Effects of citric acid supplementation on rumen fermentation, urinary excretion of purine derivatives and feed digestibility in steers

Wang, Cong; Liu, Qiang; Meng, Jiao; Yang, Wude; Yang, Xiaoshan; He, Dong Chang; Kuan-hu, Dong; Huang, Yingxiang

doi:10.1002/jsfa.3724

Cited by 15 publications

(17 citation statements)

References 22 publications

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“…The CAc treatment of barley combined with the high P diet improved NDF degradation. Improvement in in situ NDF degradation due to treatment with CAc has been reported previously for steers (Wang et al, 2009). Those authors observed improved ruminal fibrolytic bacterial activity resulting in an increased ruminal acetate concentration, similar to the present study, and improved total-tract nutrient digestibility.…”

Section: Discussionsupporting

confidence: 91%

Treatment of grain with organic acids at 2 different dietary phosphorus levels modulates ruminal microbial community structure and fermentation patterns in vitro

Harder

Khol-Parisini

Metzler‐Zebeli

et al. 2015

Journal of Dairy Science

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Recent data indicate positive effects of treating grain with citric (CAc) or lactic acid (LAc) on the hydrolysis of phytate phosphorus (P) and fermentation products of the grain. This study used a semicontinuous rumen simulation technique to evaluate the effects of processing of barley with 50.25 g/L (wt/vol) CAc or 76.25 g/L LAc on microbial composition, metabolic fermentation profile, and nutrient degradation at low or high dietary P supply. The low P diet [3.1g of P per kg of dry matter (DM) of dietary P sources only] was not supplemented with inorganic P, whereas the high P diet was supplemented with 0.5 g of inorganic P per kg of DM through mineral premix and 870 mg of inorganic P/d per incubation fermenter via artificial saliva. Target microbes were determined using quantitative PCR. Data showed depression of total bacteria but not of total protozoa or short-chain fatty acid (SCFA) concentration with the low P diet. In addition, the low P diet lowered the relative abundance of Ruminococcus albus and decreased neutral detergent fiber (NDF) degradation and acetate proportion, but increased the abundance of several predominantly noncellulolytic bacterial species and anaerobic fungi. Treatment of grain with LAc increased the abundance of total bacteria in the low P diet only, and this effect was associated with a greater concentration of SCFA in the ruminal fluid. Interestingly, in the low P diet, CAc treatment of barley increased the most prevalent bacterial group, the genus Prevotella, in ruminal fluid and increased NDF degradation to the same extent as did inorganic P supplementation in the high P diet. Treatment with either CAc or LAc lowered the abundance of Megasphaera elsdenii but only in the low P diet. On the other hand, CAc treatment increased the proportion of acetate in the low P diet, whereas LAc treatment decreased this variable at both dietary P levels. The propionate proportion was significantly increased by LAc at both P levels, whereas butyrate increased only with the low P diet. Treatments with CAc or LAc reduced the degradation of CP and ammonia concentration compared with the control diet at both P levels. In conclusion, the beneficial effects of CAc and LAc treatment on specific ruminal microbes, fermentation profile, and fiber degradation in the low P diet suggest the potential for the treatment to compensate for the lack of inorganic P supplementation in vitro. Further research is warranted to determine the extent to which the treatment can alleviate the shortage of inorganic P supplementation under in vivo conditions.

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Section: Discussionsupporting

confidence: 91%

Treatment of grain with organic acids at 2 different dietary phosphorus levels modulates ruminal microbial community structure and fermentation patterns in vitro

Harder

Khol-Parisini

Metzler‐Zebeli

et al. 2015

Journal of Dairy Science

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“…Feeding trials also confirmed that malic acid had a positive effect on increasing milk production in dairy cows (Sniffen et al, 2006;Devant et al, 2007;Wang et al, 2009b) and improving beef cattle performance (Martin et al, 1999;Castillo et al, 2007). Citric acid is another essential intermediate in the citric acid cycle, which has a similar function in stimulating rumen fermentation and improving animal performance (Packett and Butcher, 1963;Sun et al, 2008;Wang et al, 2009a).…”

Section: Introductionmentioning

confidence: 84%

“…In the present study, the lower pH, greater lactic acid, and lower acetic acid concentrations in silages treated with malic and citric acids (at application rates of 0.1 or 0.5% of FW) indicate that including malic or citric acids at ensiling could improve or alter the fermentation process of the ensiled alfalfa. These acids were also reported to stimulate in vitro mixed ruminal microorganism fermentation (Martin and Streeter, 1995;Carro and Ranilla, 2003;Wang et al, 2009a). Nevertheless, when the application level of these 2 acids reached 1% of FW, remarkable declines in the lactic acid concentration and the number of LAB were observed, which suggests that the growth and fermentation of LAB were inhibited by the high concentration of malic or citric acids in ensiled forage.…”

Section: Discussionmentioning

confidence: 99%

Effects of addition of malic or citric acids on fermentation quality and chemical characteristics of alfalfa silage

