Effects of colchicine on carrot callus — growth and energy status

Sloan, Margaret E.; Camper, N. D.

doi:10.1007/bf02318905

Cited by 5 publications

(1 citation statement)

References 19 publications

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“…L.), poinsettias (Euphorbia pulcherrima Willd. ex Klotzsch), and Watsonia lepida N. E. Brown (Ascough et al, 2008;Pickens et al, 2006;Sloan and Camper, 1981). Callus production slightly increased (compared with the control media) and remained generally constant in culture media containing 30 or 60 mM oryzalin, regardless of exposure time, but steadily declined over time for medium containing 120 mM oryzalin ( Table 2).…”

Section: Resultsmentioning

confidence: 96%

Induced Polyploidy in Diploid Ornamental Ginger (Hedychium muluense R. M. Smith) Using Colchicine and Oryzalin

Sakhanokho¹,

Rajasekaran²,

Kelley³

et al. 2009

horts

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The ploidy level of H. muluense, a diploid (2n = 2x = 34) and dwarf ornamental ginger species, has been determined and is reported for the first time. Oryzalin and colchicine were successfully used to induce polyploidy in Hedychium muluense in vitro. Embryogenic cell lines were treated with oryzalin (30, 60, or 120 μM) and colchicine (2.5, 5, or 10 mm) for 24, 48, or 72 h. The control contained no antimitotic agent. Flow cytometry, chloroplast count, and stomatal frequency were more effective and reliable than stomatal length as methods for assessing ploidy. Overall, oryzalin was more effective than colchicine in inducing polyploidy. The highest induction frequency (15%) of tetraploidy was achieved when embryogenic callus was exposed to 60 μM oryzalin for 72 h. For colchicine, exposure of embryogenic callus to the 2.5 mm colchicine for 24 h was the most effective in creating tetraploid (13%) plants.

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Section: Resultsmentioning

confidence: 96%

Induced Polyploidy in Diploid Ornamental Ginger (Hedychium muluense R. M. Smith) Using Colchicine and Oryzalin

Sakhanokho¹,

Rajasekaran²,

Kelley³

et al. 2009

horts

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Tubulins from Plants, Fungi, and Protists

Morejohn

Fosket

1986

Cell and Molecular Biology of the Cytoskeleton

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Strategies for “minimal growth maintenance” of cell cultures: A perspective on management for extended duration experimentation in the microgravity environment of a space station

Krikorian

1996

Bot. Rev

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How cells manage without gravity and how they change in the absence of gravity are basic questions that only prolonged life on a Space station will enable us to answer. We know from investigations carried out on various kinds of Space vehicles and stations that profound physiological effects can and often to occur. We need to know more of the basic biochemistry and biophysics both of cells and of whole organisms in conditions of reduced gravity. The unique environment of Space affords plant scientists an unusual opportunity to carry out experiments in microgravity, but some major challenges must be faced before this can be done with confidence. Various laboratory activities that are routine on Earth take on special significance and offer problems that need imaginative resolution before even a relatively simple experiment can be reliably executed on a Space station. For example, scientists might wish to investigate whether adaptive or other changes that have occurred in the environment of Space are retained after return to Earth-normal conditions. Investigators seeking to carry out experiments in the low-gravity environment of Space using cultured cells will need to solve the problem of keeping cultures quiescent for protracted periods before an experiment is initiated, after periodic sampling is carried out, and after the experiment is completed. This review gives an evaluation of a range of strategies that can enable one to manipulate cell physiology and curtail growth dramatically toward this end. These strategies include cryopreservation, chilling, reduced oxygen, gel entrapment strategies, osmotic adjustment, nutrient starvation, pH manipulation, and the use of mitotic inhibitors and growth-retarding chemicals. Cells not only need to be rendered quiescent for protracted periods but they also must be recoverable and further grown if it is so desired. Elaboration of satisfactory procedures for management of cells and tissues at "near zero or minimal growth" will have great value and practical consequences for experimentation on Earth as well as in Space. All of the parameters and conditions and procedural details needed to meet all the specific objectives will be the basis of the design and fabrication of cell culture units for use in the Space environment. It is expected that this will be an evolutionary process.

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Effects of colchicine on carrot callus — growth and energy status

Cited by 5 publications

References 19 publications

Induced Polyploidy in Diploid Ornamental Ginger (Hedychium muluense R. M. Smith) Using Colchicine and Oryzalin

Induced Polyploidy in Diploid Ornamental Ginger (Hedychium muluense R. M. Smith) Using Colchicine and Oryzalin

Tubulins from Plants, Fungi, and Protists

Strategies for “minimal growth maintenance” of cell cultures: A perspective on management for extended duration experimentation in the microgravity environment of a space station

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