Effects of cold storage on the fitness of Tamarixia radiata, a dominant parasitoid of Asian citrus psyllid Diaphorina citri

Zhang, Lihe; Lu, Zi-Tong; Guo, Chang-Fei; Shen, Zu-Le; Wang, Ziqi; Sang, Wen; Qiu, Bao‐Li

doi:10.1016/j.cropro.2019.104988

Cited by 11 publications

(12 citation statements)

References 39 publications

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“…Most past studies focused on cold storage of parasitized eggs via diapause and quiescence, but researchers seldom examined storage of unparasitized host eggs (Cagnotti et al., 2018; Colinet & Boivin, 2011; Lu et al., 2019; Wu et al., 2018; Zhang et al., 2020). Previous evidence suggests stored host eggs have negative impacts on parasitoid fitness that vary with storage temperature and duration (Peverieri et al., 2015; Wu et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

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Cold storage of Spodoptera litura eggs and Telenomus remus adults for improving mass‐rearing efficiency

Chen

Zhang

Xiao-yu

et al. 2022

J Applied Entomology

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Telenomus remus (Nixon) has shown promising potential as a biological control agent for the major invasive agricultural pest, Spodoptera frugiperda (J. E. Smith). Application of biological control programmes in the field requires an efficient mass‐rearing system to provide sufficient numbers of beneficial insects. The short longevity of parasitoid adults and limited period of availability of host eggs are two factors affecting the mass‐rearing efficiency of T. remus. Using Spodoptera litura (Fabricius) eggs as an alternative host, the effects of cold storage of S. litura eggs and T. remus adults at 5°C, 8°C and 11°C for different time durations on the fitness of the parasitoid were investigated to identify the most suitable storage conditions. Results showed that T. remus can successfully exploit stored S. litura eggs, but the number of parasitized eggs decreased with increased egg storage duration and/or reduced storage temperature. For refrigerated adults, the survival rate declined gradually with longer storage duration, and the females' survival rate was higher than the males' at any treatment. Similarly, parasitism capacity of stored adults declined significantly with prolonged storage. Female adult longevity was significantly shorter than the control for all treatments. However, the offspring emergence rate and percentage of females were barely affected by cold storage. In conclusion, S. litura eggs can be stored at 11°C for up to 9 days and adult T. remus can be stored at 8°C or 11°C for up to 7 days without significant losses. Data obtained from this study will help coordinate different aspects of T. remus production, decreasing costs and improving the biocontrol materials' availability.

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Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Cold storage of Spodoptera litura eggs and Telenomus remus adults for improving mass‐rearing efficiency

Chen

Zhang

Xiao-yu

et al. 2022

J Applied Entomology

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“…The biological characteristics of T. radiata were elucidated in our previous studies, including the effects of temperature on its development and its host preference behavior to different stages of ACP, among others [ 19 , 38 ]. However, the reference genes of T. radiata are yet to be evaluated, making it difficult to rely upon RT-qPCR to study the gene expression patterns in this parasitoid.…”

Section: Discussionmentioning

confidence: 99%

“…Hence, devising an alternative control strategy for ACP is very important and essential. Tamarixia radiata Waterston (Hymenoptera: Eulophidae) is the most effective natural enemy of ACPand it has been successfully used as a biological control agent of ACP in various countries [ 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. Recently, several studies investigated the relationship between venomous proteins of the parasitoid wasp and its host insect prey [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive Assessment of Candidate Reference Genes for Gene Expression Studies Using RT-qPCR in Tamarixia radiata, a Predominant Parasitoid of Diaphorina citri

Guo

Pan

Zhang

et al. 2020

Genes

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Tamarixia radiata (Waterston) is a predominant parasitoid of the Asian citrus psyllid (ACP), a destructive citrus pest and vector of huanglongbing (HLB) disease in the fields of southern China. To explore the functioning of target genes in T. radiata, the screening of specific reference genes is critical for carrying out the reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) under different experimental conditions. However, no reference gene(s) for T. radiata has yet been reported. Here, we selected seven housekeeping genes of T. radiate and evaluated their stability under the six conditions (developmental stage, sex, tissue, population, temperature, diet) by using RefFinder software, which contains four different programs (geNorm, ΔCt, BestKeeper, and NormFinder). Pairwise variation was analyzed by geNorm software to determine the optimal number of reference genes during the RT-qPCR analysis. The results reveal better reference genes for differing research foci: 18S and EF1A for the developmental stage; PRS18 and EF1A for sex, PRS18 and RPL13 for different tissues (head, thorax, abdomen); EF1A and ArgK between two populations; β-tubulin and EF1A for different temperatures (5, 15, 25, 35 °C); and ArgK and PRS18 for different feeding diets. Furthermore, when the two optimal and two most inappropriate reference genes were chosen in different temperatures and tissue treatments, respectively, the corresponding expression patterns of HSP70 (as the reporter gene) differed substantially. Our study provides, for the first time, a more comprehensive list of optimal reference genes from T. radiata for use in RT-qPCR analysis, which should prove beneficial for subsequent functional investigations of target gene(s) in this natural enemy of ACP.

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Optimal cold storage protocol for Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae), a biological control agent for coleopteran pests in stored products

Yang,

Zhang,

et al. 2023

Pest Management Science

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BACKGROUNDAnisopteromalus calandrae (Howard) is a solitary ectoparasitoid with wide‐ranging potential applications as a natural biological control agent against various coleopterous pests in food warehouses. Implementing an effective cold storage program is crucial for extending the shelf life of biological control agents and ensuring their stable and abundant supply. Herein, we attempted to determine the optimal cold storage conditions for A. calandrae by investigating the effect of cold storage at three different temperatures (7, 13, and 19°C) for 7, 21, and 35 days on four developmental stages (late‐instar larvae, early‐stage pupae, mid‐stage pupae, and 2‐day‐old adults). Additionally, we explored the maximum cold storage potential by observing early‐stage pupae stored at 13°C for various durations (30, 60, 90, 120, and 150 days).RESULTSThe most suitable cold storage temperature for the early‐stage pupae of A. calandrae was 13°C, and the highest adult emergence rate (98.3%) was after 90 days of storage at 13°C. Furthermore, we did not find any significant effect on longevity (female: 44.3 days; male: 38.1 days) or fecundity (121.7 wasps). The female ratio ranged from 43.5% to 50.8%. More importantly, cold storage did not adversely affect the developmental duration or fecundity of the offspring.CONCLUSIONThis study offers crucial insights for managing A. calandrae populations under laboratory conditions and lays the foundation for potential industrial production and development.This article is protected by copyright. All rights reserved.

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Effects of cold storage on the fitness of Tamarixia radiata, a dominant parasitoid of Asian citrus psyllid Diaphorina citri

Cited by 11 publications

References 39 publications

Cold storage of Spodoptera litura eggs and Telenomus remus adults for improving mass‐rearing efficiency

Cold storage of Spodoptera litura eggs and Telenomus remus adults for improving mass‐rearing efficiency

Comprehensive Assessment of Candidate Reference Genes for Gene Expression Studies Using RT-qPCR in Tamarixia radiata, a Predominant Parasitoid of Diaphorina citri

Optimal cold storage protocol for Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae), a biological control agent for coleopteran pests in stored products

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