Effects of Ciprofloxacin Alone or in Mixture with Sulfamethoxazole on the Efficiency of Anaerobic Digestion and Its Microbial Community

Miritana, Valentina Mazzurco; Patrolecco, Luisa; Caracciolo, Anna Barra; Visca, Andrea; Piccinini, Flavia; Signorini, Antonella; Rosa, Sílvia; Grenni, Paola; Garbini, Gian Luigi; Spataro, Francesca; Rauseo, Jasmin; Massini, Giulia

doi:10.3390/antibiotics11081111

Cited by 9 publications

(2 citation statements)

References 53 publications

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“…A growing number of intensive livestock farms are turning to anaerobic digestion (AD) technology to solve the problem concerning the disposal of the huge volumes of manure and slurry produced every day. The produced biogas and digestate provide significant energy and fertilizer benefits [2]. Anaerobic digestion is an efficient process in which organic matter breaks down naturally in the absence of oxygen and takes place in a reactor, where the resulting greenhouse gases can be reused rather than released into the atmosphere [1].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Different Combinations of Cattle Organic Soil Amendments and Copper on Lettuce (cv. Rufus) Plant Growth

De Carolis,

Iori,

Narciso

et al. 2024

Environments

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In modern agricultural production, cattle manure waste recovery is considered as a sustainable approach to agricultural waste management, reducing environmental pollution and chemical fertilizer use. This study aimed to investigate the effects of manure and digestate derived from a pilot-scale livestock waste-recycling system, in combination with a low copper concentration as a fungicide, on the physiological response of lettuce cv Rufus (Lactuca sativa L.) plants and the associated soil microbiome. A five-week microcosm experiment was conducted in a greenhouse under environmental conditions. Lettuce plant performance was assessed in terms of biomass, leaf area index, photosynthetic activity, chlorophyll measurements, lipid peroxidation, total phenolic content, and nutrient uptake. The results suggested that incorporating digestate into the potting soil mix significantly enhanced crop yields compared to the control and manure treatments. The soil microbial activity increased in the presence of fertilizers, improving the soil chemical and biological properties. The addition of copper negatively affected the growth and physiological performance of the lettuce plants under both the control and manure-treated conditions, except for those grown in the presence of digestate, where copper accumulation was reduced. These findings highlight the potential of growing horticultural crops using organic fertilization through livestock waste anaerobic digestate, establishing a waste-to-food recycling system.

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Section: Introductionmentioning

confidence: 99%

The Effects of Different Combinations of Cattle Organic Soil Amendments and Copper on Lettuce (cv. Rufus) Plant Growth

De Carolis,

Iori,

Narciso

et al. 2024

Environments

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“…Currently 250 different antibiotics are registered for use in human and veterinary medicine [ 2 , 3 , 4 ]. Once administered, only a part of an AB is metabolized in a treated organism and between 10–100% of this kind of chemical can be excreted in unchanged form or as a metabolite [ 5 ]. Consequently, ABs can reach wastewater treatment plants (human origin) and natural environments (livestock origin).…”

Section: Introductionmentioning

confidence: 99%

Overview of Direct and Indirect Effects of Antibiotics on Terrestrial Organisms

Narciso,

Barra Caracciolo,

De Carolis

2023

Antibiotics

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Antibiotics (ABs) have made it possible to treat bacterial infections, which were in the past untreatable and consequently fatal. Regrettably, their use and abuse among humans and livestock led to antibiotic resistance, which has made them ineffective in many cases. The spread of antibiotic resistance genes (ARGs) and bacteria is not limited to nosocomial environments, but also involves water and soil ecosystems. The environmental presence of ABs and ARGs is a hot topic, and their direct and indirect effects, are still not well known or clarified. A particular concern is the presence of antibiotics in agroecosystems due to the application of agro-zootechnical waste (e.g., manure and biosolids), which can introduce antibiotic residues and ARGs to soils. This review provides an insight of recent findings of AB direct and indirect effects on terrestrial organisms, focusing on plant and invertebrates. Possible changing in viability and organism growth, AB bioaccumulation, and shifts in associated microbiome composition are reported. Oxidative stress responses of plants (such as reactive oxygen species production) to antibiotics are also described.

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Effects of sulfamethoxazole and copper on the natural microbial community from a fertilized soil

Narciso,

Grenni,

Spataro

et al. 2024

Appl Microbiol Biotechnol

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Cattle manure or its digestate, which often contains antibiotic residues, can be used as an organic fertilizer and copper (Cu) as a fungicide in agriculture. Consequently, both antibiotics and Cu are considered soil contaminants. In this work, microcosms were performed with soil amended with either manure or digestate with Cu and an antibiotic (sulfamethoxazole, SMX) copresence and the planting of Lactuca sativa. After the addition of the organic amendments, a prompt increase in the microbial activity and at the same time of the sul1 and intI1 genes was observed, although ARGs generally decreased over time. In the amended and spiked microcosms, the microbial community was able to remove more than 99% of SMX in 36 days and the antibiotic did not bioaccumulate in the lettuce. Interestingly, where Cu and SMX were co-present, ARGs (particularly sul2) increased, showing how copper had a strong effect on resistance persistence in the soil. Copper also had a detrimental effect on the plant-microbiome system, affecting plant biomass and microbial activity in all conditions except in a digestate presence. When adding digestate microbial activity, biodiversity and lettuce biomass increased, with or without copper present. Not only did the microbial community favour plant growth, but lettuce also positively influenced its composition by increasing bacterial diversity and classes (e.g., Alphaproteobacteria) and genera (e.g., Bacillus), thus indicating a good-quality soil. Key points• Cattle digestate promoted the highest microbial activity, diversity, and plant growth • Cattle digestate counteracted detrimental contaminant effects • Cu presence promoted antibiotic cross-resistance in soil

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Effects of Ciprofloxacin Alone or in Mixture with Sulfamethoxazole on the Efficiency of Anaerobic Digestion and Its Microbial Community

Cited by 9 publications

References 53 publications

The Effects of Different Combinations of Cattle Organic Soil Amendments and Copper on Lettuce (cv. Rufus) Plant Growth

The Effects of Different Combinations of Cattle Organic Soil Amendments and Copper on Lettuce (cv. Rufus) Plant Growth

Overview of Direct and Indirect Effects of Antibiotics on Terrestrial Organisms

Effects of sulfamethoxazole and copper on the natural microbial community from a fertilized soil

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