Effects of Collagen Grafting on Cell Behaviors in BCP Scaffold with Interconnected Pore Structure

Abstract

BackgroundThis study was to investigate the effect of collagen grafted porous biphasic calcium phosphate (BCP) on cell attachment, proliferation, and differentiation. Porous BCP scaffolds with interconnected micropore structure were prepared with were prepared and then grafted with a collagen type I. The hydroxyapatite (HA) and β-tricalcium phosphate (TCP) ratio of the TCP scaffolds was about 60/40 and the collagen was crosslinked on the TCP scaffold surface (collagen-TCP).ResultsThe sintered BCP scaffolds showed fully interconnected micropore structures with submicron-sized grains. The collagen crosslinking in the scaffolds was conducted using the the N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (NHS) crosslinking method. The cell proliferation of collagen-BCP scaffolds showed a similar result to that of the BCP scaffolds. However, osteoblastic differentiation and cell attachment increased in the collagen-BCP scaffolds.ConclusionsCollagen-BCP scaffold improved the cell attachment ability in early phase and osteoblastic differentiation.

Bone regeneration using three‐dimensional hexahedron channel structured BCP block in rabbit calvarial defects

“…The scaffold also showed favorable volume maintenance throughout the experimental period without ingrowth of overlying soft connective tissue. Moreover, it exhibited the biocompatible property without the inflammation in vivo, similar to the experimental in vivo findings of the previous studies using BCP …”

“…Moreover, it exhibited the biocompatible property without the inflammation in vivo, similar to the experimental in vivo findings of the previous studies using BCP. 9,19 In micro-CT analysis, it is hard to distinguish the grayscale values of fibrovascular connective tissue, new bone, old bone and graft materials. Micro-CT analysis was performed to grossly determine the tendency of new bone formation and total volume rather than to analyze the precise volume of new bone and graft materials.…”

Hard Tissue Augmentation of Aged Bone by Means of a Tin-Free PLLA-PCL Co-Polymer Exhibiting in vivo Anergy and Long-Term Structural Stability

“…2f). Cells on PbP commenced growth in the form of colonies shortly after seeding, while on the commercially available TCP ceramics Cerasorb® M (Curasan) [19-21], cells proliferated in a dispersed manner. The results of these initial tests prompted us to primarily test scaffold material with high porosity, which in due course of the experimental evaluation was also selected for in vivo experimentation.…”

Synthetic osteoplastic materials for alveolar bone augmentation before dental implantation

“…-по размеру пор частиц материала [26,38]: с макропорами (диаметр>100 мкм), с микропорами (диаметр <100 мкм); -по наличию пор в гранулах материала [5,10]: пористая (резорбируемая), непористая (нерезорбируемая); -по форме выпуска препарата [5,10]: блоки, гранулы, порошки, гели.…”

“…Достаточно часто применяется двухфазный фосфат кальция, состоящий из смеси β-ТКФ и ГА. Биологическая активность и резорбция двухфазной керамики могут регулироваться путем изменения процентного соотношения частиц ГА и β-ТКФ [23,38]. Отмечается также, что применение двухфазной керамики с разными соотношениями ГА/β-ТКФ больше способствует образованию костной ткани, чем применение ее компонентов по отдельности [38].…”