Effects of clays used as oil adsorbents in lamb diets on fatty acid composition of abomasal digesta and meat

Oliveira, Maria Alexandra; Alves, Susana P.; Santos‐Silva, José; Bessa, Rui J. B.

doi:10.1016/j.anifeedsci.2016.01.006

Cited by 43 publications

(21 citation statements)

References 28 publications

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“…One of the consequences of the t-10 shift in ruminants is the low concentration of c-9,t-11-18:2 in tissues (Bravo-Lamas et al, 2016;Oliveira et al, 2016Oliveira et al, , 2017. Consistently, we observed a relatively low concentration of c-9,t-11-18:2 in LM and s.c. fat under all treatments.…”

Section: Discussionsupporting

confidence: 87%

“…Fatty acid methyl esters of feed lipids were prepared by 1-step extraction using 10% HCl in methanol and 19:0 as an internal standard (Sukhija and Palmquist, 1988). Muscle and s.c. fat lipids were extracted with dichoromethane:methanol (2:1, vol/vol) from freezedried tissue samples and transesterified into fatty acid methyl esters using a combined basic and acid catalysis as described by Oliveira et al (2016). Fatty acid methyl esters were analyzed by gas chromatography coupled with flame ionization detection (Gc-FId; Shimadzu GC-2010 Plus; Shimadzu Corp., Kyoto, Japan) using a 100% cyanopropyl polysiloxane capillary column (SP- 2560; 100 m, 0.25 mm i.d., and 0.20-µm film thickness; Supelco Inc., Bellefonte, PA).…”

Section: Fatty Acid Analysismentioning

confidence: 99%

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The reduction of starch in finishing diets supplemented with oil does not prevent the accumulation of trans-10 18:1 in lamb meat1

Costa

Alves

Francisco

et al. 2017

Journal of Animal Science

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The experiment was conducted to test the hypothesis that the replacement of cereal with low-starch feed ingredients in lambs' finishing diets supplemented with oils could prevent the accumulation of -10-18:1 in meat. Forty lambs were fed 1 of 4 diets supplemented with soybean oil (5.9%) and fish oil (1%) for 6 wk before slaughter. The control (CON) diet contained 43% barley, and in the other diets, barley was completely replaced by dehydrated citrus pulp (DCP), dehydrated sugar beet pulp (DBP), or soybean hulls (SH). Growth performance, feed intake, and carcass and meat quality traits were analyzed. At slaughter, LM samples were collected for gene expression evaluation, and 3 d after slaughter, LM and subcutaneous (s.c.) fat samples were collected for fatty acid analysis. None of the diets affected meat quality, but the DCP diet reduced ADG ( < 0.05) and the DCP and SH diets decreased the feed-to-gain ratio ( < 0.01). The DCP diet increased ( < 0.05) the risk of parakeratosis and the severity of the lesions. Moreover, the DBP treatment led to increased a* (redness) and b* (yellowness) in s.c. fat compared with the CON treatment ( < 0.05). The lipid content of LM did not differ ( > 0.05) with treatment and averaged 34.4 g/kg of meat. Diets had no effect ( > 0.05) on SFA, PUFA, and -MUFA sums and on the -6:-3 ratio in both LM and s.c. fat. A lower expression of fatty acid synthase (FASN) was found with the DCP treatment than with the other treatments ( < 0.001). All treatments showed a high accumulation of -10-18:1, averaging 91 mg/g fatty acid in LM and 147 mg/g fatty acid in s.c. fat. The concentration of -11-18:1 in the tissues was considerably lower than that of -10-18:1, and thus the -10-18:1:-11-18:1 ratio was above 3 with all treatments. Despite this, the SH diet clearly promoted a larger deposition of -11-18:1 and -9,-11-18:2 in tissues compared with the other treatments. () gene expression and SCD activity index in LM were reduced with the SH diet compared with the CON and DCP diets. Overall, these results clearly showed, for the first time, that low-starch/high-NDF diets are not able to prevent the establishment of -10 shifted rumen biohydrogenation pathways, evaluated by the deposition of biohydrogenation intermediates in lamb meat and fat.

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Section: Discussionsupporting

confidence: 87%

Section: Fatty Acid Analysismentioning

confidence: 99%

The reduction of starch in finishing diets supplemented with oil does not prevent the accumulation of trans-10 18:1 in lamb meat1

Costa

Alves

Francisco

et al. 2017

Journal of Animal Science

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“…Feed chemical composition was determined using routine and widespread methods described previously . VFA were directly analysed by gas chromatography with flame ionization detection (GC‐FID) in a Shimadzu GC‐2010 Plus chromatograph (Kyoto, Japan) equipped with a Nukol capillary column (30 m, 0.25 mm i.d., 0.25 µm film thickness; Supelco Inc., Bellefonte, PA, USA) as described in the literature . Freeze‐dried rumen samples were transesterified into FA methyl esters by using a combined basic followed by acid catalysis .…”

