Effects of collagen matrix and bioreactor cultivation on cartilage regeneration of a full-thickness critical-size knee joint cartilage defects with subchondral bone damage in a rabbit model

Wang, Kuo Hwa; Wan, Richard; Chiu, Li Hsuan; Tsai, Yu Hui; Fang, Chia Lang; Bowley, John F.; Chen, Kuan Chou; Shih, Hong-Mo; Lai, Wen

doi:10.1371/journal.pone.0196779

Cited by 17 publications

(12 citation statements)

References 48 publications

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“…The implantation of collagen gel and MSCs into an athlete suffering from knee pain resulted in hyaline-like tissue formation and functional recovery of the articular cartilage ( Kuroda et al, 2007 ). The mixture of rabbit chondrocytes with rabbit and rat collagen scaffolds to form neo-RBT (neo-rabbit cartilage) and neo-RAT (neo-rat cartilage) constructs featured cartilage-like repair tissue covering the 5-mm circular, 4-mm deep defects created in the rabbit condyles ( Wang et al, 2018 ).…”

Section: Natural Polymers As Scaffolds For Cartilage Tissue Engineerimentioning

confidence: 99%

Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering

Zhao

et al. 2021

Front. Bioeng. Biotechnol.

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Cartilage, especially articular cartilage, is a unique connective tissue consisting of chondrocytes and cartilage matrix that covers the surface of joints. It plays a critical role in maintaining joint durability and mobility by providing nearly frictionless articulation for mechanical load transmission between joints. Damage to the articular cartilage frequently results from sport-related injuries, systemic diseases, degeneration, trauma, or tumors. Failure to treat impaired cartilage may lead to osteoarthritis, affecting more than 25% of the adult population globally. Articular cartilage has a very low intrinsic self-repair capacity due to the limited proliferative ability of adult chondrocytes, lack of vascularization and innervation, slow matrix turnover, and low supply of progenitor cells. Furthermore, articular chondrocytes are encapsulated in low-nutrient, low-oxygen environment. While cartilage restoration techniques such as osteochondral transplantation, autologous chondrocyte implantation (ACI), and microfracture have been used to repair certain cartilage defects, the clinical outcomes are often mixed and undesirable. Cartilage tissue engineering (CTE) may hold promise to facilitate cartilage repair. Ideally, the prerequisites for successful CTE should include the use of effective chondrogenic factors, an ample supply of chondrogenic progenitors, and the employment of cell-friendly, biocompatible scaffold materials. Significant progress has been made on the above three fronts in past decade, which has been further facilitated by the advent of 3D bio-printing. In this review, we briefly discuss potential sources of chondrogenic progenitors. We then primarily focus on currently available chondrocyte-friendly scaffold materials, along with 3D bioprinting techniques, for their potential roles in effective CTE. It is hoped that this review will serve as a primer to bring cartilage biologists, synthetic chemists, biomechanical engineers, and 3D-bioprinting technologists together to expedite CTE process for eventual clinical applications.

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Section: Natural Polymers As Scaffolds For Cartilage Tissue Engineerimentioning

confidence: 99%

Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering

Zhao

et al. 2021

Front. Bioeng. Biotechnol.

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“…Different types of collagen-based scaffolds have been used in tissue engineering applications [ 91 ]. Collagen scaffolds combined with hydroxyapatite have been used in orthopedic applications, inducing bone and cartilage regeneration [ 95 , 96 ]. Moreover, collagen has been proposed as a drug delivery system (DDS) to release pro-angiogenic factors for wound healing applications and as a natural coating of vascular grafts [ 97 , 98 ].…”

Section: Tevgs Derived From Synthetic Polymersmentioning

confidence: 99%

Future Perspectives in Small-Diameter Vascular Graft Engineering

et al. 2020

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The increased demands of small-diameter vascular grafts (SDVGs) globally has forced the scientific society to explore alternative strategies utilizing the tissue engineering approaches. Cardiovascular disease (CVD) comprises one of the most lethal groups of non-communicable disorders worldwide. It has been estimated that in Europe, the healthcare cost for the administration of CVD is more than 169 billion €. Common manifestations involve the narrowing or occlusion of blood vessels. The replacement of damaged vessels with autologous grafts represents one of the applied therapeutic approaches in CVD. However, significant drawbacks are accompanying the above procedure; therefore, the exploration of alternative vessel sources must be performed. Engineered SDVGs can be produced through the utilization of non-degradable/degradable and naturally derived materials. Decellularized vessels represent also an alternative valuable source for the development of SDVGs. In this review, a great number of SDVG engineering approaches will be highlighted. Importantly, the state-of-the-art methodologies, which are currently employed, will be comprehensively presented. A discussion summarizing the key marks and the future perspectives of SDVG engineering will be included in this review. Taking into consideration the increased number of patients with CVD, SDVG engineering may assist significantly in cardiovascular reconstructive surgery and, therefore, the overall improvement of patients’ life.

