Effects of Collagen IV on Neuroblastoma Cell Matrix-Related Functions

Τzinia, Athina K.; Kitsiou, Paraskevi V.; Talamagas, Argiris A.; Georgopoulos, Angelique; Tsilibary, Effie-Photini C

doi:10.1006/excr.2001.5463

Cited by 15 publications

(8 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Supporting our results, it has been shown that MMP-1,-2 and-9 are highly expressed in human breast carcinoma cell lines (Iwata et al, 1996), and MMP-1,-2,-3,-9 and-11 are expressed in increased levels in breast cancer tumor as opposed to their expression in normal breast or benign breast disease (Benaud et al, 1998;Curran and Murray, 1999;Curran and Murray, 2000;Garbett et al, 2000;Kossakowska et al, 1996). In addition, in epithelial, neuronal and melanoma cells, Col-IV plays an important role in migration, Erk phosphorylation and activation of MMPs (Hodgson et al, 2003;Sanders and Basson, 2000;Tzinia et al, 2002). Furthermore, in breast cancer cells, IL-1β promotes metastasis and invasion by a process that require the SHP-2 phosphotyrosine phosphatase activation and an increase in MMP-9 secretion, while type I collagen induces MMP-2 activation (Borrirukwanit et al, 2007;Wang et al, 2005).…”

Section: Discussionmentioning

confidence: 96%

Src kinase regulates metalloproteinase-9 secretion induced by type IV collagen in MCF-7 human breast cancer cells

et al. 2008

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 96%

Src kinase regulates metalloproteinase-9 secretion induced by type IV collagen in MCF-7 human breast cancer cells

et al. 2008

View full text Add to dashboard Cite

“…Flow cytometry To estimate cell surface distribution of integrins, starved cells were treated with TNF-α in the presence or absence of CHX for 24 h. After treatment, surface distribution of integrins was estimated as previously described (Tzinia et al 2002).…”

Section: Methodsmentioning

confidence: 99%

“…Images of western blots were analyzed using image-processing software (Bioprofil Vilber Loumart). For gelatin zymography, aliquots of conditioned media were subjected to SDS-PAGE under non-reducing conditions on 10 % polyacrylamide gels containing 0.1% gelatin as previously described (Tzinia et al 2002).…”

Section: Methodsmentioning

confidence: 99%

TIMP-1 interaction with αvβ3 integrin confers resistance to human osteosarcoma cell line MG-63 against TNF-α-induced apoptosis

2010

View full text Add to dashboard Cite

Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine affecting diverse cellular responses. TNF-α is cytotoxic in many systems, but it can also act as an anti-apoptotic signal to promote cell survival pathways activated through integrins and extracellular matrix components. This is particularly evident in cancer cells. To unravel the basis of resistance to TNF-α-induced apoptosis, human osteosarcoma MG-63 cell line was used. Our data showed that resistance to apoptosis was accompanied by high levels of TIMP-1 expression in part mediated by NF-κB activation, whereas under apoptotic conditions, in the presence of cycloheximide (CHX), TIMP-1 and αvβ3 integrin protein levels were significantly reduced. Silencing TIMP-1 using siRNA led to increased apoptosis following treatment with TNF-α, whereas exogenously-added recombinant TIMP-1 reduced the extent of apoptosis. Immunoprecipitation and confocal microscopy experiments demonstrated that TIMP-1 interacted with αvβ3 integrins. The biological role of this interaction was revealed by the use of echistatin, an antagonist of αvβ3 integrin. In the presence of echistatin, decreased protection against apoptosis by recombinant TIMP-1 was observed.

show abstract

“…In particular Col-IV, the main component of BM, is the first protein that must be degraded in order to produce cell invasion. In epithelial, neuronal and melanoma cells, it has been described that Col-IV plays an important role in migration, Erk tyrosine phosphorylation and activation of matrix metalloproteinases (MMP) (Hodgson et al, 2003;Sanders and Basson, 2000;Tzinia et al, 2002). In contrast, little is known about the cellular functions and the signal transduction pathways mediated by BM components, such as Col-IV, in breast cancer cells.…”

Section: Discussionmentioning

confidence: 93%