Ding

et al. 2017

Journal of Dairy Science

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We studied the effects on alfalfa preservation and chemical composition of the addition of different levels of malic acid and citric acid at ensiling as well as the utilization efficiency of these 2 organic acids after fermentation. Alfalfa was harvested at early bloom stage. After wilting to a dry matter content of approximately 40%, the alfalfa was chopped into 1- to 2-cm pieces for ensiling. Four levels (0, 0.1, 0.5, and 1% of fresh weight) of malic acid or citric acid were applied to chopped alfalfa at ensiling with 4 replicates for each treatment, and the treated alfalfa forages were ensiled for 60 d in vacuum-sealed polyethylene bags (dimensions: 200 mm × 300 mm) packed with 200 to 230 g of fresh alfalfa per mini silo and an initial density of 0.534 g/cm. The application of malic or citric acids at ensiling for 60 d led to lower silage pH than was observed in the control silage (0% of malic or citric acids). Application of the 2 organic acids led to higher lactic acid concentration in alfalfa silage than in the control silage except with the application rate of 1% of fresh weight. Silages treated with both organic acids had lower nonprotein nitrogen concentrations than the control silages, and the nonprotein nitrogen concentrations in ensiled forages decreased with the increase in malic or citric acid application rates. The application of the 2 organic acid additives led to lower saturated fatty acid proportions and higher polyunsaturated fatty acid proportions in ensiled alfalfa than in the control silage. The amount of malic and citric acids degraded during ensiling of alfalfa was 1.45 and 0.63 g, respectively. At the application rate of 0.5% of fresh weight, residues of malic acid and citric acid in alfalfa silage were 11.1 and 13.6 g/kg of dry matter. These results indicate that including malic or citric acids at the ensiling of alfalfa effectively improved silage fermentation quality, limited proteolysis, improved fatty acid composition of the ensiled forage, and could provide animals with additional feed additives proven to promote animal performance. However, when the application rate of both organic acids reached 1%, the concentration of lactic acid in silages decreased notably. Additionally, 0.5 and 1% application rates also increased the yeast count in ensiled alfalfa.

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“…This suggests that the larger amounts of lactic acid and glycerol in CBDS increased its ability to produce propionate and butyrate in the rumen compared with soybean meal. Although the concentration of acetic acid in the rumen increased linearly with increasing citric acid supplementation, the concentrations of propionate and butyrate in the rumen did not increase (Wang et al ). In the present study, the intake of citric acid from soybean was lower than the lowest amount of citric acid supplementation in the study of Wang et al ().…”

Section: Resultsmentioning

confidence: 99%

“…Although the concentration of acetic acid in the rumen increased linearly with increasing citric acid supplementation, the concentrations of propionate and butyrate in the rumen did not increase (Wang et al ). In the present study, the intake of citric acid from soybean was lower than the lowest amount of citric acid supplementation in the study of Wang et al (). Therefore, citric acid in soybean meal might not have increased the concentration of acetic acid in the Soybean‐t group.…”

Section: Resultsmentioning

confidence: 99%

Preliminary study of the effects of condensed barley distillers soluble on rumen fermentation and plasma metabolites in Japanese Black cows

Tsuruoka

Kanamaru

Takahashi

et al. 2016

Animal Science Journal

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In Japan, condensed barley distillers soluble (CBDS) is a widely known liquor byproduct that contains a high level of protein and is used as a supplementary protein feed for cattle. The present study evaluated the effects of CBDS feed on rumen fermentation and plasma metabolites in Japanese Black cows. Applying a replicated 3 × 3 Latin square design, nine cows were offered CBDS and hay (CBDS-t), soy bean meal and hay (Soybean-t) and only hay (Hay-t) over 35 days. We collected ruminal fluid and plasma just before feeding and at 3 h after feeding. The concentrations of propionate and butyrate in the rumen before feeding were lower in the CBDS-t than in the Soybean-t group (P < 0.05). However, after 3 h, the concentrations were higher in the CBDS-t than in the Soybean-t and Hay-t groups (P < 0.05). Although, there were no differences in the compositions (% mol) of propionate and butyrate in the rumen and the concentration of plasma β-hydroxybutyric acid before feeding between treatments, after 3 h they were significantly higher in the CBDS-t than in the Soybean-t and Hay-t groups (P < 0.05). These results indicate that feeding CBDS promotes rumen fermentation and butyrate metabolism.

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Effects of citric acid supplementation on rumen fermentation, urinary excretion of purine derivatives and feed digestibility in steers

Cited by 15 publications

References 22 publications

Treatment of grain with organic acids at 2 different dietary phosphorus levels modulates ruminal microbial community structure and fermentation patterns in vitro

Treatment of grain with organic acids at 2 different dietary phosphorus levels modulates ruminal microbial community structure and fermentation patterns in vitro

Effects of addition of malic or citric acids on fermentation quality and chemical characteristics of alfalfa silage

Preliminary study of the effects of condensed barley distillers soluble on rumen fermentation and plasma metabolites in Japanese Black cows

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