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“…It is likely that an ideal construct is to be considered the one made from self-tissue (an autograft), however, the approach has a certain restriction: a replacement of a deep osteochondral defect results in defects forming elsewhere. A promising basis for cartilaginous tissue scaffolds is decellularized matrix of animal cartilaginous tissue proper -xenograft [3][4][5][6][7][8][9][10][11][12][13], which complies with requirements 1 and 3. Decellularization is a multi-stage process of combined chemomechanical treatment [4], after which cartilaginous tissue preparation fails to meet requirement 4.…”

Section: Introductionmentioning

confidence: 99%

“…It should be noted that decellularization without mechanical grinding is rather labor-consuming process; moreover, the remaining collagen matrix appears to be too dense to ensure compliance with requirement 2 [7]. To create material with directed collagen fibers and pores from cartilage powder needs, as a rule, special press molds with programmable temperature change [8,10,11]. Another challenging technique to create channels in collagen matrix was suggested in the study [9], where the needles, 400 µm in diameter, were used.…”

Section: Introductionmentioning

confidence: 99%

Development of a Two-Layer Porous Scaffold Based on Porcine Nasal Septal Cartilage for Orthopedics

Ignatieva¹,

Захаркина²,

Сергеева³

et al. 2021

Sovrem Tehnol Med

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The aim of the study was to design a construct based on a nasal septal cartilage plate providing required cell differentiation in different layers to replace a deep osteochondral defect and develop an algorithm of chemical and physical effect sequence to create nonimmunogenic two-layer porous structure with requisite elasto-mechanical properties.Materials and Methods. The plates derived from porcine nasal septal hyaline cartilage covered by perichondrium were multi-stage treated including freezing, equilibrating in a hypotonic saline solution (type I specimens); trypsinization, point IR-laser effect, re-trypsinization (type II specimens); a stabilizing effect of crosslinking agents -glyceraldehyde/ribose in an acidic medium -washing (type III specimens).For all type specimens: 1) there were established stability parameters (collagen denaturation temperature using a thermal analysis; and Young's modulus using a mechanical analysis);2) there were determined morphological characteristics using light and polarization microscopy with classical staining and nonlinear optical microscopy in second-harmonic generation mode.Results. Thermal, mechanical, and morphological properties in type I specimens slightly differed from those of the initial nasoseptal system. A considerable part of cells had destroyed membranes.In type II specimens, thermal stability of collagen frame was significantly lower; Young's modulus decreased more than fourfold compared to type I specimens. Collagen structure of hyaline cartilage appeared to be disarranged, although the morphological differences of the hyaline part and perichondrium preserved. The construct matrix was almost completely decellularized. Successive exposure to laser radiation and trypsin resulted in the formation of partial holes in the matrix, ~100 µm in diameter.In type III specimens, both the thermal stability of the collagen frame and Young's modulus (E) increased. Glyceraldehyde was more effective than ribose, E having reached the value typical for intact hyaline cartilage. Collagen fibers in type III specimens were thicker than in type I and II specimens. The morphological differences of the hyaline part and perichondrium and partial holes were preserved. Conclusion.Due to sequential treatment by salts, trypsin, IR-laser radiation, and nontoxic crosslinking agents, nasal septal cartilage plate forms porous acellular construction consisting of two layers formed by type I (from perichondrium) and type II (from hyaline part) collagen fibers. In the present construction, stability, mechanical properties, and size of the partial holes can be assigned for cell colonization. It enables to use the construction to replace articular cartilage defects.

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Effects of collagen matrix and bioreactor cultivation on cartilage regeneration of a full-thickness critical-size knee joint cartilage defects with subchondral bone damage in a rabbit model

Cited by 17 publications

References 48 publications

Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering

Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering

Future Perspectives in Small-Diameter Vascular Graft Engineering

Development of a Two-Layer Porous Scaffold Based on Porcine Nasal Septal Cartilage for Orthopedics

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