Effects of Colostrum Basic Protein from Colostrum Whey Protein: Increases in Osteoblast Proliferation and Bone Metabolism

Abstract

Colostrum basic protein (CBP) (MW 1-30 kDa) were isolated from bovine colostrum using a series of ultrafiltration processes and their effects on osteoblast cell proliferation and bone metabolism were investigated in cell line and animal models. Treatment with CBP (1,10,100 µg/ml) does-dependently increased cell proliferation of osteoblastic MC3T3 cells. Alkaline phosphastase activity, a marker of osteoblased phenotype, in the cells was also increase after treatment with CBP in a does-dependent manner. Significant increases in bone density were observed in femur of ovariectomized rats which were fed a diet with 1% and 10% CBP, compared to rats fed a normal diet. These results suggest that CBP may increase bone mass and density and be useful for the prevention of bone-related diseases.

Characterization of bacterial populations from Murciano-Granadina goat colostrum

“…Physico-chemical characterisation of colostrum from human (Asakuma et al 2008;Jiménez et al 2008;Lee et al 2007;Maldonado-Barragán et al 2009), cow (Christiansen et al 2010;Tsioulpas et al 2007) and goat breeds (Argüello et al 2006;Górová et al 2011;Quiles et al 1991;Yang et al 2009) has been reported. The presence of high concentrations of nutrients such as proteins, vitamins, minerals, enzymes, antimicrobial peptides and immunoglobulins has been shown.…”

Effect of postpartum time and season on the physicochemical characteristics of Murciano‐Granadina goat colostrum

“…Moreover, bovine colostrum has a high concentration of immune factors (Lee et al . ), which has led some authors to suggest the use of preparations from this product as a supplement in human healthcare (Alexieva et al . ).…”

Modulation of immune cell proliferation and chemotaxis towards CC chemokine ligand (CCL)-21 and CXC chemokine ligand (CXCL)-12 in undenatured whey protein-treated mice

“…The in vitro proliferation of murine splenic lymphocytes was stimulated by WP, but the effect was reduced following hydrolysis using a trypsin/chymotrypsin mixture [46]. In addition, WP increases osteoblast proliferation, as described by Lee et al [47]. Lymphocyte recirculation, which is critical for effective immunity, is tightly regulated by the expression of adhesion molecules and chemoattractant receptors on lymphocytes combined with the spatial and temporal expression of ligands for these receptors by a variety of tissue cells [48].…”