Section: Methodsmentioning

confidence: 99%

“…The internal standard was 19:0 (1 mg mL −1 ). FA methyl esters were separated by GC‐FID using a Shimadzu GC‐2010 Plus chromatograph equipped with a TR‐CN100 silica capillary column (100 m, 0.25 mm i.d., 0.20 µm film thickness; Tecknokroma, Barcelona, Spain) according to procedures described in the literature . Identification of FA methyl esters and dimethylacetals (DMA) was achieved by comparison of retention times with those of authentic standards (37 Component FAME Mix, Supelco Inc.; Bacterial FAME Mix, Matreya LLC, Pleasant Gap, PA, USA) and by confirmation with gas chromatography/mass spectrometry (GC/MS) in a Shimadzu GC/MS QP 2010 Plus chromatograph …”

Section: Methodsmentioning

confidence: 99%

Modulation ofin vitrorumen biohydrogenation byCistus ladanifertannins compared with other tannin sources

Costa

Alves

Cabo

et al. 2016

J. Sci. Food Agric.

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“…The rates of reduction in C18:3n-3 and cis-9 C18:1 from dietary values (d 61-90) to rumen values, were considered to reflect hydrogenation [6,[22][23][24]. The rate of increment in the proportion of C18:0 from diet (d 61-90) to rumen was considered to reflect synthesis.…”

Section: Discussionmentioning

confidence: 99%

Palm oil protects α-linolenic acid from rumen biohydrogenation and muscle oxidation in cashmere goat kids

Wang

Martin

Wen

et al. 2020

J Animal Sci Biotechnol

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Background In ruminants, dietary C18:3n-3 can be lost through biohydrogenation in the rumen; and C18:3n-3 that by-passes the rumen still can be lost through oxidation in muscle, theoretically reducing the deposition of C18:3n-3, the substrate for synthesis of poly-unsaturated fatty acids (n-3 LCPUFA) in muscle. In vitro studies have shown that rumen hydrogenation of C18:3n-3 is reduced by supplementation with palm oil (rich in cis-9 C18:1). In addition, in hepatocytes, studies with neonatal rats have shown that cis-9 C18:1 inhibits the oxidation of C18:3n-3. It therefore seems likely that palm oil could reduce both rumen biohydrogenation of C18:3n-3 and muscle oxidation of C18:3n-3. The present experiment tested whether the addition of palm oil to a linseed oil supplement for goat kids would prevent the losses of C18:3n-3 and thus improve the FA composition in two muscles, Longissimus dorsi and Biceps femoris. To investigate the processes involved, we studied the rumen bacterial communities and measured the mRNA expression of genes related to lipid metabolism in Longissimus dorsi. Sixty 4-month-old castrated male Albas white cashmere kids were randomly allocated among three dietary treatments. All three diets contained the same ingredients in the same proportions, but differed in their fat additives: palm oil (PMO), linseed oil (LSO) or mixed oil (MIX; 2 parts linseed oil plus 1 part palm oil on a weight basis). Results Compared with the LSO diet, the MIX diet decreased the relative abuandance of Pseudobutyrivibrio, a bacterial species that is positively related to the proportional loss rate of dietary C18:3n-3 and that has been reported to generate the ATP required for biohydrogenation (reflecting a decrease in the abundance of rumen bacteria that hydrogenate C18:3n-3 in MIX kids). In muscle, the MIX diet increased concentrations of C18:3n-3, C20:5n-3, C22:6n-3, and n-3 LCPUFA, and thus decreased the n-6/n-3 ratio; decreased the mRNA expression of CPT1β (a gene associated with fatty acid oxidation) and increased the mRNA expression of FADS1 and FADS2 (genes associated with n-3 LCPUFA synthesis), compared with the LSO diet. Interestingly, compared to Longissimus dorsi, Biceps femoris had greater concentrations of PUFA, greater ratios of unsaturated fatty acids/saturated fatty acids (U/S), and poly-unsaturated fatty acids/saturated fatty acids (P/S), but a lesser concentration of saturated fatty acids (SFA). Conclusions In cashmere goat kids, a combination of linseed and palm oils in the diet increases the muscle concentration of n-3 LCPUFA, apparently by decreasing the relative abundance of rumen bacteria that are positively related to the proportional loss rate of dietary C18:3n-3, by inhibiting mRNA expression of genes related to C18:3n-3 oxidation in muscle, and by up-regulating mRNA expression of genes related to n-3 LCPUFA synthesis in muscle, especially in Longissimus dorsi.

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Effects of clays used as oil adsorbents in lamb diets on fatty acid composition of abomasal digesta and meat

Cited by 43 publications

References 28 publications

The reduction of starch in finishing diets supplemented with oil does not prevent the accumulation of trans-10 18:1 in lamb meat1

The reduction of starch in finishing diets supplemented with oil does not prevent the accumulation of trans-10 18:1 in lamb meat1

Modulation ofin vitrorumen biohydrogenation byCistus ladanifertannins compared with other tannin sources

Palm oil protects α-linolenic acid from rumen biohydrogenation and muscle oxidation in cashmere goat kids

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