Effects of Cold Atmospheric Plasma Pre-Treatment of Titanium on the Biological Activity of Primary Human Gingival Fibroblasts

Gund, Madline P.; Naim, Jusef; Lehmann, Antje; Hannig, Matthias; Linsenmann, Constanze; Schindler, Axel; Rupf, Stefan

doi:10.3390/biomedicines11041185

Cited by 3 publications

(5 citation statements)

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“…The second contribution by the research group, "Cold Atmospheric Plasma Improves the Colonization of Titanium with Primary Human Osteoblasts: An In Vitro Study", by Madline P. Gund et al, investigates the effects of cold atmospheric plasma (CAP) treatment on titanium surfaces and its subsequent impact on primary human osteoblast colonization. The findings contribute to the growing body of evidence supporting the utility of cold atmospheric plasmas in biomedical applications, particularly in enhancing osteoblast colonization on implant surfaces [21].…”

mentioning

confidence: 57%

“…Madline P. Gund et al's "Effects of Cold Atmospheric Plasma Pre-Treatment of Titanium on the Biological Activity of Primary Human Gingival Fibroblasts" explores the benefits of CAP treatment on titanium surfaces. The study suggests that CAP-treated titanium exhibits enhanced fibroblast coverage without altering biological behavior, offering insights into potential applications in implantology [21].…”

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confidence: 89%

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Plasma Applications in Biomedicine: A Groundbreaking Intersection between Physics and Life Sciences

Suschek

2024

Biomedicines

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confidence: 57%

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confidence: 89%

Plasma Applications in Biomedicine: A Groundbreaking Intersection between Physics and Life Sciences

Suschek

2024

Biomedicines

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“…The effects have previously been studied on primary human gingival fibroblasts and have shown that pre-treatment of titanium surfaces leads to improved initial adhesion of these cells [ 6 ]. To the best of our knowledge, this is the first study to demonstrate beneficial effects of cold atmospheric plasma to condition micro-structured titanium on the early attachment of primary human osteoblasts.…”

Section: Discussionmentioning

confidence: 99%

“…The peri-implant tissue acts as a barrier and protects against oral bacteria. Contamination of the implant surface by microorganisms can be prevented, thus avoiding inflammation of the surrounding tissue and bone [ 6 , 7 ].…”

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confidence: 99%

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Cold Atmospheric Plasma Improves the Colonization of Titanium with Primary Human Osteoblasts: An In Vitro Study

Gund,

Naim,

Lehmann

et al. 2024

Biomedicines

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Several studies have shown that cold atmospheric plasma (CAP) treatment can favourably modify titanium surfaces to promote osteoblast colonization. The aim of this study was to investigate the initial attachment of primary human osteoblasts to plasma-treated titanium. Micro-structured titanium discs were treated with cold atmospheric plasma followed by the application of primary human osteoblasts. The microwave plasma source used in this study uses helium as a carrier gas and was developed at the Leibniz Institute for Surface Modification in Leipzig, Germany. Primary human osteoblasts were analyzed by fluorescence and cell biological tests (alkaline phosphatase activity and cell proliferation using WST-1 assay). The tests were performed after 4, 12, and 24 h and showed statistically significant increased levels of cell activity after plasma treatment. The results of this study indicate that plasma treatment improves the initial attachment of primary human osteoblasts to titanium. For the first time, the positive effect of cold atmospheric plasma treatment of micro-structured titanium on the initial colonization with primary human osteoblasts has been demonstrated. Overall, this study demonstrates the excellent biocompatibility of micro-structured titanium. The results of this study support efforts to use cold atmospheric plasmas in implantology, both for preimplantation conditioning and for regeneration of lost attachment due to peri-implantitis.

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Cold atmospheric plasma activation of human gingival fibroblasts for improved wound healing

Silva,

Marques,

Brito da Cruz

et al. 2024

J. Phys. D: Appl. Phys.

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Soft tissue regeneration plays a crucial role after oral surgery, as the successful healing of the soft tissue is a primary indicator of an efficacious intervention. Cold atmospheric plasma has recently emerged as a promising therapeutic modality, exhibiting notable effects on cell migration and proliferation. Despite its potential, the dental application of cold atmospheric plasma remains underexplored. This in vitro study aims to elucidate the impact of cold atmospheric plasma activated media on human gingival fibroblast responses, for future wound healing strategies. The study was divided into four parts: initial characterization of plasma Jet, assessment of cell concentration, exploration of treatment distance effects, and treatment time dynamics. Human gingival fibroblasts were exposed to complete DMEM medium (without sodium pyruvate) activated with cold atmospheric plasma at treatment distances of 2, 5, 7, and 9 mm, and treatment times of 15, 60, 120, 180, and 300 s for 1, 2 and 3 days of culture. The cell viability was evaluated using resazurin-based method, while wound healing dynamics was assessed via the scratch assay technique using phase-contrast microscopy. The cell morphology was characterized through fluorescence microscopy using propidium iodide and phalloidin staining, complemented by scanning electron microscopy. The results revealed that treatment distances and exposure times can influence the cell behaviour depending on the cell concentration. For the selected concentration of 1 x 104 cells/mL, the treatment distance of 9 mm appeared to enhance human gingival fibroblast viability compared to a 2 mm treatment distance and the control group. The images reveal adhered cells with typical fibroblast pattern. However, no differences between the treatment times of 15 s and 180 s were observed. The observed results evidence that the exposure of the medium to the cold atmospheric plasma device promoted an increase of cell viability, proliferation, and attachment in human gingival fibroblasts.

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Effects of Cold Atmospheric Plasma Pre-Treatment of Titanium on the Biological Activity of Primary Human Gingival Fibroblasts

Cited by 3 publications

References 34 publications

Plasma Applications in Biomedicine: A Groundbreaking Intersection between Physics and Life Sciences

Plasma Applications in Biomedicine: A Groundbreaking Intersection between Physics and Life Sciences

Cold Atmospheric Plasma Improves the Colonization of Titanium with Primary Human Osteoblasts: An In Vitro Study

Cold atmospheric plasma activation of human gingival fibroblasts for improved wound healing

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