Effects of cold atmospheric pressure plasma and disinfecting agents on <scp><i>Candida albicans</i></scp> in root canals of extracted human teeth

Kerlikowski, Anne; Matthes, Rutger; Pink, Christiane; Steffen, H.; Schlüter, Rabea; Holtfreter, Birte; Weltmann, Klaus‐Dieter; Woedtke, Thomas von; Kocher, Thomas; Jablonowski, Lukasz

doi:10.1002/jbio.202000221

Cited by 14 publications

(23 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kerlikowski et al used an Ar/O 2 jet device to treat C. albicans in root canals. The reduction achieved was 2.1 CFU in less than 600 s [ 65 ]. He/O 2 resulted in total inactivation of a C. albicans and a C. krusei biofilm within 60 s [ 72 ], whereas another group of authors showed a reduction of 0.4 to 0.8 log 10 CFU dependent on the amount of oxygen admixed [ 70 ].…”

Section: Resultsmentioning

confidence: 99%

“…The antifungal effect of CAP on C. albicans was investigated in 14 studies, 13 on plain surfaces, and 1 in an endodontic model [ 65 ]. In accordance with the findings from the bacterial experiments, the reduction increased with in a time-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

“…When treating a C. albicans biofilm, CAP showed a better antifungal effect compared to CHX 2% and NaOCl in a 6 and 12 min application time [ 65 ]. Against a multi-species, ex-vivo biofilm NaOCl was far more effective than CAP resulting in a reduction of 4.5 log 10 compared to 1.0 log 10 , respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Another interesting aspect of plasma application is the reduction of the surface tension [ 101 ]. An additional advantageous effect on the irrigants’ disinfecting capability [ 65 , 102 ] and an improved adhesion of the restorative material, especially after NaOCl rinsing [ 77 , 103 ], makes CAP a promising extension to the established endodontic treatment protocols. In terms of oral candidiasis, BALB/c mice were infected with C. albicans and treated with CAP 5 days consecutively.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

The Antimicrobial Effect of Cold Atmospheric Plasma against Dental Pathogens—A Systematic Review of In-Vitro Studies

et al. 2021

View full text Add to dashboard Cite

Interest in the application of cold atmospheric plasma (CAP) in the medical field has been increasing. Indications in dentistry are surface modifications and antimicrobial interventions. The antimicrobial effect of CAP is mainly attributed to the generation of reactive oxygen and reactive nitrogen species. The aim of this article is to systematically review the available evidence from in-vitro studies on the antimicrobial effect of CAP on dental pathogens. A database search was performed (PubMed, Embase, Scopus). Data concerning the device parameters, experimental set-ups and microbial cultivation were extracted. The quality of the studies was evaluated using a newly designed assessment tool. 55 studies were included (quality score 31–92%). The reduction factors varied strongly among the publications although clusters could be identified between groups of set pathogen, working gases, and treatment time intervals. A time-dependent increase of the antimicrobial effect was observed throughout the studies. CAP may be a promising alternative for antimicrobial treatment in a clinically feasible application time. The introduced standardized protocol is able to compare the outcome and quality of in-vitro studies. Further studies, including multi-species biofilm models, are needed to specify the application parameters of CAP before CAP should be tested in randomized clinical trials.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The Antimicrobial Effect of Cold Atmospheric Plasma against Dental Pathogens—A Systematic Review of In-Vitro Studies

et al. 2021

View full text Add to dashboard Cite

show abstract

“…This single-electrode tiny plasma jet without the major drawbacks of conventional plasma jet (high voltage, high flow rate, reactive species delivery, plasma jet volume, and discharge formation in the organ) will have more potential biomedical applications, especially for in vivo clinical applications. For example, plasma disinfects bacteria in the oral cavity, such as Enterococcus faecalis and Candida albicans [36,37]. This reliable and user-friendly tiny plasma jet could be directed manually to target a root canal for disinfection.…”

Section: Resultsmentioning

confidence: 99%

Tiny Cold Atmospheric Plasma Jet for Biomedical Applications

2021

View full text Add to dashboard Cite

Conventional plasma jets for biomedical applications tend to have several drawbacks, such as high voltages, high gas delivery, large plasma probe volume, and the formation of discharge within the organ. Therefore, it is challenging to employ these jets inside a living organism’s body. Thus, we developed a single-electrode tiny plasma jet and evaluated its use for clinical biomedical applications. We investigated the effect of voltage input and flow rate on the jet length and studied the physical parameters of the plasma jet, including discharge voltage, average gas and subject temperature, and optical emissions via spectroscopy (OES). The interactions between the tiny plasma jet and five subjects (de-ionized (DI) water, metal, cardboard, pork belly, and pork muscle) were studied at distances of 10 mm and 15 mm from the jet nozzle. The results showed that the tiny plasma jet caused no damage or burning of tissues, and the ROS/RNS (reactive oxygen/nitrogen species) intensity increased when the distance was lowered from 15 mm to 10 mm. These initial observations establish the tiny plasma jet device as a potentially useful tool in clinical biomedical applications.

show abstract

The potential of gas plasma technology for targeting breast cancer

Bekeschus

Saadati

Emmert

2022

Clinical & Translational Med

View full text Add to dashboard Cite

Despite therapeutic improvements in recent years, breast cancer remains an often fatal disease. In addition, breast cancer ulceration may occur during late stages, further complicating therapeutic or palliative interventions. In the past decade, a novel technology received significant attention in the medical field: gas plasma. This topical treatment relies on the partial ionization of gases that simultaneously produce a plethora of reactive oxygen and nitrogen species (ROS/RNS). Such local ROS/RNS overload inactivates tumour cells in a non‐necrotic manner and was recently identified to induce immunogenic cancer cell death (ICD). ICD promotes dendritic cell maturation and amplifies antitumour immunity capable of targeting breast cancer metastases. Gas plasma technology was also shown to provide additive toxicity in combination with radio and chemotherapy and re‐sensitized drug‐resistant breast cancer cells. This work outlines the assets of gas plasma technology as a novel tool for targeting breast cancer by summarizing the action of plasma devices, the roles of ROS, signalling pathways, modes of cell death, combination therapies and immunological consequences of gas plasma exposure in breast cancer cells in vitro, in vivo, and in patient‐derived microtissues ex vivo.

show abstract

Effects of cold atmospheric pressure plasma and disinfecting agents on Candida albicans in root canals of extracted human teeth

Cited by 14 publications

References 39 publications

The Antimicrobial Effect of Cold Atmospheric Plasma against Dental Pathogens—A Systematic Review of In-Vitro Studies

The Antimicrobial Effect of Cold Atmospheric Plasma against Dental Pathogens—A Systematic Review of In-Vitro Studies

Tiny Cold Atmospheric Plasma Jet for Biomedical Applications

The potential of gas plasma technology for targeting breast cancer

Contact Info

Product

Resources

About