who also had lower serum LH and testoste- [42] demonstrated that when the serum testosterone levels were increased, serum ghrelin level was increased significantly.…”

Section: Discussionmentioning

confidence: 93%

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The effects of streptozotocin-induced diabetes on ghrelin expression in rat testis: biochemical and immunohistochemical study

Sönmez

Karabulut

Kilic

et al. 2015

Folia Histochem Cytobiol.

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Introduction. Ghrelin is a hormone which has effects on the secretion of growth hormone, gastrointestinal system, cardiovascular system, cell proliferation and reproductive system. The present study we focused on the relation between ghrelin and GHS-R1a gene expression and the regulation of their expression in the testes of diabetic rats. Material and methods. 40 male Wistar albino rats were divided into four groups: control, and sampled 4, 8 and 12 weeks after induction of diabetes by streptozotocin (STZ) intraperitoneal injection (40 mg/kg). The rats were decapitated under ketamine anesthesia and their testes were removed. Blood was obtained from heart and serum follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone levels were measured by ELISA. Tissue ghrelin and GHS-R mRNA levels were determined by qRT-PCR, while ghrelin protein expression was studied by immunohistochemistry. Histopathological damage scores were also assessed. Results. Eight weeks after diabetes induction serum FSH level was increased, whereas LH and testosterone concentrations decreased. The ghrelin and GHS-R1a gene expression and ghrelin immunohistochemistry score first tended to increase after first four weeks of diabetes, and then tended to decrease. Ghrelin-immunopositive cells were detected in Leydig cells in all groups of rats, however, not in the germinal epithelium. Congestion of vessels and hemorrhage, formation of the vacuoles in spermatogonia and spermatocytes, desquamation of spermatids in the lumen and disorganization of seminiferous tubule germinal epithelium were observed in testis of all the diabetic rats. In addition, mean testicular biopsy score and mean seminiferous tubule diameter were getting lower in diabetic animals. Conclusion. Our results suggest that diabetes affects ghrelin expression in rat testis.

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Section: Discussionmentioning

confidence: 93%

“…Several studies have pointed out the HPG axis as the target of ghrelin function [22,[39][40][41]. However, there are conflicting data on ghrelin levels and gonadotropins levels.…”

Section: Discussionmentioning

confidence: 99%

The effects of streptozotocin-induced diabetes on ghrelin expression in rat testis: biochemical and immunohistochemical study

Sönmez

Karabulut

Kilic

et al. 2015

Folia Histochem Cytobiol.

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“…In fact, Lep and/or Ob-R were detected in rodent testes (Caprio et al, 2003;El-Hefnawy et al, 2000;Herrid et al, 2008), in human testes and seminal plasma (Glander et al, 2002;Soyupek et al, 2005;Ishikawa et al, 2007), in human sperm (Aquila et al, 2005), in boar sperm (De Ambrogi et al, 2007), and in pig sperm (Aquila et al, 2008). Furthermore, a recent paper reported the effects of leptin treatment, alone or in combination with dietary energy restriction, on different reproductive parameters of male rats (Sirotkin et al, 2008).…”

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confidence: 98%

Leptin and Its Receptor Are Expressed in the Testis and in the Epididymis of Young and Adult Pigs

Rago

Aquila

Guido

et al. 2009

The Anatomical Record

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Recent studies indicated that leptin, a 16 kDa hormone, is a regulatory signal in human and rodent male reproduction. This work was designed to investigate the expression of leptin and its receptor in testes and epididymides from immature and mature pigs. Immunolocalization revealed that leptin and its receptor were confined only in the interstitial compartment of immature testes, whereas both proteins were detected in Leydig cells and within seminiferous tubules of mature gonads. The immunostaining of epididymal tissues showed that leptin was absent in the epithelial cells of immature pigs but it was present in all the three regions of mature epididymides, although with a minor signal in the cauda. Conversely, leptin receptor was observed in all the epithelial cells of both immature and mature epididymides. Western blot analysis of tissue extracts detected a 16 kDa band for leptin and five/six isoforms, ranging from 120 to 40 kDa, for leptin receptor. In conclusion, this work has identified, for the first time, leptin and leptin receptor in the testis and in the epididymis of the pig showing a differential cell-type expression pattern of the two proteins in young and adult animals. Therefore, our findings suggest a possible involvement of leptin in endocrine or autocrine/ paracrine control of porcine male reproductive structures. Anat Rec, 292:736-745, 2009. V V C 2009 Wiley-Liss, Inc.

