Effects of chronic low- and high-dose ethanol intake on the nitrergic relaxations of corpus cavernosum and penile nitric oxide synthase in the rabbit

Abstract

Epidemiological evidence showed that chronic ethanol consumption is a major risk factor in the development of impotence. The present study investigated the effects of carbachol-, electrical field stimulation (EFS)-, sodium nitroprusside (SNP)-and papaverine-induced relaxant responses in the isolated corpus cavernosum tissues from rabbits submitted to an 12-week course of chronic low (5% v/v) or high ethanol intake (30% v/v). Increased carbachol-and EFS-induced relaxant responses but not SNP and papaverine, were observed in low ethanol-fed rabbits compared with controls. However, impaired carbachol-and EFSinduced relaxant responses were observed in high ethanol-fed rabbits compared with control rabbits. There were no significant differences in SNP-and papaverine-induced relaxant responses between control and high ethanol-fed rabbits. In addition, decreased neuronal nitric oxide synthase (nNOS) and endothelial NOS (eNOS) immunoreactivity in penile tissue were found in high ethanol-fed rabbits, but increased the immunoreactivity in low ethanol-fed group, compared with control group. These results suggest that alterations in nitric oxide (NO) production within the cavernous tissue in the high ethanol-fed rabbits are, at least in part, responsible for the erectile dysfunction.

Disfunção Sexual E O Uso De Drogas: Uma Análise Diagnóstica

“…Um dado que também chama a atenção no mecanismo da disfunção sexual induzida pelo álcool foi verificado por Yazir Y et al (2012), sugerindo que a redução do óxido nítrico no corpo cavernoso seria pelo menos em parte relacionada com a disfunção erétil em estudo com animais em laboratórios.…”

Yeni Nesil Oral Antikoagulanların İlaç İlaç Etkileşimleri ve Güvenliliğinin Değerlendirilebilmesi İçin Hayvan Modellerinin Geliştirilmesi, Kullanımı ve Bulunabilirliği

“…In previously published animal models, we also studied with different kind of animal species and develop animal models that are closely reflect the disease scenario similar to pathologies seen in humans (diabetes model, ageing, alcoholic animal models, polymicrobial sepsis model). We characterized the effects of different drugs in terms of their effectiveness, safety and tolerability in the Balb/c mouce, Wistar albino rats and rabbit models [20][21][22][23][24] .…”

Oxidized soluble guanylyl cyclase causes erectile dysfunction in alcoholic mice

“…There is some experimental evidence in animal models to support a causal link between alcohol and ED (Aydinoglu et al, 2008; Leite et al, 2017; Lizarte et al, 2009; Tiraboschi et al, 2021; Yazir et al, 2012). Corpora cavernosa (CC) isolated from mice, rats or rabbits chronically exposed to alcohol show an impaired relaxation to acetylcholine or other muscarinic receptor agonists which stimulate the endothelium to release nitric oxide (NO) (Aydinoglu et al, 2008; Lizarte et al, 2009; Yazir et al, 2012).…”

“…There is some experimental evidence in animal models to support a causal link between alcohol and ED (Aydinoglu et al, 2008; Leite et al, 2017; Lizarte et al, 2009; Tiraboschi et al, 2021; Yazir et al, 2012). Corpora cavernosa (CC) isolated from mice, rats or rabbits chronically exposed to alcohol show an impaired relaxation to acetylcholine or other muscarinic receptor agonists which stimulate the endothelium to release nitric oxide (NO) (Aydinoglu et al, 2008; Lizarte et al, 2009; Yazir et al, 2012). The relaxant response of CC to electrical field stimulation (EFS), which induces the release of NO from nitrergic nerves, that is, an in vitro correlate of penile erection, also has been observed to be impaired in some models of chronic exposure to alcohol (Yazir et al, 2012).…”

“…Corpora cavernosa (CC) isolated from mice, rats or rabbits chronically exposed to alcohol show an impaired relaxation to acetylcholine or other muscarinic receptor agonists which stimulate the endothelium to release nitric oxide (NO) (Aydinoglu et al, 2008; Lizarte et al, 2009; Yazir et al, 2012). The relaxant response of CC to electrical field stimulation (EFS), which induces the release of NO from nitrergic nerves, that is, an in vitro correlate of penile erection, also has been observed to be impaired in some models of chronic exposure to alcohol (Yazir et al, 2012). The effect develops after 1 week (Aydinoglu et al, 2008) to 4 weeks (Yazir et al, 2012) of treatment and is dose‐dependent (Yazir et al, 2012).…”