Effects of Cold Storage on Serum Antibodies of the Goldfish

Rio, G. J.; Receo, Rose A.

doi:10.1577/1548-8640(1971)33[37:eocsos]2.0.co;2

Cited by 1 publication

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“…For juveniles, blood from several fish (see Table I) was pooled in order to obtain 1 0 0~1 of serum for antibody titrations. Test sera were always used fresh because freezing reduces the antibody levels (Rio & Reco, 1971). Heat inactivation of sera to remove heat-labile complement activity (Cushing, 1945) was carried out in some of the preliminary experiments but was later abandoned because the lytic effects of pre-existing complement proved to be negligible.…”

Section: Serum Collection and Antibody Titrationmentioning

confidence: 99%

The immune system of juvenile thick‐lipped grey mullet, Chelon labrosus Risso: antibody responses to soluble protein antigens

Mughal

Manning

1986

Journal of Fish Biology

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Juvenile thick-lipped grey mullet, Chelon lubrosus, believed to be about 6-7 months old, possessed well developed lymphoid organs including a clearly differentiated thymus with distinct cortical and medullary zones. However, unlike older fish, the juvenile mullet usually failed to produce antibody in response to a single injection of classical thymus-dependent antigen (using the soluble proteins human gamma globulin or keyhole limpet haemocyanin). Prior priming of the juvenile fish with antigen was found to potentiate antibody production following challenge with a second dose of the antigen in adjuvant, priming by oral administration being equally a s effective as priming by injection. Neither juvenile nor adult mullet produced any significant level of antibody against ovalbumin.The results suggest that, despite their apparently well differentiated lymphoid organs, juvenile mullet still show a certain level of immaturity in their antibody responses to soluble proteins; also that immunization can improve their ability to respond.

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Section: Serum Collection and Antibody Titrationmentioning

confidence: 99%