Effects of clothianidin exposure on sperm quality, testicular apoptosis and fatty acid composition in developing male rats

Bal, Ramazan; Türk, Gaffari; Yılmaz, Ökkeş; Etem, Ebru Önalan; Kuloğlu, Tuncay; Baydaş, Gıyasettin; Nazıroğlu, Mustafa

doi:10.1007/s10565-012-9215-0

Cited by 47 publications

(44 citation statements)

References 62 publications

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“…In male rats, neonicotinoid decreased sperm motility and epididymal sperm concentration, and increased the abnormal sperm rate [2]. We found that it decreased the number of germ cells and increased DNA-fragmented germ cells in seminiferous epithelium in a dose-dependent manner, but we also found normal spermatogenesis in the treatment groups and little effect on the egg weights and the fertilization rates in females bred with the treated males.…”

Section: Discussionmentioning

confidence: 47%

“…It is reported that neonicotinoid causes DNA fragmentation of rat sperm through oxidative stress [2], and the injection of DNA-fragmented sperm into human egg cells inhibits embryo development after the eight-cell stage [18]. In addition, it is known that injury to sperm DNA after second meiosis is not repaired, and that DNA-fragmented sperm are found in human ejaculate.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, several studies have reported that the breakdown products of neonicotinoids are toxic to mammals [4,19], and they affect the reproductive systems of male mice [22] and rats [2,12] through oxidative stress. Thus, it is suggested that neonicotinoids are harmful to mammals including human.…”

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confidence: 99%

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Effects of Exposure to Clothianidin on the Reproductive System of Male Quails

et al. 2013

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ABSTRACT. Clothianidin (CTD) is a neonicotinoid developed in the 1990s as an insecticide having selective toxicity, but it was later found to cause reproductive abnormalities in rats through oxidative stress. There is an attempt to preserve endangered animals, including the Japanese crested ibis, in Japan. However, there is a concern that neonicotinoid affects the reproduction of this bird, since it is used in its habitat. CTD toxicity in the birds is poorly understood, so we investigated whether or not the daily oral administration of CTD has any deleterious effects on the reproductive functions of mature male quails as experimental animals. The animals were randomly divided into four groups of 6 or 7 quails each, treated orally with 0, 0.02, 1 or 50 mg CTD/kg body weight (Control, CTD0.02, CTD1 and CTD50). After that the males bred with untreated females to estimate the egg weights, and rates of fertilization and normal development, the testes, liver and spleen were examined histologically. Vacuolization and the number of germ cells having fragmented DNA in seminiferous tubules, and the number and size of vacuoles in hepatocytes increased dose-dependently. There were no significant differences in egg weights and fertilization rates between the groups, but some eggs of the CTD1 and CTD50 groups failed to develop, and embryonic length decreased dose-dependently. Thus, it was found that CTD affected the reproduction of the male quail through the fragmentation of germ cells and the inhibition or delay of embryonic development.

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Section: Discussionmentioning

confidence: 47%

Section: Discussionmentioning

confidence: 99%

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Effects of Exposure to Clothianidin on the Reproductive System of Male Quails

et al. 2013

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“…For example; Tanaka (2012) has reported that CTD administration through diet to female mice during the gestation period has no effect on selected reproductive parameters, including litter size, litter weight, or sex ratio at birth. However, in our previous study (Bal et al, 2012a), we demonstrated that CTD exposure at the no observed adverse effect level (NOAEL) dose (32 mg/kg) daily for 90 days caused significant decreases in reproductive organ weights, sperm concentration, testicular GSH level, and significant increases in some testicular fatty acid composition, cholesterol level, apoptotic germ cells, and sperm DNA fragmentation in developing male rats. In our other study (Bal et al, 2012b), we also found that the nicotinoid, imidacloprid, when administered at NOAEL dose levels, leads to testicular dysfunction, including deteriorated sperm quality, decreased testosterone level, increased apoptotic germ cells, increased sperm DNA fragmentation, and disturbed oxidant/antioxidant balance and fatty acid composition in adult male rats.…”

Section: Clothianidinmentioning

confidence: 87%

Effects of the neonicotinoid insecticide, clothianidin, on the reproductive organ system in adult male rats

Bal¹,

Türk²,

Tuzcu³

et al. 2013

Drug and Chemical Toxicology

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Clothianidin (CTD) is a novel, broad-spectrum insecticide. In the current study, it was aimed to study the effect of subchronic exposure to low doses of CTD (2, 8 and 24 mg/kg body weight/day) on the reproductive system in adult rats. CTD treatment did not significantly change serum testosterone level or sperm parameters (e.g. concentration, motility and morphology), but caused significant decreases in weights of epididymis, right cauda epididymis and seminal vesicles. CTD treatment did not cause sperm DNA fragmentation and did not change the apoptotic index in the seminiferous tubules and levels of α-tocopherol and glutathione, but increased the level of thiobarbituric acid-reactive substances and cholesterol levels significantly at all doses. CTD exposure caused significant elevations in palmitic, linoleic and arachidonic acids in testis in all CTD-exposed groups. There was a drop in 20:4/18:2 (arachidonic acid/linoleic acid) ratio and an increase in 18:1n-9/18:0 (oleic acid/stearic acid) ratios in all CTD groups, in comparison to the control group. In conclusion, CTD had little detectable detrimental effects on the reproductive system of male rats over the measured parameters.

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“…However, studies (8,9) have reported genotoxic effects for Acm and other neonicotinoid insecticides (30)(31)(32)(33). For instance, Bal et al (34) reported that the neonicotinoid clothianidin induced impairment of the male mouse reproductive system. Such claims are inconsistent with the results of this study.…”

Section: Discussionmentioning

confidence: 99%

Abnormal sperm morphology in mouse germ cells after short-term exposures to acetamiprid, propineb, and their mixture

Rasgele

2014

Archives of Industrial Hygiene and Toxicology

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Pesticides are one of the most potent environmental contaminants, which accumulate in biotic and abiotic components of ecosystems. Acetamiprid (Acm), a neonicotinoid insecticide, and Propineb (Pro), a dithiocarbamate fungicide, are widely used to control sucking insects and fungal infections on crops, respectively. The present study was undertaken to investigate the genotoxic effects of these compounds, individually and in mixtures, in mouse germ cells by using the sperm morphology assay. Mice were injected intraperitoneally with 0.625, 1.25, and 2.50 µg mL -1 of Acm, 12.5, 25, and 50 µg mL -1 of Pro, and their mixture at the same concentrations over 24 and 48 h. Acm did not significantly increase the percentage of abnormal sperm at any concentration. The frequency of abnormal sperm significantly increased after 24 and 48 h of exposure to 50 µg mL -1 of Pro. The mixtures of 2.50 µg mL -1 of Acm and 50 µg mL -1 of Pro induced sperm abnormalities antagonistically both after 24 and 48 h of exposure. Results suggest that Acm was non-genotoxic for mouse germ cells, while Pro may have been a germ cell mutagen due to the observed increase in the frequency of sperm abnormalities. However, to gain better insight into the mutagenicity and DNA damaging potential of both of these pesticides, further studies at molecular level should be done.

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Effects of clothianidin exposure on sperm quality, testicular apoptosis and fatty acid composition in developing male rats

Cited by 47 publications

References 62 publications

Effects of Exposure to Clothianidin on the Reproductive System of Male Quails

Effects of Exposure to Clothianidin on the Reproductive System of Male Quails

Effects of the neonicotinoid insecticide, clothianidin, on the reproductive organ system in adult male rats

Abnormal sperm morphology in mouse germ cells after short-term exposures to acetamiprid, propineb, and their mixture

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