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“…Chronic administration of ghrelin consistently decreased luteinizing hormone (LH) secretion in pubertal and adult male rats (Martini et al 2006). Similarly, a 20-day ghrelin treatment of young adult male rats reduced plasma LH and testosterone, but only in animals exposed to food restriction (Sirotkin et al 2008). Central application of ghrelin significantly decreased serum levels of LH in intact and orchidectomized peripubertal rats, while in vitro ghrelin stimulated the secretion of both gonadotropins (Fernandez-Fernandez et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Ghrelin and its receptor GHS R1a are expressed in rat and human gonads (Tena-Sempere et al 2002;Gaytan et al 2004). Finally, ghrelin is shown to affect testis size and structure (Sirotkin et al 2008;Kheradmand et al 2009) and to modulate testosterone secretion, androgen receptor expression, Leydig cell proliferation and seminiferous tubule gene expression Kheradmand et al 2009;Wang et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Histomorphometric features of ventral prostate in different aged rats after central ghrelin treatment

Ba¹,

Nešić²,

Dm³

et al. 2012

gpb

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Abstract. Ghrelin, the endogenous ligand of growth hormone secretagogue receptor type 1a (GHS-R1a), has emerged as pleiotropic modulator of diverse biological functions, including energy homeostasis and recently, reproduction. The influence of intracerebroventricularly (ICV) administered ghrelin (1 μg/day/rat for 5 days) to rats of different ages, i.e., peripubertal (38 days), adult (60 days) and middle-aged (180 days) on the ventral prostate size and morphology, serum testosterone levels and testis weight was examined. Ghrelin treatment significantly increased (p < 0.05) absolute ventral prostate weight in peripubertal and middle-aged rats, by 27% and 37% respectively, due to enhancement of epithelial and/or luminal compartment of the gland. In adult rats, both absolute and relative volumes of the acinar lumen were significantly decreased (p < 0.05), by 38% and 44% respectively, which was associated with significant increases (p < 0.05) in relative and absolute volumes of interacinar stroma, whereas ventral prostate weigh was unchanged. Irrespective of animal age, ghrelin did not affect serum testosterone levels. These are the first results of ghrelin treatment effects on healthy prostate appearance, which allow us to conclude that the rat ventral prostate response to ghrelin depends on the developmental stage of animals. Our results merit further investigations and may have clinical implications, especially in the light of data on possible role of ghrelin in prostate hypertrophy and adenomas.

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Effects of chronic food restriction and treatments with leptin or ghrelin on different reproductive parameters of male rats

Cited by 42 publications

References 40 publications

The effects of streptozotocin-induced diabetes on ghrelin expression in rat testis: biochemical and immunohistochemical study

The effects of streptozotocin-induced diabetes on ghrelin expression in rat testis: biochemical and immunohistochemical study

Leptin and Its Receptor Are Expressed in the Testis and in the Epididymis of Young and Adult Pigs

Histomorphometric features of ventral prostate in different aged rats after central ghrelin treatment

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Effects of chronic food restriction and treatments with leptin or ghrelin on different reproductive parameters of male rats

Cited by 42 publications

References 40 publications

The effects of streptozotocin-induced diabetes on ghrelin expression in rat testis: biochemical and immunohistochemical study

The effects of streptozotocin-induced diabetes on ghrelin expression in rat testis: biochemical and immunohistochemical study

Leptin and Its Receptor Are Expressed in the Testis and in the Epididymis of Young and Adult Pigs

Histomorphometric features of ventral prostate in different aged rats after central ghrelin treatment

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Histomorphometric features of ventral prostate in different aged rats after central ghrelin treatment