Effects of CO2 Narcosis on the Onset of Oviposition and Colony Founding Success of Post Diapausing Bumblebee (Bombus terrestris) Queens

Karsli, Bahar Argun; Gürel, Fehmi

doi:10.9775/kvfd.2012.7576

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…However, the time between mating and CO 2 narcosis does not seem to matter or to affect egg-laying success in B. impatiens. This is in line with previous work in B. terrestris showing successful initiation of a colony following CO 2 treatment, despite modifications in the number of CO 2 applications [34], the duration of CO 2 exposure, and the timing to administrate the CO 2 treatment (i.e., 5 vs. 20-30 d after mating) [33,39], suggesting that there is a fair amount of flexibility in the way CO 2 is applied. More importantly, these results emphasize that queens can achieve high rates of survival and egg laying following CO 2 narcosis, both of which can be drawbacks of using cold storage [55,56].…”

Section: Discussionsupporting

confidence: 90%

“…For example, B. hypocrita queens kept in cold storage (5 • C, 4 months) preferred dry vermiculite in place of soil, while B. ignitus queens preferred moist (50-55% moisture) vermiculite or peat moss [32]. CO 2 narcosis has been shown to induce a transition to reproduction in B. impatiens, B. hypnorum, B. pratorum, B. terrestris, and B. vestalis [8,[33][34][35]. Thus, its mode of action may be similar across species, but this has been poorly studied in non-commercially available species.…”

Section: Introductionmentioning

confidence: 99%

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Optimizing Laboratory Rearing of a Key Pollinator, Bombus impatiens

Treanore

Barie

Derstine

et al. 2021

Insects

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Bumble bees are key pollinators for wild and managed plants and serve as a model system in various research fields, largely due to their commercial availability. Despite their extensive use, laboratory rearing of bumble bees is often challenging, particularly during the solitary phase queens undergo before founding a colony. Using a literature survey, we demonstrate that most studies rely on commercially available species that are provided during the colony’s social phase, limiting study on early phases of the life cycle and the ability to control for colony age and relatedness. Laboratory rearing is challenging since the queen solitary phase is less understood compared to the social phase. To overcome this barrier, we examined several aspects related to the queen solitary phase: the effect of age on likelihood of mating, how the timing of CO2 narcosis post-mating (a technique to bypass diapause) affects egg-laying, and whether different social cues affect the success of colony initiation. Our data show an optimum age for mating in both sexuals and decreased egg-laying latency in the presence of workers and pupae. The timing of CO2 narcosis did not significantly affect egg laying in queens. These findings can be incorporated to improve bumble bee rearing for research purposes.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Optimizing Laboratory Rearing of a Key Pollinator, Bombus impatiens

Treanore

Barie

Derstine

et al. 2021

Insects

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“…The four colonies were divided into thirty queenless microcolonies by placing five bumblebee female workers from the same original colony in rectangular plastic boxes (L 16.8 cm × H 6.6 cm × W 11.6 cm) with a stainless-steel mesh lid for ventilation, a 10-ml syringe containing syrup (sucrose solution), and pollen feeders. Microcolonies were kept in a dark room with red light at ambient temperature (24 °C) and ~50% humidity (VicTsing Ultrasonic Humidifier) ( Gurel & Gosterit, 2008 ).…”

Section: Methodsmentioning

confidence: 99%

Heatwave-like events affect drone production and brood-care behaviour in bumblebees

Sepúlveda,

Nicholls,

Schuett

et al. 2024

PeerJ

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Climate change is currently considered one of the major threats to biodiversity and is associated with an increase in the frequency and intensity of extreme weather events, such as heatwaves. Heatwaves create acutely stressful conditions that may lead to disruption in the performance and survival of ecologically and economically important organisms, such as insect pollinators. In this study, we investigated the impact of simulated heatwaves on the performance of queenless microcolonies of Bombus terrestris audax under laboratory conditions. Our results indicate that heatwaves can have significant impacts on bumblebee performance. However, contrary to our expectations, exposure to heatwaves did not affect survival. Exposure to a mild 5-day heatwave (30–32 °C) resulted in increased offspring production compared to those exposed to an extreme heatwave (34–36 °C) and to the control group (24 °C). We also found that brood-care behaviours were impacted by the magnitude of the heatwave. Wing fanning occurred occasionally at temperatures of 30–32 °C, whereas at 34–36 °C the proportion of workers engaged in this thermoregulatory behaviour increased significantly. Our results provide insights into the effects of heatwaves on bumblebee colony performance and underscore the use of microcolonies as a valuable tool for studying the effects of extreme weather events. Future research, especially field-based studies replicating natural foraging conditions, is crucial to complement laboratory-based studies to comprehend how heatwaves compromise the performance of pollinators. Such studies may potentially help to identify those species more resilient to climate change, as well as those that are most vulnerable.

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“…Bombus terrestris colonies were reared under standard laboratory conditions (27 ± 2°C, 60 ± 5% relative humidity; Gurel and Karsli, 2013). Fresh pollen and sugar solutions (1:1, w/v) were provided ad libitum as a diet (Zhang et al, 2021).…”

Section: Sample Collectionmentioning

confidence: 99%

Gut microbiota is a potential factor in shaping phenotypic variation in larvae and adults of female bumble bees

Guo

Jiao²,

Ding³

et al. 2023

Front. Microbiol.

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Host symbionts are often considered an essential part of the host phenotype, influencing host growth and development. Bumble bee is an ideal model for investigating the relationship between microbiota and phenotypes. Variations in life history across bumble bees may influence the community composition of gut microbiota, which in turn influences phenotypes. In this study, we explored gut microbiota from four development stages (early-instar larvae, 1st instar; mid-instar larvae, 5th instar; late-instar larvae, 9th instar; and adults) of workers and queens in the bumble bee Bombus terrestris using the full-length 16S rRNA sequencing technology. The results showed that morphological indices (weight and head capsule) were significantly different between workers and queens from 5th instar larvae (p < 0.01). The alpha and beta diversities of gut microbiota were similar between workers and queens in two groups: early instar and mid instar larvae. However, the alpha diversity was significantly different in late instar larvae or adults. The relative abundance of three main phyla of bacteria (Cyanobacteria, Proteobacteria, and Firmicutes) and two genera (Snodgrassella and Lactobacillus) were significantly different (p < 0.01) between workers and queens in late instar larvae or adults. Also, we found that age significantly affected the microbial alpha diversity as the Shannon and ASVs indices differed significantly among the four development stages. Our study suggests that the 5th instar larval stage can be used to judge the morphology of workers or queens in bumble bees. The key microbes differing in phenotypes may be involved in regulating phenotypic variations.

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Effects of CO2 Narcosis on the Onset of Oviposition and Colony Founding Success of Post Diapausing Bumblebee (Bombus terrestris) Queens

Cited by 5 publications

References 12 publications

Optimizing Laboratory Rearing of a Key Pollinator, Bombus impatiens

Optimizing Laboratory Rearing of a Key Pollinator, Bombus impatiens

Heatwave-like events affect drone production and brood-care behaviour in bumblebees

Gut microbiota is a potential factor in shaping phenotypic variation in larvae and adults of female bumble bees

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