Effects of cofactors RIC-3, TMX3 and UNC-50, together with distinct subunit ratios on the agonist actions of imidacloprid on Drosophila melanogaster Dα1/Dβ1 nicotinic acetylcholine receptors expressed in Xenopus laevis oocytes

Takayama, Koichi; Ito, Ryo; Yamamoto, Hirosuke; Otsubo, Shuya; Matsumoto, Rei; Ojima, Hisanori; Komori, Yuma; Matsuda, Kazuhiko; Ihara, Makoto

doi:10.1016/j.pestbp.2022.105177

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…laevis oocytes, which represent the first instance of recombination with the α and β subunits of agricultural pests (thrips). Previous studies have shown that altering injection ratios or coinjecting with α3, α4, and β3 can lead to the formation of nAChRs with distinct functions. , However, it is worth noting that neonicotinoids are predicted to target the α1, α2, β1, and β2 subunits of insect nAChRs . Therefore, the homozygous expression of these receptors can serve as a valuable screening tool for the discovery of novel insecticidal compounds.…”

Section: Discussionmentioning

confidence: 99%

Mutation V65I in the β1 Subunit of the Nicotinic Acetylcholine Receptor Confers Neonicotinoid and Sulfoxaflor Resistance in Insects

Zhang,

Chen,

Chen

et al. 2024

J. Agric. Food Chem.

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Neonicotinoids have been widely used to control pests with remarkable effectiveness. Excessive insecticides have led to serious insect resistance. Mutations of the nicotinic acetylcholine receptor (nAChR) are one of the reasons for neonicotinoid resistance conferred in various agricultural pests. Two mutations, V65I and V104I, were found in the nAChR β1 subunit of two neonicotinoid-resistant aphid populations. However, the specific functions of the two mutations remain unclear. In this study, we cloned and identified four nAChR subunits (α1, α2, α8, and β1) of thrips and found them to be highly homologous to the nAChR subunits of other insects. Subsequently, we successfully expressed two subtypes nAChR (α1/α2/α8/β1 and α1/α8/β1) by coinjecting three cofactors for the first time in thrips, and α1/α8/β1 showed abundant current rapidly. Acetylcholine, neonicotinoids, and sulfoxaflor exhibited different activation capacities for the two subtypes of nAChRs. Finally, V65I was found to significantly reduce the binding ability of nAChR to neonicotinoids and sulfoxaflor through electrophysiology and computer simulations. V104I caused a decrease in agonist affinity (pEC50) but an increase in the efficacy (I max) of nAChR against neonicotinoids and reduced the binding ability of nAChR to sulfoxaflor. This study provides theoretical and technical support for studying the molecular mechanisms of neonicotinoid resistance in pests.

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Section: Discussionmentioning

confidence: 99%

Mutation V65I in the β1 Subunit of the Nicotinic Acetylcholine Receptor Confers Neonicotinoid and Sulfoxaflor Resistance in Insects

Zhang,

Chen,

Chen

et al. 2024

J. Agric. Food Chem.

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“…Subsequently, we performed differentially expressed circ_0003259 and circ_0012128 for ceRNA mechanism validation. The source genes of circ_0003259 and circ_0012128 are two genes, TRPM3 and RIC3 , respectively, both of which may be involved in the reproductive process of animals [ 47 , 48 ]. FGFR1 is a tyrosine kinase receptor that can regulate embryonic development by binding to fibroblast growth factor and then activating a series of downstream pathways [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Differentially Expressed circRNAs Associated with Fecundity in Small-Tail Han Sheep Thyroid with Different FecB Genotypes

Chang,

He,

et al. 2023

Animals

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Litter size is an economically important trait in sheep, and it is a complex trait controlled by multiple genes in multiple organs. Among them, the regulation of lamb number trait by the thyroid gland is a very important part. However, the molecular mechanisms of the thyroid gland in sheep reproduction remain unclear. Here, RNA-seq was used to detect transcriptome expression patterns in the thyroid gland between follicular phase (FP) and luteal phase (LP) in FecB BB (MM) and FecB ++ (ww) STH sheep, respectively, and to identify differentially expressed circRNAs (DECs) associated with reproduction. Bioinformatic analysis of the source genes of these DECs revealed that they can be enriched in multiple signaling pathways involved in the reproductive process of animals. We found that the source genes of these DECs, such as GNAQ, VEGFC, MAPK1, STAT1, and HSD17B7, may play important roles in the reproductive process of animals. To better understand the function of these DECs, we constructed circRNA–miRNA co-expression networks. Dual luciferase reporter assays suggested that a ceRNA regulatory mechanism between circ_0003259-oar-miR-133-TXLNA and circ_0012128-oar-miR-370-3p-FGFR1 may hold. All of these DEC expression profiles in the thyroid gland provide a novel resource for elucidating the regulatory mechanisms underlying STH sheep prolificacy.

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“…In mice, it was shown that RIC-3 expression and splicing can regulate the functional expression of nicotinic acetylcholine receptors [79]. Recently, the effects of cofactors RIC-3, TMX3, and UNC-50 on D. melanogaster Dα1/Dβ1 nicotinic acetylcholine receptors were analysed [80].…”

Section: Discussionmentioning

confidence: 99%

Generation of Mutants from the 57B Region of Drosophila melanogaster

Steinmetz,

Noh,

Klöppel

et al. 2023

Genes

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The 57B region of Drosophila melanogaster includes a cluster of the three homeobox genes orthopedia (otp), Drosophila Retinal homeobox (DRx), and homeobrain (hbn). In an attempt to isolate mutants for these genes, we performed an EMS mutagenesis and isolated lethal mutants from the 57B region, among them mutants for otp, DRx, and hbn. With the help of two newly generated deletions from the 57B region, we mapped additional mutants to specific chromosomal intervals and identified several of these mutants from the 57B region molecularly. In addition, we generated mutants for CG15651 and RIC-3 by gene targeting and mutants for the genes CG9344, CG15649, CG15650, and ND-B14.7 using the CRISPR/Cas9 system. We determined the lethality period during development for most isolated mutants. In total, we analysed alleles from nine different genes from the 57B region of Drosophila, which could now be used to further explore the functions of the corresponding genes in the future.

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Effects of cofactors RIC-3, TMX3 and UNC-50, together with distinct subunit ratios on the agonist actions of imidacloprid on Drosophila melanogaster Dα1/Dβ1 nicotinic acetylcholine receptors expressed in Xenopus laevis oocytes

Cited by 4 publications

References 31 publications

Mutation V65I in the β1 Subunit of the Nicotinic Acetylcholine Receptor Confers Neonicotinoid and Sulfoxaflor Resistance in Insects

Mutation V65I in the β1 Subunit of the Nicotinic Acetylcholine Receptor Confers Neonicotinoid and Sulfoxaflor Resistance in Insects

Transcriptome Analysis Reveals Differentially Expressed circRNAs Associated with Fecundity in Small-Tail Han Sheep Thyroid with Different FecB Genotypes

Generation of Mutants from the 57B Region of Drosophila melanogaster

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